GR64F_DROME
ID GR64F_DROME Reviewed; 469 AA.
AC P83297; Q9VZJ5;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Gustatory receptor for sugar taste 64f;
GN Name=Gr64f; ORFNames=CG32255;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP IDENTIFICATION.
RX PubMed=11516643; DOI=10.1016/s0960-9822(01)00258-5;
RA Dunipace L., Meister S., McNealy C., Amrein H.;
RT "Spatially restricted expression of candidate taste receptors in the
RT Drosophila gustatory system.";
RL Curr. Biol. 11:822-835(2001).
RN [4]
RP PREDICTION OF FUNCTION.
RX PubMed=14608037; DOI=10.1073/pnas.2335847100;
RA Robertson H.M., Warr C.G., Carlson J.R.;
RT "Molecular evolution of the insect chemoreceptor gene superfamily in
RT Drosophila melanogaster.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:14537-14542(2003).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=17715294; DOI=10.1073/pnas.0702421104;
RA Jiao Y., Moon S.J., Montell C.;
RT "A Drosophila gustatory receptor required for the responses to sucrose,
RT glucose, and maltose identified by mRNA tagging.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:14110-14115(2007).
RN [6]
RP FUNCTION.
RX PubMed=19026541; DOI=10.1016/j.cub.2008.10.009;
RA Jiao Y., Moon S.J., Wang X., Ren Q., Montell C.;
RT "Gr64f is required in combination with other gustatory receptors for sugar
RT detection in Drosophila.";
RL Curr. Biol. 18:1797-1801(2008).
RN [7]
RP FUNCTION.
RX PubMed=23850280; DOI=10.1016/j.cub.2013.06.028;
RA Mishra D., Miyamoto T., Rezenom Y.H., Broussard A., Yavuz A., Slone J.,
RA Russell D.H., Amrein H.;
RT "The molecular basis of sugar sensing in Drosophila larvae.";
RL Curr. Biol. 23:1466-1471(2013).
RN [8]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=23418550; DOI=10.1371/journal.pone.0056304;
RA Miyamoto T., Chen Y., Slone J., Amrein H.;
RT "Identification of a Drosophila glucose receptor using Ca2+ imaging of
RT single chemosensory neurons.";
RL PLoS ONE 8:E56304-E56304(2013).
CC -!- FUNCTION: One of the few identified sugar gustatory receptors
CC identified so far and which promotes the starvation-induced increase of
CC feeding motivation. Required in combination with Gr64a to detect
CC sucrose, maltose, and glucose. {ECO:0000269|PubMed:17715294,
CC ECO:0000269|PubMed:19026541, ECO:0000269|PubMed:23418550,
CC ECO:0000269|PubMed:23850280}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in Gr5a-expressing sugar-sensing cells.
CC {ECO:0000269|PubMed:17715294, ECO:0000269|PubMed:23418550}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily. Gustatory
CC receptor (GR) family. Gr5a subfamily. {ECO:0000305}.
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DR EMBL; AE014296; AAF47826.3; -; Genomic_DNA.
DR RefSeq; NP_728924.2; NM_168052.3.
DR AlphaFoldDB; P83297; -.
DR SMR; P83297; -.
DR STRING; 7227.FBpp0073057; -.
DR GlyGen; P83297; 1 site.
DR PaxDb; P83297; -.
DR PRIDE; P83297; -.
DR EnsemblMetazoa; FBtr0073201; FBpp0073057; FBgn0052255.
DR GeneID; 117477; -.
DR KEGG; dme:Dmel_CG32255; -.
DR CTD; 117477; -.
DR FlyBase; FBgn0052255; Gr64f.
DR VEuPathDB; VectorBase:FBgn0052255; -.
DR eggNOG; ENOG502QTKP; Eukaryota.
DR HOGENOM; CLU_043581_0_0_1; -.
DR InParanoid; P83297; -.
DR OMA; FHEDQDL; -.
DR OrthoDB; 622663at2759; -.
DR PhylomeDB; P83297; -.
DR BioGRID-ORCS; 117477; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 117477; -.
DR PRO; PR:P83297; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0052255; Expressed in testis and 1 other tissue.
DR ExpressionAtlas; P83297; baseline and differential.
DR Genevisible; P83297; DM.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0033041; F:sweet taste receptor activity; IMP:FlyBase.
DR GO; GO:0008527; F:taste receptor activity; NAS:UniProtKB.
DR GO; GO:0050912; P:detection of chemical stimulus involved in sensory perception of taste; IMP:FlyBase.
DR GO; GO:0007606; P:sensory perception of chemical stimulus; IBA:GO_Central.
DR GO; GO:0050916; P:sensory perception of sweet taste; IMP:FlyBase.
DR GO; GO:0050909; P:sensory perception of taste; NAS:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR009318; Gustatory_rcpt.
DR PANTHER; PTHR21421; PTHR21421; 1.
DR Pfam; PF06151; Trehalose_recp; 1.
DR PIRSF; PIRSF038981; GRP; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Membrane; Receptor; Reference proteome;
KW Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..469
FT /note="Gustatory receptor for sugar taste 64f"
FT /id="PRO_0000216533"
FT TOPO_DOM 1..117
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 118..138
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 139..146
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 147..167
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 168..199
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 200..220
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 221..265
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 266..286
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 287..330
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 331..351
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 352..353
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 354..374
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 375..435
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 436..456
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 457..469
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT CARBOHYD 230
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 469 AA; 54576 MW; E47B0232DF424AAA CRC64;
MKILPKLERK LRRLKKRVTR TSLFRKLDLV HESARKKAFQ ESCETYKNQI ENEYEIRNSL
PKLSRSDKEA FLSDGSFHQA VGRVLLVAEF FAMMPVKGVT GKHPSDLSFS WRNIRTCFSL
LFIASSLANF GLSLFKVLNN PISFNSIKPI IFRGSVLLVL IVALNLARQW PQLMMYWHTV
EKDLPQYKTQ LTKWKMGHTI SMVMLLGMML SFAEHILSMV SAINYASFCN RTADPIQNYF
LRTNDEIFFV TSYSTTLALW GKFQNVFSTF IWNYMDLFVM IVSIGLASKF RQLNDDLRNF
KGMNMAPSYW SERRIQYRNI CILCDKMDDA ISLITMVSFS NNLYFICVQL LRSLNTMPSV
AHAVYFYFSL IFLIGRTLAV SLYSSSVHDE SRLTLRYLRC VPKESWCPEV KRFTEEVISD
EVALTGMKFF HLTRKLVLSV AGTIVTYELV LIQFHEDNDL WDCDQSYYS
