HAND1_RAT
ID HAND1_RAT Reviewed; 216 AA.
AC P97832; Q4V8L8;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Heart- and neural crest derivatives-expressed protein 1;
DE AltName: Full=Extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1;
DE Short=eHAND;
GN Name=Hand1; Synonyms=Ehand;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Wistar; TISSUE=Aorta;
RA Kemp P., Grainger D., Metcalfe J.;
RT "Expression of helix-loop-helix proteins in vascular smooth muscle.";
RL Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Transcription factor that plays an essential role in both
CC trophoblast giant cell differentiation and in cardiac morphogenesis (By
CC similarity). Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box)
CC (By similarity). Acts as a transcriptional repressor of SOX15 (By
CC similarity). In the adult, could be required for ongoing expression of
CC cardiac-specific genes (By similarity). {ECO:0000250|UniProtKB:O96004,
CC ECO:0000250|UniProtKB:Q64279}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. Forms homodimers and heterodimers with TCF3 gene products E12
CC and E47, HAND2 and HEY1, HEY2 and HEYL (hairy-related transcription
CC factors). Interacts with MDFIC (By similarity). Interacts with SOX15;
CC the interaction enhances HAND1-induced differentiation of trophoblast
CC giant cells (By similarity). {ECO:0000250,
CC ECO:0000250|UniProtKB:Q64279}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleoplasm {ECO:0000250}. Nucleus,
CC nucleolus {ECO:0000250}. Note=Interaction with MDFIC sequesters it into
CC the nucleolus, preventing the transcription factor activity.
CC Phosphorylation by PLK4 disrupts the interaction with MDFIC and
CC releases it from the nucleolus, leading to transcription factor
CC activity (By similarity). {ECO:0000250}.
CC -!- PTM: Phosphorylation by PLK4 disrupts the interaction with MDFIC and
CC leads to translocation into the nucleoplasm, allowing dimerization and
CC transcription factor activity. {ECO:0000250}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; Y08140; CAA69334.1; -; mRNA.
DR EMBL; BC097324; AAH97324.1; -; mRNA.
DR RefSeq; NP_067603.1; NM_021592.2.
DR AlphaFoldDB; P97832; -.
DR SMR; P97832; -.
DR STRING; 10116.ENSRNOP00000003486; -.
DR iPTMnet; P97832; -.
DR PaxDb; P97832; -.
DR Ensembl; ENSRNOT00000003486; ENSRNOP00000003486; ENSRNOG00000002582.
DR GeneID; 59112; -.
DR KEGG; rno:59112; -.
DR UCSC; RGD:621206; rat.
DR CTD; 9421; -.
DR RGD; 621206; Hand1.
DR eggNOG; KOG4029; Eukaryota.
DR GeneTree; ENSGT00940000161111; -.
DR HOGENOM; CLU_119591_0_0_1; -.
DR InParanoid; P97832; -.
DR OMA; YFQSWVL; -.
DR OrthoDB; 1449816at2759; -.
DR PhylomeDB; P97832; -.
DR TreeFam; TF315153; -.
DR PRO; PR:P97832; -.
DR Proteomes; UP000002494; Chromosome 10.
DR Bgee; ENSRNOG00000002582; Expressed in colon and 5 other tissues.
DR Genevisible; P97832; RN.
DR GO; GO:0005737; C:cytoplasm; ISO:RGD.
DR GO; GO:0005730; C:nucleolus; ISO:RGD.
DR GO; GO:0005654; C:nucleoplasm; ISO:RGD.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; ISO:RGD.
DR GO; GO:0043425; F:bHLH transcription factor binding; ISO:RGD.
DR GO; GO:0003677; F:DNA binding; ISO:RGD.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:RGD.
DR GO; GO:0019899; F:enzyme binding; ISO:RGD.
DR GO; GO:0042802; F:identical protein binding; ISO:RGD.
DR GO; GO:0042803; F:protein homodimerization activity; ISO:RGD.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:RGD.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0061629; F:RNA polymerase II-specific DNA-binding transcription factor binding; ISO:RGD.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:RGD.
DR GO; GO:0001221; F:transcription coregulator binding; ISO:RGD.
DR GO; GO:0001525; P:angiogenesis; ISO:RGD.
DR GO; GO:0001824; P:blastocyst development; ISO:RGD.
DR GO; GO:0003218; P:cardiac left ventricle formation; ISO:RGD.
DR GO; GO:0003219; P:cardiac right ventricle formation; ISO:RGD.
DR GO; GO:0060411; P:cardiac septum morphogenesis; ISO:RGD.
DR GO; GO:0060536; P:cartilage morphogenesis; ISO:RGD.
DR GO; GO:0030154; P:cell differentiation; ISO:RGD.
DR GO; GO:0061371; P:determination of heart left/right asymmetry; ISO:RGD.
DR GO; GO:0032502; P:developmental process; IBA:GO_Central.
DR GO; GO:0035050; P:embryonic heart tube development; ISO:RGD.
DR GO; GO:0003144; P:embryonic heart tube formation; ISO:RGD.
DR GO; GO:0007507; P:heart development; ISO:RGD.
DR GO; GO:0001947; P:heart looping; ISO:RGD.
DR GO; GO:0001701; P:in utero embryonic development; ISO:RGD.
DR GO; GO:0060485; P:mesenchyme development; ISO:RGD.
DR GO; GO:0001707; P:mesoderm formation; ISO:RGD.
DR GO; GO:0043433; P:negative regulation of DNA-binding transcription factor activity; ISO:RGD.
DR GO; GO:1903026; P:negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding; ISO:RGD.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISO:RGD.
DR GO; GO:0042475; P:odontogenesis of dentin-containing tooth; ISO:RGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:RGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0001829; P:trophectodermal cell differentiation; ISO:RGD.
DR GO; GO:0060707; P:trophoblast giant cell differentiation; ISS:UniProtKB.
DR GO; GO:0055010; P:ventricular cardiac muscle tissue morphogenesis; ISO:RGD.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Activator; Developmental protein; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..216
FT /note="Heart- and neural crest derivatives-expressed
FT protein 1"
FT /id="PRO_0000127187"
FT DOMAIN 94..146
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 73..109
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 165..203
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 165..184
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 107
FT /note="Phosphothreonine; by PLK4"
FT /evidence="ECO:0000250|UniProtKB:Q64279"
FT MOD_RES 109
FT /note="Phosphoserine; by PLK4"
FT /evidence="ECO:0000250|UniProtKB:Q64279"
SQ SEQUENCE 216 AA; 23810 MW; FD047598350C9BBC CRC64;
MNLVGSYAHH HHHHHSHPPH PMLHEPFLFG PASRCHQERP YFQSWLLSPA DAAPDFPAGG
PPPTTAVAAA AYGPDARPSQ SPGRLEALGG RLPRRKGSGP KKERRRTESI NSAFAELREC
IPNVPADTKL SKIKTLRLAT SYIAYLMDVL AKDAQAGDPE AFKAELKKTD GGRESKRKRE
SPQQHEGLPP ALGPSEKRIK GRTGWPQQVW ALELNQ
