HAND2_RAT
ID HAND2_RAT Reviewed; 217 AA.
AC P61295; O95300; O95301; P97833;
DT 10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Heart- and neural crest derivatives-expressed protein 2;
DE AltName: Full=Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2;
DE Short=dHAND;
GN Name=Hand2; Synonyms=Dhand;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Wistar; TISSUE=Aorta;
RA Kemp P., Grainger D., Metcalfe J.;
RT "Expression of helix-loop-helix proteins in vascular smooth muscle.";
RL Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Essential for cardiac morphogenesis, particularly for the
CC formation of the right ventricle and of the aortic arch arteries.
CC Required for vascular development and regulation of angiogenesis,
CC possibly through a VEGF signaling pathway. Also plays an important role
CC in limb development, particularly in the establishment of anterior-
CC posterior polarization, acting as an upstream regulator of sonic
CC hedgehog (SHH) induction in the limb bud. Is involved in the
CC development of branchial arches, which give rise to unique structures
CC in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-
CC 3' (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. Forms homodimers and heterodimers with TCF3 gene products E12
CC and E47, HAND1 and HEY1, HEY2 and HEYL (hairy-related transcription
CC factors) (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
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DR EMBL; Y08138; CAA69332.1; -; mRNA.
DR RefSeq; NP_073187.1; NM_022696.1.
DR AlphaFoldDB; P61295; -.
DR SMR; P61295; -.
DR STRING; 10116.ENSRNOP00000033513; -.
DR PaxDb; P61295; -.
DR Ensembl; ENSRNOT00000079552; ENSRNOP00000074299; ENSRNOG00000060448.
DR GeneID; 64637; -.
DR KEGG; rno:64637; -.
DR CTD; 9464; -.
DR RGD; 621207; Hand2.
DR eggNOG; KOG4029; Eukaryota.
DR GeneTree; ENSGT00940000160772; -.
DR InParanoid; P61295; -.
DR OrthoDB; 1449816at2759; -.
DR PhylomeDB; P61295; -.
DR PRO; PR:P61295; -.
DR Proteomes; UP000002494; Chromosome 16.
DR GO; GO:0000785; C:chromatin; ISO:RGD.
DR GO; GO:0005634; C:nucleus; IDA:RGD.
DR GO; GO:0032991; C:protein-containing complex; ISO:RGD.
DR GO; GO:0005667; C:transcription regulator complex; ISO:RGD.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IMP:BHF-UCL.
DR GO; GO:0070888; F:E-box binding; ISO:RGD.
DR GO; GO:0003680; F:minor groove of adenine-thymine-rich DNA binding; ISO:RGD.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:RGD.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; ISO:RGD.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; IPI:BHF-UCL.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:RGD.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:RGD.
DR GO; GO:0001223; F:transcription coactivator binding; IPI:BHF-UCL.
DR GO; GO:0007512; P:adult heart development; ISO:RGD.
DR GO; GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
DR GO; GO:0003278; P:apoptotic process involved in heart morphogenesis; ISO:RGD.
DR GO; GO:0061309; P:cardiac neural crest cell development involved in outflow tract morphogenesis; ISO:RGD.
DR GO; GO:0003253; P:cardiac neural crest cell migration involved in outflow tract morphogenesis; ISO:RGD.
DR GO; GO:0003219; P:cardiac right ventricle formation; ISO:RGD.
DR GO; GO:0060536; P:cartilage morphogenesis; ISO:RGD.
DR GO; GO:0061325; P:cell proliferation involved in outflow tract morphogenesis; ISO:RGD.
DR GO; GO:0071407; P:cellular response to organic cyclic compound; IEP:RGD.
DR GO; GO:0071300; P:cellular response to retinoic acid; IEP:RGD.
DR GO; GO:0060982; P:coronary artery morphogenesis; ISO:RGD.
DR GO; GO:0061371; P:determination of heart left/right asymmetry; ISO:RGD.
DR GO; GO:0032502; P:developmental process; IBA:GO_Central.
DR GO; GO:0042733; P:embryonic digit morphogenesis; ISO:RGD.
DR GO; GO:0048706; P:embryonic skeletal system development; ISO:RGD.
DR GO; GO:1904019; P:epithelial cell apoptotic process; IEA:Ensembl.
DR GO; GO:0007507; P:heart development; IEP:UniProtKB.
DR GO; GO:0001947; P:heart looping; ISO:RGD.
DR GO; GO:0003007; P:heart morphogenesis; ISO:RGD.
DR GO; GO:0001701; P:in utero embryonic development; ISO:RGD.
DR GO; GO:0010463; P:mesenchymal cell proliferation; ISO:RGD.
DR GO; GO:0060485; P:mesenchyme development; ISO:RGD.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:RGD.
DR GO; GO:0010667; P:negative regulation of cardiac muscle cell apoptotic process; ISO:RGD.
DR GO; GO:0043392; P:negative regulation of DNA binding; ISO:RGD.
DR GO; GO:0043433; P:negative regulation of DNA-binding transcription factor activity; ISO:RGD.
DR GO; GO:1904036; P:negative regulation of epithelial cell apoptotic process; IEA:Ensembl.
DR GO; GO:0010629; P:negative regulation of gene expression; ISO:RGD.
DR GO; GO:0045668; P:negative regulation of osteoblast differentiation; ISO:RGD.
DR GO; GO:0014032; P:neural crest cell development; ISO:RGD.
DR GO; GO:0042475; P:odontogenesis of dentin-containing tooth; ISO:RGD.
DR GO; GO:0001649; P:osteoblast differentiation; IEA:Ensembl.
DR GO; GO:0048935; P:peripheral nervous system neuron development; ISO:RGD.
DR GO; GO:0010613; P:positive regulation of cardiac muscle hypertrophy; ISO:RGD.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; ISO:RGD.
DR GO; GO:0010628; P:positive regulation of gene expression; ISO:RGD.
DR GO; GO:1900745; P:positive regulation of p38MAPK cascade; ISO:RGD.
DR GO; GO:2000764; P:positive regulation of semaphorin-plexin signaling pathway involved in outflow tract morphogenesis; ISO:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:2000763; P:positive regulation of transcription from RNA polymerase II promoter involved in norepinephrine biosynthetic process; ISO:RGD.
DR GO; GO:2000679; P:positive regulation of transcription regulatory region DNA binding; ISO:RGD.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:1903929; P:primary palate development; ISO:RGD.
DR GO; GO:0003266; P:regulation of secondary heart field cardioblast proliferation; ISO:RGD.
DR GO; GO:0034103; P:regulation of tissue remodeling; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0060021; P:roof of mouth development; ISO:RGD.
DR GO; GO:0001967; P:suckling behavior; ISO:RGD.
DR GO; GO:0048485; P:sympathetic nervous system development; ISO:RGD.
DR GO; GO:0048538; P:thymus development; ISO:RGD.
DR GO; GO:0043586; P:tongue development; ISO:RGD.
DR GO; GO:0061032; P:visceral serous pericardium development; ISO:RGD.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Angiogenesis; Developmental protein; Differentiation; DNA-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..217
FT /note="Heart- and neural crest derivatives-expressed
FT protein 2"
FT /id="PRO_0000127193"
FT DOMAIN 99..151
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 76..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 217 AA; 23666 MW; 528F541BB2173F1E CRC64;
MSLVGGFPHH PVVHHEGYPF AAAAAAAAAA AASRCSHEEN PYFHGWLIGH PEMSPPDYSM
ALSYSPEYAS GAAGLDHSHY GGVPPGAGPP GLGGPRPVKR RGTANRKERR RTQSINSAFA
ELRECIPNVP ADTKLSKIKT LRLATSYIAY LMDLLAKDDQ NGEAEAFKAE IKKTDVKEEK
RKKELNEILK STVSSNDKKT KGRTGWPQHV WALELKQ
