ID   HASR_XENLA              Reviewed;         583 AA.
AC   O57428; Q6AZI0;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   23-MAR-2010, sequence version 2.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Hyaluronan synthase-related protein;
DE   AltName: Full=Hyaluronan synthase-related sequence;
DE            Short=xHAS-rs;
GN   Name=has-rs; Synonyms=hasrs;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], LACK OF CATALYTIC ACTIVITY, AND DEVELOPMENTAL
RP   STAGE.
RC   TISSUE=Gastrula;
RX   PubMed=9442026; DOI=10.1074/jbc.273.4.1923;
RA   Spicer A.P., McDonald J.A.;
RT   "Characterization and molecular evolution of a vertebrate hyaluronan
RT   synthase gene family.";
RL   J. Biol. Chem. 273:1923-1932(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Gastrula;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during a short window during
CC       embryogenesis between at least stages 12 and 19.
CC       {ECO:0000269|PubMed:9442026}.
CC   -!- SIMILARITY: Belongs to the NodC/HAS family. {ECO:0000305}.
CC   -!- CAUTION: Probably inactive because it lacks critical residues conserved
CC       in other family members, including an Asp in the motif DSDT, which is
CC       NSDI in has-rs, and an Arg residue in the motif QXXRW, which is QQTPW
CC       in has-rs. Also inactive in the functional assays of PubMed:9442026.
CC       {ECO:0000305}.
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DR   EMBL; AF015780; AAC60343.1; -; mRNA.
DR   EMBL; BC077983; AAH77983.1; -; mRNA.
DR   RefSeq; NP_001081828.1; NM_001088359.1.
DR   AlphaFoldDB; O57428; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   DNASU; 398074; -.
DR   GeneID; 398074; -.
DR   KEGG; xla:398074; -.
DR   CTD; 398074; -.
DR   Xenbase; XB-GENE-6252031; has-rs.S.
DR   OrthoDB; 332363at2759; -.
DR   Proteomes; UP000186698; Chromosome 3S.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   1: Evidence at protein level;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..583
FT                   /note="Hyaluronan synthase-related protein"
FT                   /id="PRO_0000197182"
FT   TOPO_DOM        1..29
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        30..50
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        51..52
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        53..73
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        74..393
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        394..414
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        415..425
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        426..446
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        447..457
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        458..478
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        479..497
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        498..518
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        519..535
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        536..556
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        557..583
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        270
FT                   /note="H -> R (in Ref. 1; AAC60343)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        440
FT                   /note="L -> V (in Ref. 1; AAC60343)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        518
FT                   /note="I -> V (in Ref. 1; AAC60343)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   583 AA;  67644 MW;  B02B6D2021BCC562 CRC64;
     MENTTDPENI PVSKPKYPTI RRILSQTFRI LLLFSITTAY VLGYQALCHQ GLLITFGLYG
     AAMLLHLLMQ GIFANLEIRR IEKRDGVCSF KKTVALTITG YQENPDYLRQ CLESCKGMKY
     PKDKLKIILV IDGNNEEDVY MMEIFKEVFH GEDVGTYVWQ ENYHTWNIPS EESEDSSSEI
     SSFPWKNEGI QMVEELVRTK RCVCIMQQWG GKREVMYTAF RALGTSVDFI LVCNSDIKLD
     KMATVELVKV LEDDDKNGAV GGDVRVWNRH DSFISFMSSL RYWMVFNMEI ACQSYFDSVT
     YIRGSLGMYR NDILQAFLEF WYNKTFLGTR CPIGDDRFLT NRVLSMGYRT KYSHKSCAYA
     PCQYLRWLNQ QTPWARSYFR MWFCNAQWWH QHHIWMTYES ATGIFFPFFV TAVLIRLMYS
     SSLCNIVWLF LCIQIMSLLL SLYASWQSKK LSMVLMSLYS TLYIIWLLPC QLVALLTIAK
     SDWGTSGRKK VVNNYVPLFS LSIWAAVLLG GLCYSMYIGC RKDWSKPQAN RELYHLLYGC
     AGYMAYWVLM TVIYCVSGSC CKMRSQAVPQ THDITSLSVS LLV