HAM_DROME
ID HAM_DROME Reviewed; 990 AA.
AC Q8I7Z8; Q9VJ50; Q9VJ52;
DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Transcription factor hamlet;
GN Name=ham; ORFNames=CG31753;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RC TISSUE=Embryo;
RX PubMed=12193790; DOI=10.1126/science.1072387;
RA Moore A.W., Jan L.Y., Jan Y.-N.;
RT "Hamlet, a binary genetic switch between single- and multiple-dendrite
RT neuron morphology.";
RL Science 297:1355-1358(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=15075290; DOI=10.1101/gad.1170904;
RA Moore A.W., Roegiers F., Jan L.Y., Jan Y.-N.;
RT "Conversion of neurons and glia to external-cell fates in the external
RT sensory organs of Drosophila hamlet mutants by a cousin-cousin cell-type
RT respecification.";
RL Genes Dev. 18:623-628(2004).
CC -!- FUNCTION: Transcription factor that plays a crucial role in external
CC sensory organ (ESO) that elaborates from a single precursor cell (ESOP
CC cell). Mediates the differentiation of lineage branch that generates
CC the internal 'ES' and 'th' cells (external sensory neuron and thecogen
CC cells, respectively) from the IIIB cell. Its absence leads to re-
CC specification of IIIB daughter cells into external tormagen cells (tr
CC cells), or internal multidendritic neurons (MD cells). One of its role
CC in the development of the ESO lineage is to modulate the activity of
CC Notch and PAX2 signals. {ECO:0000269|PubMed:12193790,
CC ECO:0000269|PubMed:15075290}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12193790}.
CC -!- TISSUE SPECIFICITY: Expressed in the developing peripheral nervous
CC system (PNS). Expressed in the ventral patch in the cephalic region of
CC the embryo from stage 5, and continues in cephalic region through stage
CC 15. Expressed in the developing PNS from stage 11 to 15, with a
CC transient expression in each PNS cluster. In the ESOP lineage, it is
CC first expressed in the IIIB cell, and in both ES and th cells, although
CC it is only transiently expressed in th cells. In the ESOP lineage in
CC the adult, it is also expressed in the IIIB cell and inherited by its
CC daughter cells, as in the embryo. {ECO:0000269|PubMed:12193790,
CC ECO:0000269|PubMed:15075290}.
CC -!- MISCELLANEOUS: Was named 'hamlet' because of its 'to be or not to be'
CC role in ESOP cells differentiation.
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DR EMBL; AY166585; AAN86032.1; -; mRNA.
DR EMBL; AE014134; AAF53703.4; -; Genomic_DNA.
DR RefSeq; NP_001246081.1; NM_001259152.1.
DR RefSeq; NP_001260543.1; NM_001273614.1.
DR RefSeq; NP_724130.3; NM_165262.3.
DR AlphaFoldDB; Q8I7Z8; -.
DR BioGRID; 61129; 4.
DR IntAct; Q8I7Z8; 1.
DR STRING; 7227.FBpp0304333; -.
DR PaxDb; Q8I7Z8; -.
DR PRIDE; Q8I7Z8; -.
DR EnsemblMetazoa; FBtr0305620; FBpp0294066; FBgn0045852.
DR EnsemblMetazoa; FBtr0332015; FBpp0304333; FBgn0045852.
DR GeneID; 35135; -.
DR KEGG; dme:Dmel_CG31753; -.
DR UCSC; CG31753-RA; d. melanogaster.
DR CTD; 35135; -.
DR FlyBase; FBgn0045852; ham.
DR VEuPathDB; VectorBase:FBgn0045852; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000157208; -.
DR InParanoid; Q8I7Z8; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q8I7Z8; -.
DR Reactome; R-DME-8943724; Regulation of PTEN gene transcription.
DR BioGRID-ORCS; 35135; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 35135; -.
DR PRO; PR:Q8I7Z8; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0045852; Expressed in presumptive embryonic/larval peripheral nervous system (Drosophila) and 27 other tissues.
DR ExpressionAtlas; Q8I7Z8; baseline and differential.
DR Genevisible; Q8I7Z8; DM.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:UniProtKB.
DR GO; GO:0046974; F:histone methyltransferase activity (H3-K9 specific); ISS:FlyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0048813; P:dendrite morphogenesis; TAS:FlyBase.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IMP:FlyBase.
DR GO; GO:0014017; P:neuroblast fate commitment; IMP:FlyBase.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0050773; P:regulation of dendrite development; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR Gene3D; 2.170.270.10; -; 1.
DR InterPro; IPR046341; SET_dom_sf.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 6.
DR SMART; SM00355; ZnF_C2H2; 9.
DR SUPFAM; SSF57667; SSF57667; 5.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 8.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 8.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; DNA-binding; Metal-binding;
KW Notch signaling pathway; Nucleus; Reference proteome; Repeat;
KW Transcription; Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..990
FT /note="Transcription factor hamlet"
FT /id="PRO_0000046941"
FT ZN_FING 221..245
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 251..274
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 280..303
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 309..331
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 337..359
FT /note="C2H2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 366..388
FT /note="C2H2-type 6; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 817..839
FT /note="C2H2-type 7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 845..868
FT /note="C2H2-type 8"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 874..896
FT /note="C2H2-type 9"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 164..196
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 392..440
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 554..633
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 667..725
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 892..913
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 933..990
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 178..196
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 419..440
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 554..612
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 676..699
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 711..725
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 990 AA; 109228 MW; 3CF038543CA63717 CRC64;
MDAFFKDRAQ AEHILQEWVR RREPVCELDI RDSGGVYAKT PLQRGTRYGP FPMKLSHQPN
DPQLAWKAHS RHYNGWLEPT EDVSTWLKKI RSVQDDCIGE ANLQSYINAG YLWYETNRYV
NAGSEMVVDG RPKSPVQLNE DFMNGGKVLA AAAAAAAAVV AASSSGAKSG GGGSSAPSDD
RSDRDNGSLY SGDEFSKDKK NSSLIREGDI DFTDDENGFD IRCEVCDKVY PDLDLLDDHL
IGAHHFKQDE FPCKQCALRF CHRPLLIKHE AISHNNIRKY SCENCSKVFC DPSNLQRHIR
AYHVGARCHP CPECGKTFGT SSGLKQHQHI HSSVKPFACE VCSKAYTQFS NLCRHKRMHA
TCRMQIKCDK CNQSFSTLTS LTKHKKFCDS TGPGPYRNQH VNRHHQHPHQ HPLPHQPHLA
ATATSTCPAP PRESSESSSS AAAAAVAAMS TPPNPFLMFR TAPSFFPGFP PYGFPPFLPQ
NPLHPTNIPM FFSKNPMDLG CGGPEITSPV SAFDQKLPFG FLKGENSESQ AYDKVTEKEL
VFKAEEKLKK EPLVQAFEGE EDESRSSLDI KGKLEDTRND SKSEEQDDMK QEPERVSTPD
QQQAEDDRKS IDIMSTPPPA DTPSGGDGPL DLSICRKRSA GSFFTAPAED NLMLHRFMPR
LHEFEAERGQ PLKMRKSHSS AESSTSQKSH KGSSPTPTPT ASPGLTPSPS PPTSAGGELS
STSEGGAVPT MAAAAFAADH SALASGPTLP QTHPTFHPLL LEEIYRSGFP FLCQPGGRRG
IEALLAGAAS AAAPKRPLPP VKFSAGSVVG LKTKDRYSCK FCGKVFPRSA NLTRHLRTHT
GEQPYPCKYC DRAFSISSNL QRHVRNIHNK ERPFRCELCD RSFGQQTNLD RHVKKHESEG
NNFRDSPSSS GIAEREEYFD DIRKFMNRVY TPNSLAGNEG DTEEYPNSDD QSVNLEKDSG
NFNNNSSNIS NNNSSSGNNN NSSKAITISS
