HAP2A_ORYSJ
ID HAP2A_ORYSJ Reviewed; 722 AA.
AC Q5W6B9; A0A0N7KKF6; B9FNK2;
DT 18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Protein HAPLESS 2-A;
DE Flags: Precursor;
GN Name=HAP2A; OrderedLocusNames=Os05g0269500, LOC_Os05g18730;
GN ORFNames=OsJ_17850, OSJNBa0037H03.12;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT "A fine physical map of the rice chromosome 5.";
RL Mol. Genet. Genomics 274:337-345(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP DOMAIN, AND FUNCTION.
RX PubMed=20333238; DOI=10.1371/journal.pgen.1000882;
RA Wong J.L., Leydon A.R., Johnson M.A.;
RT "HAP2(GCS1)-dependent gamete fusion requires a positively charged carboxy-
RT terminal domain.";
RL PLoS Genet. 6:E1000882-E1000882(2010).
RN [8]
RP REVIEW.
RX PubMed=20080406; DOI=10.1016/j.tcb.2009.12.007;
RA Wong J.L., Johnson M.A.;
RT "Is HAP2-GCS1 an ancestral gamete fusogen?";
RL Trends Cell Biol. 20:134-141(2010).
CC -!- FUNCTION: Required for male fertility. Plays a role in pollen tube
CC guidance and successful gamete attachment. Essential for the fusion of
CC gametes during double fertilization, where one male gamete fuses with
CC the egg to produce a zygote, and another male gamete fuses with the
CC central cell to produce the endosperm (PubMed:20333238). Mediates the
CC fusion of cell membranes. Not required for pollen tube outgrowth (By
CC similarity). {ECO:0000250|UniProtKB:F4JP36,
CC ECO:0000269|PubMed:20333238}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000250|UniProtKB:F4JP36}; Single-pass membrane protein
CC {ECO:0000255}. Cell membrane {ECO:0000250|UniProtKB:F4JP36}; Single-
CC pass type I membrane protein {ECO:0000250|UniProtKB:F4JP36}.
CC -!- DOMAIN: The C-terminal domain (583-705) can replace the homologous
CC domain in the Arabidopsis protein, while the N-terminal domain cannot.
CC {ECO:0000269|PubMed:20333238}.
CC -!- MISCELLANEOUS: HAP2/GCS1 family members mediate membrane fusion between
CC gametes in a broad range of eukaryotes, ranging from algae and higher
CC plants to protozoans and cnidaria, suggesting they are derived from an
CC ancestral gamete fusogen. They function similar to viral fusogens, by
CC inserting part of their extracellular domain into the lipid bilayer of
CC an adjoining cell. {ECO:0000303|PubMed:20080406}.
CC -!- SIMILARITY: Belongs to the HAP2/GCS1 family. {ECO:0000305}.
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DR EMBL; AC136522; AAV43963.1; -; Genomic_DNA.
DR EMBL; AP008211; BAF16968.1; -; Genomic_DNA.
DR EMBL; AP014961; BAS93081.1; -; Genomic_DNA.
DR EMBL; CM000142; EEE63042.1; -; Genomic_DNA.
DR EMBL; AK072871; BAG93181.1; -; mRNA.
DR RefSeq; XP_015638244.1; XM_015782758.1.
DR AlphaFoldDB; Q5W6B9; -.
DR SMR; Q5W6B9; -.
DR STRING; 4530.OS05T0269500-01; -.
DR PaxDb; Q5W6B9; -.
DR PRIDE; Q5W6B9; -.
DR EnsemblPlants; Os05t0269500-01; Os05t0269500-01; Os05g0269500.
DR GeneID; 4338255; -.
DR Gramene; Os05t0269500-01; Os05t0269500-01; Os05g0269500.
DR KEGG; osa:4338255; -.
DR eggNOG; ENOG502QREH; Eukaryota.
DR HOGENOM; CLU_022353_1_0_1; -.
DR InParanoid; Q5W6B9; -.
DR OMA; LERDHND; -.
DR OrthoDB; 1196793at2759; -.
DR Proteomes; UP000000763; Chromosome 5.
DR Proteomes; UP000007752; Chromosome 5.
DR Proteomes; UP000059680; Chromosome 5.
DR Genevisible; Q5W6B9; OS.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR InterPro; IPR040326; HAP2/GCS1.
DR InterPro; IPR018928; HAP2/GCS1_dom.
DR PANTHER; PTHR31764; PTHR31764; 1.
DR Pfam; PF10699; HAP2-GCS1; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; Endoplasmic reticulum; Fertilization;
KW Lipid-binding; Membrane; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..722
FT /note="Protein HAPLESS 2-A"
FT /id="PRO_0000416781"
FT TOPO_DOM 25..552
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 553..573
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 574..722
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 598..665
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 680..722
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 600..639
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 640..665
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 36..48
FT /evidence="ECO:0000250|UniProtKB:A4GRC6"
FT DISULFID 129..159
FT /evidence="ECO:0000250|UniProtKB:A4GRC6"
FT DISULFID 141..188
FT /evidence="ECO:0000250|UniProtKB:A4GRC6"
FT DISULFID 160..315
FT /evidence="ECO:0000250|UniProtKB:A4GRC6"
FT DISULFID 162..171
FT /evidence="ECO:0000250|UniProtKB:A4GRC6"
FT DISULFID 298..322
FT /evidence="ECO:0000250|UniProtKB:A4GRC6"
FT DISULFID 435..473
FT /evidence="ECO:0000250|UniProtKB:A4GRC6"
FT CONFLICT 646
FT /note="V -> L (in Ref. 5; EEE63042)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 722 AA; 81604 MW; AB91AA0584CF00D8 CRC64;
MPRRRGTPLP TILLLLAFVG GACGTEILSK SRLESCSHDS DAGGRLKCDR KLVVDLAVPS
GASGGEASLV ARVAGVEEEN DTPSATKSIR DPPVITVSKS ATYALYALTY LDRDVAYRPD
EKYVKTHKCE PYAGAKVVGE CERLWDEKGN VIKQTEPICC PCGPHRVQSK CGDIWSKLTK
GKANTAHCVR FPGDWFHVFG IGAWSLRFSI RVQVKKGSSV WDVVVGPENK TVVSGDNFLR
VKVVGDYTGY TSIPSFEDNY LVTPRKGTGS SQPQDLGNEH SKWMILDRVR FTLDGLECDK
IGVGYEAYRN QPNFCSAPYG SCLGNQLWNF WEYDKRRIDN SQLPLYIVEG RFQRINQHPN
AGAHTFSVGV TEDLNTNLLI ELMADDIEYV YQRSPAKIID IRVPTFEALS QVGIANVTTK
NIGKLESSYS LTFKCSSGIS PVEEQLYTMK PDEVIARSFE LRSTTDQAAM HQCEAILKAS
DFSELDREGY RFSTAATVYN NGAQIGPTND HKKGGFWDSI KALWRNLIDF LTGRLCWTKC
PRLFDFGCHI QYVCIGWILL LLLIPAAVVF LWLLHQEGLF DPLYDWWGLE PDDDYRARRR
HQKGRHHRHH HDHRHRHGHS HGDHHHHYHG GHHQRRRHHH PPAWDVEGHH HDRQQHSHEA
GRNHHRGYGE VVAAGAAPLR LDRASRPGQT EVDAVVEYRE RRSRHERHGG HGHRDGHYSP
SV
