HAUS4_MOUSE
ID HAUS4_MOUSE Reviewed; 363 AA.
AC Q8BFT2; Q99KI1;
DT 25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=HAUS augmin-like complex subunit 4;
GN Name=Haus4; Synonyms=D14Ertd500e;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Contributes to mitotic spindle assembly, maintenance of
CC centrosome integrity and completion of cytokinesis as part of the HAUS
CC augmin-like complex. {ECO:0000250}.
CC -!- SUBUNIT: Component of the HAUS augmin-like complex. The complex
CC interacts with the gamma-tubulin ring complex and this interaction is
CC required for spindle assembly (By similarity). Interacts with EML3
CC (phosphorylated at 'Thr-882') (By similarity).
CC {ECO:0000250|UniProtKB:Q9H6D7}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000250|UniProtKB:Q9H6D7}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000250|UniProtKB:Q9H6D7}. Note=Localizes to
CC interphase centrosomes and to mitotic spindle microtubules.
CC {ECO:0000250|UniProtKB:Q9H6D7}.
CC -!- SIMILARITY: Belongs to the HAUS4 family. {ECO:0000305}.
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DR EMBL; AK079161; BAC37564.1; -; mRNA.
DR EMBL; AK079852; BAC37767.1; -; mRNA.
DR EMBL; BC004644; AAH04644.1; -; mRNA.
DR EMBL; BC011095; AAH11095.1; -; mRNA.
DR CCDS; CCDS27092.1; -.
DR RefSeq; NP_663437.2; NM_145462.2.
DR RefSeq; XP_006518908.1; XM_006518845.3.
DR AlphaFoldDB; Q8BFT2; -.
DR SMR; Q8BFT2; -.
DR BioGRID; 230105; 33.
DR CORUM; Q8BFT2; -.
DR IntAct; Q8BFT2; 29.
DR STRING; 10090.ENSMUSP00000022784; -.
DR PhosphoSitePlus; Q8BFT2; -.
DR EPD; Q8BFT2; -.
DR MaxQB; Q8BFT2; -.
DR PaxDb; Q8BFT2; -.
DR PeptideAtlas; Q8BFT2; -.
DR PRIDE; Q8BFT2; -.
DR ProteomicsDB; 270889; -.
DR Ensembl; ENSMUST00000022784; ENSMUSP00000022784; ENSMUSG00000022177.
DR GeneID; 219072; -.
DR KEGG; mmu:219072; -.
DR UCSC; uc007twh.2; mouse.
DR CTD; 54930; -.
DR MGI; MGI:1261794; Haus4.
DR VEuPathDB; HostDB:ENSMUSG00000022177; -.
DR eggNOG; ENOG502QW4I; Eukaryota.
DR GeneTree; ENSGT00390000014634; -.
DR HOGENOM; CLU_065166_0_0_1; -.
DR InParanoid; Q8BFT2; -.
DR OMA; NWALKEF; -.
DR OrthoDB; 1168055at2759; -.
DR PhylomeDB; Q8BFT2; -.
DR TreeFam; TF330353; -.
DR Reactome; R-MMU-2565942; Regulation of PLK1 Activity at G2/M Transition.
DR Reactome; R-MMU-380259; Loss of Nlp from mitotic centrosomes.
DR Reactome; R-MMU-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-MMU-380284; Loss of proteins required for interphase microtubule organization from the centrosome.
DR Reactome; R-MMU-380320; Recruitment of NuMA to mitotic centrosomes.
DR Reactome; R-MMU-5620912; Anchoring of the basal body to the plasma membrane.
DR Reactome; R-MMU-8854518; AURKA Activation by TPX2.
DR BioGRID-ORCS; 219072; 23 hits in 74 CRISPR screens.
DR ChiTaRS; Haus4; mouse.
DR PRO; PR:Q8BFT2; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q8BFT2; protein.
DR Bgee; ENSMUSG00000022177; Expressed in seminiferous tubule of testis and 283 other tissues.
DR ExpressionAtlas; Q8BFT2; baseline and differential.
DR Genevisible; Q8BFT2; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0070652; C:HAUS complex; ISS:UniProtKB.
DR GO; GO:1990498; C:mitotic spindle microtubule; ISS:UniProtKB.
DR GO; GO:0051011; F:microtubule minus-end binding; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007098; P:centrosome cycle; ISS:UniProtKB.
DR GO; GO:0051225; P:spindle assembly; ISS:UniProtKB.
DR InterPro; IPR029327; HAUS4.
DR InterPro; IPR026214; HAUS4_met.
DR PANTHER; PTHR16219; PTHR16219; 1.
DR Pfam; PF14735; HAUS4; 1.
DR PRINTS; PR02090; HAUSAUGMINL4.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Cytoplasm; Cytoskeleton; Microtubule; Mitosis;
KW Reference proteome.
FT CHAIN 1..363
FT /note="HAUS augmin-like complex subunit 4"
FT /id="PRO_0000089902"
FT CONFLICT 21
FT /note="G -> V (in Ref. 2; AAH04644/AAH11095)"
FT /evidence="ECO:0000305"
FT CONFLICT 212
FT /note="Q -> H (in Ref. 2; AAH04644/AAH11095)"
FT /evidence="ECO:0000305"
FT CONFLICT 292
FT /note="S -> N (in Ref. 2; AAH04644/AAH11095)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 363 AA; 42197 MW; F10ABB1163E16EE4 CRC64;
MASGDFCAPG EGVEMLLQVC GKQFPPCALT EEDLIQNPRF SKLLLSLSQH VDESGLSLTL
AKEQAQAWSE VRHRKTAWLR YEILQRVIQE LLVDYYVKAQ DTNLTSEDKK FHETLEQRLL
VTELTQLSGP GQETELPPLL GLERTDLLEL MPPSQDFVWM KARLQLEVEE QLKRKCFTLL
CYHDPSSDTD GDTLKAAKVW TLTEVLVREK QQCLEAKSQQ KEQLVLLEKK RTTYSQVLLR
CLALLQRLLQ EHRLQTKSEL DRINAQYLEL KCSAMILRLR MEELKVLSDT YSAEKVEMHR
LIRDRLEGAI RLQEQDLEKS RLVLHTYEAL GEDFESLVRE YTQLRLAADN KRWALQELSK
AQR
