HAM1_CAEEL
ID HAM1_CAEEL Reviewed; 414 AA.
AC G5EEC5;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=Stork-head box protein ham-1 {ECO:0000305};
DE AltName: Full=HSN abnormal migration 1 {ECO:0000312|WormBase:F53B2.6};
GN Name=ham-1 {ECO:0000312|WormBase:F53B2.6};
GN ORFNames=F53B2.6 {ECO:0000312|WormBase:F53B2.6};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, AND MUTAGENESIS OF GLY-47.
RX PubMed=3200316; DOI=10.1038/336638a0;
RA Desai C., Garriga G., McIntire S.L., Horvitz H.R.;
RT "A genetic pathway for the development of the Caenorhabditis elegans HSN
RT motor neurons.";
RL Nature 336:638-646(1988).
RN [3] {ECO:0000305}
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=8951066; DOI=10.1242/dev.122.11.3509;
RA Guenther C., Garriga G.;
RT "Asymmetric distribution of the C. elegans HAM-1 protein in neuroblasts
RT enables daughter cells to adopt distinct fates.";
RL Development 122:3509-3518(1996).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF GLY-47 AND
RP 62-GLN--LEU-414.
RX PubMed=15979607; DOI=10.1016/j.ydbio.2005.05.026;
RA Frank C.A., Hawkins N.C., Guenther C., Horvitz H.R., Garriga G.;
RT "C. elegans HAM-1 positions the cleavage plane and regulates apoptosis in
RT asymmetric neuroblast divisions.";
RL Dev. Biol. 284:301-310(2005).
RN [5] {ECO:0000305}
RP FUNCTION.
RX PubMed=18505863; DOI=10.1534/genetics.108.088948;
RA Singhvi A., Frank C.A., Garriga G.;
RT "The T-box gene tbx-2, the homeobox gene egl-5 and the asymmetric cell
RT division gene ham-1 specify neural fate in the HSN/PHB lineage.";
RL Genetics 179:887-898(2008).
RN [6] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=23946438; DOI=10.1242/dev.098723;
RA Feng G., Yi P., Yang Y., Chai Y., Tian D., Zhu Z., Liu J., Zhou F.,
RA Cheng Z., Wang X., Li W., Ou G.;
RT "Developmental stage-dependent transcriptional regulatory pathways control
RT neuroblast lineage progression.";
RL Development 140:3838-3847(2013).
RN [7] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF 1-MET--PHE-31;
RP 251-PRO--PRO-261; 286-LYS--ARG-290 AND 321-PRO--ARG-327.
RX PubMed=26703426; DOI=10.1016/j.ydbio.2015.12.011;
RA Leung A., Hua K., Ramachandran P., Hingwing K., Wu M., Koh P.L.,
RA Hawkins N.;
RT "C. elegans HAM-1 functions in the nucleus to regulate asymmetric
RT neuroblast division.";
RL Dev. Biol. 410:56-69(2016).
RN [8] {ECO:0000305}
RP FUNCTION.
RX PubMed=28659600; DOI=10.1038/s41598-017-04375-4;
RA Offenburger S.L., Bensaddek D., Murillo A.B., Lamond A.I., Gartner A.;
RT "Comparative genetic, proteomic and phosphoproteomic analysis of C. elegans
RT embryos with a focus on ham-1/STOX and pig-1/MELK in dopaminergic neuron
RT development.";
RL Sci. Rep. 7:4314-4314(2017).
RN [9] {ECO:0000305}
RP FUNCTION, AND MUTAGENESIS OF 62-GLN--LEU-414.
RX PubMed=29668718; DOI=10.1371/journal.pone.0195855;
RA Teuliere J., Kovacevic I., Bao Z., Garriga G.;
RT "The Caenorhabditis elegans gene ham-1 regulates daughter cell size
RT asymmetry primarily in divisions that produce a small anterior daughter
RT cell.";
RL PLoS ONE 13:e0195855-e0195855(2018).
CC -!- FUNCTION: Probable transcription factor (PubMed:23946438). Required for
CC asymmetric cell division in neuroblasts, perhaps acting by regulating
CC spindle positioning and myosin polarization, and thus the position of
CC the cleavage plane (PubMed:3200316, PubMed:18505863, PubMed:8951066,
CC PubMed:26703426, PubMed:15979607, PubMed:23946438). Required to produce
CC daughter cell size asymmetry in neuroblasts undergoing asymmetric cell
CC division, usually giving rise to one precursor cell and one apoptotic
CC cell (PubMed:29668718, PubMed:15979607, PubMed:23946438). Positively
CC modulates expression of the serine/threonine kinase pig-1/MELK during
CC asymmetric division of the Q.a neuroblast (PubMed:23946438). Plays a
CC role in neural fate specification in several dopaminergic lineages,
CC including the hermaphrodite-specific neuron (HSN)/phasmid neuron (PHB),
CC acting in concert with the kinase, ham-1, and the T-box protein tbx-2
CC and the homeobox protein egl-5 (PubMed:3200316, PubMed:18505863,
CC PubMed:8951066, PubMed:28659600, PubMed:23946438).
CC {ECO:0000269|PubMed:15979607, ECO:0000269|PubMed:18505863,
CC ECO:0000269|PubMed:23946438, ECO:0000269|PubMed:26703426,
CC ECO:0000269|PubMed:28659600, ECO:0000269|PubMed:29668718,
CC ECO:0000269|PubMed:3200316, ECO:0000269|PubMed:8951066}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cell cortex
CC {ECO:0000269|PubMed:15979607, ECO:0000269|PubMed:26703426}. Nucleus
CC {ECO:0000269|PubMed:23946438, ECO:0000269|PubMed:26703426}. Cytoplasm
CC {ECO:0000269|PubMed:23946438, ECO:0000269|PubMed:26703426}.
CC Note=Distributed asymmetrically in the cell cortex in some embryonic
CC cells, including the hermaphrodite-specific neuron (HSN)/phasmid neuron
CC (PHB) neuroblast (PubMed:15979607, PubMed:26703426). Localized to
CC nucleus during interphase and evenly distributed in the cytoplasm of
CC dividing Q.a and Q.p neuroblasts (PubMed:23946438).
CC {ECO:0000269|PubMed:15979607, ECO:0000269|PubMed:23946438,
CC ECO:0000269|PubMed:26703426}.
CC -!- DEVELOPMENTAL STAGE: First expressed at the onset of gastrulation,
CC approximately 100 minutes after cleavage of the zygote (at protein
CC level) (PubMed:8951066). Expressed in both Q.a and Q.p neuroblasts as
CC well as in their neighboring cells (PubMed:23946438).
CC {ECO:0000269|PubMed:23946438, ECO:0000269|PubMed:8951066}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BX284604; CAA98132.1; -; Genomic_DNA.
DR PIR; T22539; T22539.
DR RefSeq; NP_502401.1; NM_070000.6.
DR AlphaFoldDB; G5EEC5; -.
DR SMR; G5EEC5; -.
DR STRING; 6239.F53B2.6; -.
DR EPD; G5EEC5; -.
DR PaxDb; G5EEC5; -.
DR EnsemblMetazoa; F53B2.6.1; F53B2.6.1; WBGene00001820.
DR GeneID; 178205; -.
DR KEGG; cel:CELE_F53B2.6; -.
DR CTD; 178205; -.
DR WormBase; F53B2.6; CE17851; WBGene00001820; ham-1.
DR eggNOG; KOG3897; Eukaryota.
DR GeneTree; ENSGT00520000055589; -.
DR HOGENOM; CLU_736153_0_0_1; -.
DR InParanoid; G5EEC5; -.
DR OMA; GPIDPPE; -.
DR OrthoDB; 970701at2759; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00001820; Expressed in embryo and 3 other tissues.
DR GO; GO:0005938; C:cell cortex; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IMP:WormBase.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IMP:UniProtKB.
DR GO; GO:0055059; P:asymmetric neuroblast division; IMP:UniProtKB.
DR GO; GO:0045167; P:asymmetric protein localization involved in cell fate determination; IMP:WormBase.
DR GO; GO:0001709; P:cell fate determination; IMP:WormBase.
DR GO; GO:0071542; P:dopaminergic neuron differentiation; IMP:UniProtKB.
DR GO; GO:0051402; P:neuron apoptotic process; IMP:UniProtKB.
DR GO; GO:0001764; P:neuron migration; IMP:WormBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:1990179; P:protein localization to actomyosin contractile ring; IMP:UniProtKB.
DR GO; GO:0008361; P:regulation of cell size; IMP:UniProtKB.
DR GO; GO:0051653; P:spindle localization; IMP:UniProtKB.
DR InterPro; IPR019391; Storkhead-box_winged-helix.
DR InterPro; IPR040126; STOX1/2.
DR PANTHER; PTHR22437; PTHR22437; 2.
DR Pfam; PF10264; Stork_head; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome.
FT CHAIN 1..414
FT /note="Stork-head box protein ham-1"
FT /id="PRO_0000454717"
FT DOMAIN 93..170
FT /note="Winged helix Storkhead-box1"
FT /evidence="ECO:0000255"
FT REGION 1..31
FT /note="Essential for association with cell cortex"
FT /evidence="ECO:0000269|PubMed:26703426"
FT REGION 282..362
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 285..295
FT /note="Bi-partite nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:26703426"
FT REGION 321..327
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:26703426"
FT COMPBIAS 329..362
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 1..31
FT /note="Missing: Almost completely eliminates localization
FT to cell cortex."
FT /evidence="ECO:0000269|PubMed:26703426"
FT MUTAGEN 47
FT /note="G->D: In n1810; defects in the hermaphrodite-
FT specific neurons (HSN); abnormal migration, reduced levels
FT of serotonin, also egg-laying defects. Localized
FT inappropriately to the cytoplasm."
FT /evidence="ECO:0000269|PubMed:15979607,
FT ECO:0000269|PubMed:3200316"
FT MUTAGEN 62..414
FT /note="Missing: In gm279; embryos have no detectable ham-1
FT protein. Some daughter cells of neuroblasts which would
FT normally undergo programmed cell death have abnormally
FT large corpses. Daughter cell size asymmetry is reduced or
FT lost."
FT /evidence="ECO:0000269|PubMed:15979607,
FT ECO:0000269|PubMed:29668718"
FT MUTAGEN 251..261
FT /note="Missing: Significantly reduces localization to the
FT cell cortex."
FT /evidence="ECO:0000269|PubMed:26703426"
FT MUTAGEN 286..290
FT /note="KARRR->AAAAA: Modest reduction in localization to
FT nucleus. Results in localization exclusively to the cell
FT cortex with little or no nuclear localization; when
FT associated with 321-P--R-327 del."
FT /evidence="ECO:0000269|PubMed:26703426"
FT MUTAGEN 321..327
FT /note="Missing: Modest reduction in localization to
FT nucleus. Results in localization exclusively to the cell
FT cortex with little or no nuclear localization; when
FT associated with 286-K--R-290."
FT /evidence="ECO:0000269|PubMed:26703426"
SQ SEQUENCE 414 AA; 46169 MW; 89A7F6760FBEA87D CRC64;
MTYLAVVLNG PKAKNGRKVF DSFLEQNRQM FWNRELTSAC ESITYMGFMR PGTLFVSGPA
SQLTVLKDAW ARRILKPAMG YTITSLGDLG AIQQVEQMHF VPLGDVICDA VAQLNRQGLA
ATEQAIRQYV ARHCPHVAPP GIEMVRQTIT SLLSTGFVYK MADHYFVSVP TNSPMRPPAK
AATKTTKTTV ECQTGASMMC PQQTSTSSDE HVIEPAKKDH KKCQNSNRRS IFARLFSRGM
KPQTIMPSAS PIHVGGPPTP PPAAMLPTKN KYPTYHHDLN EECQRKARRR NHPRRGETQK
LLSSSSECLK YYPVDMPETR PTRRRARMAS PLRSSTPNNS DSAYSISPPH TDSNEEAGSI
SDSEINHTYI NINKFRRQNF DSTQFEDLTG ATATTSEEPE ILGRHIRGVL ISNL
