ID   HAND2_CHICK             Reviewed;         216 AA.
AC   Q90690;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Heart- and neural crest derivatives-expressed protein 2;
DE   AltName: Full=Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2;
DE            Short=dHAND;
GN   Name=HAND2; Synonyms=DHAND;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Embryo;
RX   PubMed=8533092; DOI=10.1126/science.270.5244.1995;
RA   Srivastava D., Cserjesi P., Olson E.N.;
RT   "A subclass of bHLH proteins required for cardiac morphogenesis.";
RL   Science 270:1995-1998(1995).
RN   [2]
RP   FUNCTION, AND DEVELOPMENTAL STAGE.
RX   PubMed=10769237; DOI=10.1242/dev.127.10.2133;
RA   Fernandez-Teran M., Piedra M.E., Kathiriya I.S., Srivastava D.,
RA   Rodriguez-Rey J.C., Ros M.A.;
RT   "Role of dHAND in the anterior-posterior polarization of the limb bud:
RT   implications for the Sonic hedgehog pathway.";
RL   Development 127:2133-2142(2000).
CC   -!- FUNCTION: Essential for cardiac morphogenesis. Binds DNA on E-box
CC       consensus sequence 5'-CANNTG-3' (By similarity). Plays an important
CC       role in limb development, particularly in the establishment of
CC       anterior-posterior polarization of the limb bud. {ECO:0000250,
CC       ECO:0000269|PubMed:10769237}.
CC   -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC       protein.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC   -!- DEVELOPMENTAL STAGE: At stages 8 to 9, expressed in the whole lateral
CC       plate mesoderm and precardiogenic mesoderm. At stage 10, expressed
CC       throughout the cardiac tube and the sinus venosus. By stage 15,
CC       expressed homogeneously in the various region of the heart, including
CC       the atria, future left ventricle, bulbus cordis and truncus arteriosus,
CC       and in the forming branchial arches. Expression persists through stage
CC       20, but decreases thereafter. In the developing limbs, from the
CC       initiation of the buds (stages 16 to 17), expression is down-regulated
CC       at the anterior of the limb buds so that a gradient expression along
CC       the anterior-posterior axis of the bud is established with higher
CC       expression at the posterior border. At later stages, expression is
CC       restricted to the posterior border of the zeugopod and to the posterior
CC       autopod. In the autopod, dynamic expression of HAND2 affects the
CC       interdigital regions, the lateral borders of the digits and eventually
CC       the developing ventral tendons. {ECO:0000269|PubMed:10769237}.
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DR   EMBL; U40040; AAC59733.1; -; mRNA.
DR   AlphaFoldDB; Q90690; -.
DR   SMR; Q90690; -.
DR   VEuPathDB; HostDB:geneid_395813; -.
DR   InParanoid; Q90690; -.
DR   PhylomeDB; Q90690; -.
DR   PRO; PR:Q90690; -.
DR   Proteomes; UP000000539; Unplaced.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0032502; P:developmental process; IBA:GO_Central.
DR   GO; GO:0007507; P:heart development; IBA:GO_Central.
DR   GO; GO:0060174; P:limb bud formation; IMP:AgBase.
DR   GO; GO:0009949; P:polarity specification of anterior/posterior axis; IMP:AgBase.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..216
FT                   /note="Heart- and neural crest derivatives-expressed
FT                   protein 2"
FT                   /id="PRO_0000127195"
FT   DOMAIN          98..150
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          74..115
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   216 AA;  24407 MW;  0B3F052F2CC34C6B CRC64;
     MSLVGGFPHH PVVHHEGYPL RRRRCRRRRR HPLRPRGEPL LHGWLISSHP EMSPPDYSMA
     LSYSPEYANG APGMDHSHYG GVPPGSGPPG LGGPRPVKRR GTANRKERRR TQSINSAFAE
     LRECIPNVPA DTKLSKIKTL RLATSYIAYL MDLLPKDDQN GEAEAFKAEI KKTDVKEEKR
     KKELNEILKS TVSSSDKKTK GRTGWPQHVW ALELKQ