HAL9_YEAST
ID HAL9_YEAST Reviewed; 1030 AA.
AC Q12180; D6W1X9;
DT 18-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Halotolerance protein 9;
GN Name=HAL9; OrderedLocusNames=YOL089C; ORFNames=O0938;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8533473; DOI=10.1002/yea.320111009;
RA Zumstein E., Pearson B.M., Kalogeropoulos A., Schweizer M.;
RT "A 29.425 kb segment on the left arm of yeast chromosome XV contains more
RT than twice as many unknown as known open reading frames.";
RL Yeast 11:975-986(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION.
RX PubMed=9559673; DOI=10.1016/s0014-5793(98)00249-x;
RA Mendizabal I., Rios G., Mulet J.M., Serrano R., de Larrinoa I.F.;
RT "Yeast putative transcription factors involved in salt tolerance.";
RL FEBS Lett. 425:323-328(1998).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-937, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-221, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Putative transcription factor involved in halotolerance.
CC {ECO:0000269|PubMed:9559673}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC {ECO:0000255|PROSITE-ProRule:PRU00227, ECO:0000269|PubMed:14562095}.
CC -!- MISCELLANEOUS: Present with 238 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; X83121; CAA58190.1; -; Genomic_DNA.
DR EMBL; Z74831; CAA99101.1; -; Genomic_DNA.
DR EMBL; BK006948; DAA10695.1; -; Genomic_DNA.
DR PIR; S57380; S57380.
DR RefSeq; NP_014552.1; NM_001183343.1.
DR AlphaFoldDB; Q12180; -.
DR SMR; Q12180; -.
DR BioGRID; 34313; 109.
DR IntAct; Q12180; 17.
DR MINT; Q12180; -.
DR STRING; 4932.YOL089C; -.
DR iPTMnet; Q12180; -.
DR MaxQB; Q12180; -.
DR PaxDb; Q12180; -.
DR PRIDE; Q12180; -.
DR EnsemblFungi; YOL089C_mRNA; YOL089C; YOL089C.
DR GeneID; 854064; -.
DR KEGG; sce:YOL089C; -.
DR SGD; S000005449; HAL9.
DR VEuPathDB; FungiDB:YOL089C; -.
DR eggNOG; ENOG502QZJZ; Eukaryota.
DR GeneTree; ENSGT00940000176304; -.
DR HOGENOM; CLU_008153_0_0_1; -.
DR InParanoid; Q12180; -.
DR OMA; DNMARFE; -.
DR BioCyc; YEAST:G3O-33489-MON; -.
DR PRO; PR:Q12180; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q12180; protein.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005634; C:nucleus; IC:SGD.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; HDA:SGD.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:SGD.
DR GO; GO:0009651; P:response to salt stress; IMP:SGD.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR007219; Transcription_factor_dom_fun.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF04082; Fungal_trans; 1.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00906; Fungal_trans; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; DNA-binding; Metal-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation; Zinc.
FT CHAIN 1..1030
FT /note="Halotolerance protein 9"
FT /id="PRO_0000233012"
FT DNA_BIND 136..166
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 185..208
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 189..208
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 221
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 937
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
SQ SEQUENCE 1030 AA; 117927 MW; 8C8BDE84066D2105 CRC64;
MENQGGDYSP NGFSNSASNM NAVFNNEITG RSDISNVNHQ TGTPRLVPET QIWSMPVPDQ
LMTMPNRENT LMTGSTIGPN IPMNVAYPNT IYSPTEHQSQ FQTQQNRDIS TMMEHTNSND
MSGSGKNLKK RVSKACDHCR KRKIRCDEVD QQTKKCSNCI KFQLPCTFKH RDEILKKKRK
LEIKHHATPG ESLQTSNSIS NPVASSSVPN SGRFELLNGN SPLESNIIDK VSNIQNNLNK
KMNSKIEKLD RKMSYIIDSV ARLEWLLDKA VKKQEGKYKE KNNLPKPARK IYSTALLTAQ
KLYWFKQSLG VKASNEEFLS PISEILSISL KWYATQMKKF MDLSSPAFFS SEIILYSLPP
KKQAKRLLEN FHATLLSSVT GIISLKECLD LAEKYYSESG EKLTYPEHLL LNVCLCSGAS
ATQSIIRGDS KFLRKDRYDP TSQELKKIEN VALLNAMYYY HKLSTICSGT RTLQALLLLN
RYFQLTYDTE LANCILGTAI RLAVDMELNR KSSYKSLDFE EAIRRRRMWW HCFCTDKLYS
LMLSRPPIVG ERDMDMLTDQ NYYEVIKTNI LPDLIDKKED LDKITDVNSA LNVVVNFCQH
ISLFISYYVS KLVSIESKIY STCFAVRSTL DLSFDAMLDK IKDLNDSLNN WRDNLHVSMK
LKSYKQYLSV LYAQKSQENP ALSFEIACSR VLNCHFRALY SKVILSMMTT SLLIDNERLY
KGSRHDIPQL FILFSSQYLN ASKEMLQLFQ GINYQAHMYN EVMYQFSTAM FVLFFYVVDN
MNDLKKKGEV KEIIDILKKS YDRLVGENDE QLLFDNVKWN TLIVFYSHFL KYVLQRYHAL
NDSTSIFDSK PYDETITKVI MHSRKIKDET VDQLIMSLKS YGSLHSLQKG NEADLADDGL
NTNDISSEDF AEEAPINLFG ELSVEILKLL KSHSPISNFG DLSPSSNRKG ISDDSSLYPI
RSDLTSLVYP IHSSDTGDTL SSGLETPENS NFNSDSGIKE DFEAFRALLP LGKLIYDRDY
SFVNTFRDYE
