HAUS8_RAT
ID HAUS8_RAT Reviewed; 385 AA.
AC Q5BK57;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=HAUS augmin-like complex subunit 8;
DE AltName: Full=HEC1/NDC80-interacting centrosome-associated protein 1;
DE AltName: Full=Sarcoma antigen NY-SAR-48 homolog;
GN Name=Haus8; Synonyms=Hice1, Ny-sar-48;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Spleen;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Contributes to mitotic spindle assembly, maintenance of
CC centrosome integrity and completion of cytokinesis as part of the HAUS
CC augmin-like complex. {ECO:0000250}.
CC -!- SUBUNIT: Component of the HAUS augmin-like complex. The complex
CC interacts with the gamma-tubulin ring complex and this interaction is
CC required for spindle assembly. Associates with microtubules. The
CC interaction with microtubules is strong during mitosis, while it is
CC weak or absent during interphase. It is unclear whether this
CC interaction is direct or indirect (By similarity). Interacts with EML3
CC (phosphorylated form) and TUBG1 (By similarity).
CC {ECO:0000250|UniProtKB:Q9BT25}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9BT25}.
CC Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
CC {ECO:0000250|UniProtKB:Q9BT25}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000250|UniProtKB:Q9BT25}. Cytoplasm, cytoskeleton, spindle pole
CC {ECO:0000250|UniProtKB:Q9BT25}. Note=During interphase, primarily
CC cytoplasmic and associates with centrosomes and with the mitotic
CC spindles, preferentially at the spindle pole vicinity. During anaphase
CC and telophase, additionally associates with the spindle midzone and
CC midbody, respectively. Localizes to mitotic spindle microtubules (By
CC similarity). {ECO:0000250|UniProtKB:Q9BT25}.
CC -!- SIMILARITY: Belongs to the HAUS8 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH91198.1; Type=Frameshift; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BC091198; AAH91198.1; ALT_FRAME; mRNA.
DR RefSeq; NP_001020142.2; NM_001024971.2.
DR AlphaFoldDB; Q5BK57; -.
DR SMR; Q5BK57; -.
DR IntAct; Q5BK57; 6.
DR STRING; 10116.ENSRNOP00000038415; -.
DR PaxDb; Q5BK57; -.
DR PRIDE; Q5BK57; -.
DR GeneID; 290626; -.
DR KEGG; rno:290626; -.
DR UCSC; RGD:1311565; rat.
DR CTD; 93323; -.
DR RGD; 1311565; Haus8.
DR VEuPathDB; HostDB:ENSRNOG00000052038; -.
DR eggNOG; ENOG502S04A; Eukaryota.
DR HOGENOM; CLU_060977_0_0_1; -.
DR InParanoid; Q5BK57; -.
DR OMA; TAKMEHN; -.
DR OrthoDB; 809178at2759; -.
DR PhylomeDB; Q5BK57; -.
DR TreeFam; TF332998; -.
DR Reactome; R-RNO-2565942; Regulation of PLK1 Activity at G2/M Transition.
DR Reactome; R-RNO-380259; Loss of Nlp from mitotic centrosomes.
DR Reactome; R-RNO-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-RNO-380284; Loss of proteins required for interphase microtubule organization from the centrosome.
DR Reactome; R-RNO-380320; Recruitment of NuMA to mitotic centrosomes.
DR Reactome; R-RNO-5620912; Anchoring of the basal body to the plasma membrane.
DR Reactome; R-RNO-8854518; AURKA Activation by TPX2.
DR PRO; PR:Q5BK57; -.
DR Proteomes; UP000002494; Chromosome 16.
DR Bgee; ENSRNOG00000052038; Expressed in thymus and 19 other tissues.
DR ExpressionAtlas; Q5BK57; baseline and differential.
DR Genevisible; Q5BK57; RN.
DR GO; GO:0005813; C:centrosome; ISO:RGD.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0070652; C:HAUS complex; ISS:UniProtKB.
DR GO; GO:1990498; C:mitotic spindle microtubule; ISS:UniProtKB.
DR GO; GO:0005880; C:nuclear microtubule; IBA:GO_Central.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007098; P:centrosome cycle; ISS:UniProtKB.
DR GO; GO:0051225; P:spindle assembly; ISS:UniProtKB.
PE 2: Evidence at transcript level;
KW Acetylation; Cell cycle; Cell division; Coiled coil; Cytoplasm;
KW Cytoskeleton; Microtubule; Mitosis; Phosphoprotein; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q9BT25"
FT CHAIN 2..385
FT /note="HAUS augmin-like complex subunit 8"
FT /id="PRO_0000319939"
FT REGION 1..78
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 107..128
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 356..385
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 131..248
FT /evidence="ECO:0000255"
FT COMPBIAS 37..70
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q9BT25"
FT MOD_RES 100
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9BT25"
SQ SEQUENCE 385 AA; 42332 MW; AB10EBAA5E962407 CRC64;
MAGASERDAG KPAASGAGAV PKTKGRKVQG GRVVESRYLQ YDKKTKKVSV AAKGEKPPTE
GRKASTVPRS REESKVMGTS NLQSTMLEGH GLNPPDLDLS AIDDKSMSRK APQLERSVAG
TDKSTSLLRP DQKRTLRKKR RDLQETMDMM ESQTLLMTLL SVKVENNLAL LEEKAEKDLA
AMCHEKERLQ RQALELRRQL LLRQKHQELA AALDAQTEVL SPLPPVLERF KEEYKTLGRA
LDTTRHELSM QAVHMEGSGQ ELLDDLEPAL RTTLQLLGDL SICSPEDSAQ VQGASTQQPG
ASAQLNCLLK ELKGLVAEKD LELCRLVSQV VELSSQASKE AALTNQEVWE EAQGTLTSSQ
GYFSPDVRKD HSPTQDRTNS SSLDP
