ID   HAND2_XENLA             Reviewed;         210 AA.
AC   P57101; Q05AX2;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Heart- and neural crest derivatives-expressed protein 2;
DE   AltName: Full=Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2;
DE            Short=dHAND;
GN   Name=hand2; Synonyms=dhand;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Embryo;
RX   PubMed=10906469; DOI=10.1016/s0925-4773(00)00334-8;
RA   Angelo S., Lohr J., Lee K.H., Ticho B.S., Breitbart R.E., Hill S.,
RA   Yost H.J., Srivastava D.;
RT   "Conservation of sequence and expression of Xenopus and zebrafish dHAND
RT   during cardiac, branchial arch and lateral mesoderm development.";
RL   Mech. Dev. 95:231-237(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=11084656;
RX   DOI=10.1002/1097-0177(200012)219:4<575::aid-dvdy1075>3.0.co;2-y;
RA   Smith S.J., Kotecha S., Towers N., Mohun T.J.;
RT   "Xenopus Hand2 expression marks anterior vascular progenitors but not the
RT   developing heart.";
RL   Dev. Dyn. 219:575-581(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Fat body;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Essential for cardiac morphogenesis and for the development
CC       of branchial arches. Binds DNA on E-box consensus sequence 5'-CANNTG-3'
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC       protein.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC   -!- TISSUE SPECIFICITY: Heart, liver and spleen.
CC   -!- DEVELOPMENTAL STAGE: At stage 15, faint expression in the antero-
CC       ventral mesoderm. By stage 20, after neural tube closure, expressed in
CC       the ventro-lateral mesoderm of the mid-embryo with punctate expression
CC       in the anterior clusters of subepidermal cells, which may represent
CC       migrating neural crest cells. By stage 24 (early tailbud stage), the
CC       expression pattern expands to include lateral plate mesoderm in the
CC       mid-embryo, symmetrically along the left-right axis, and the heart
CC       anlage in the ventral midline. By stages 28-30 (late tailbud stages),
CC       the region of expression in the lateral plate mesoderm has expanded
CC       into a symmetrical arc of ventral and lateral expression, which fuses
CC       in the midline where the straight heart tube has formed. At the same
CC       stages, branchial arches present high levels of expression. By stage 33
CC       (beginning of heart looping), expressed in the heart, lateral mesoderm
CC       and branchial arches. The expression persists in the looped heart and
CC       the branchial arches throughout stage 37. Most abundant in the heart
CC       and outflow tract at stage 47 (tadpole stage).
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DR   EMBL; AF228335; AAF67131.1; -; mRNA.
DR   EMBL; AF286645; AAG22008.1; -; mRNA.
DR   EMBL; BC123194; AAI23195.1; -; mRNA.
DR   RefSeq; NP_001079108.1; NM_001085639.1.
DR   AlphaFoldDB; P57101; -.
DR   SMR; P57101; -.
DR   DNASU; 373641; -.
DR   GeneID; 373641; -.
DR   KEGG; xla:373641; -.
DR   CTD; 373641; -.
DR   Xenbase; XB-GENE-865126; hand2.L.
DR   OMA; CHEDSGA; -.
DR   OrthoDB; 1449816at2759; -.
DR   Proteomes; UP000186698; Chromosome 1L.
DR   Bgee; 373641; Expressed in heart and 11 other tissues.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..210
FT                   /note="Heart- and neural crest derivatives-expressed
FT                   protein 2"
FT                   /id="PRO_0000127196"
FT   DOMAIN          92..144
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          81..101
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   210 AA;  23253 MW;  73E3085145489369 CRC64;
     MSLVGGFPHH PVVHHDGYPF AAAAAAAASR CHEENPYFHG WLISHPEMSP PDYSMAPSYS
     PEYANGAAGL DHSHYGGVPG SGAGGLMQRP VKRRGTANRK ERRRTISINS AFAELRECIP
     NVPADTKLSK IKTLRLATSY IAYLMDLLAK DDQNGETEAF KAEIKKTDVK EEKRKKELNE
     LLKSTVCSND KKTKGRTGWP QHVWALELKQ