ID   GNKL2_PICSI             Reviewed;         131 AA.
AC   D5A8I3;
DT   18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT   15-JUN-2010, sequence version 1.
DT   03-AUG-2022, entry version 18.
DE   RecName: Full=Antifungal protein ginkbilobin-like protein 2 {ECO:0000305};
DE   Flags: Precursor;
OS   Picea sitchensis (Sitka spruce) (Pinus sitchensis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Picea.
OX   NCBI_TaxID=3332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Reid K.E., Liao N., Chan S., Docking R., Taylor G., Moore R., Mayo M.,
RA   Munro S., King J., Yanchuk A., Holt R., Jones S., Marra M., Ritland C.E.,
RA   Ritland K., Bohlmann J.;
RL   Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   IDENTIFICATION.
RX   PubMed=26315821; DOI=10.1007/s00709-015-0876-4;
RA   Gao N., Wadhwani P., Muehlhaeuser P., Liu Q., Riemann M., Ulrich A.S.,
RA   Nick P.;
RT   "An antifungal protein from Ginkgo biloba binds actin and can trigger cell
RT   death.";
RL   Protoplasma 253:1159-1174(2016).
CC   -!- FUNCTION: Exerts antifungal activity through its carbohydrate-binding
CC       specificity. {ECO:0000250|UniProtKB:A4ZDL6}.
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DR   EMBL; BT122477; ADE75852.1; -; mRNA.
DR   AlphaFoldDB; D5A8I3; -.
DR   SMR; D5A8I3; -.
DR   GO; GO:0005537; F:mannose binding; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.430.20; -; 1.
DR   InterPro; IPR002902; GNK2.
DR   InterPro; IPR038408; GNK2_sf.
DR   Pfam; PF01657; Stress-antifung; 1.
DR   PROSITE; PS51473; GNK2; 1.
PE   2: Evidence at transcript level;
KW   Antibiotic; Antimicrobial; Disulfide bond; Fungicide; Lectin;
KW   Mannose-binding; Plant defense; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..131
FT                   /note="Antifungal protein ginkbilobin-like protein 2"
FT                   /id="PRO_5003069107"
FT   DOMAIN          26..131
FT                   /note="Gnk2-homologous"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT   BINDING         34
FT                   /ligand="alpha-D-mannopyranose"
FT                   /ligand_id="ChEBI:CHEBI:28729"
FT                   /evidence="ECO:0000250|UniProtKB:A4ZDL6"
FT   BINDING         116
FT                   /ligand="alpha-D-mannopyranose"
FT                   /ligand_id="ChEBI:CHEBI:28729"
FT                   /evidence="ECO:0000250|UniProtKB:A4ZDL6"
FT   BINDING         127
FT                   /ligand="alpha-D-mannopyranose"
FT                   /ligand_id="ChEBI:CHEBI:28729"
FT                   /evidence="ECO:0000250|UniProtKB:A4ZDL6"
FT   DISULFID        33..109
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT   DISULFID        85..94
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT   DISULFID        97..122
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
SQ   SEQUENCE   131 AA;  13908 MW;  076705DA6CE699B6 CRC64;
     MSPSSVALLA AVVIVVMGGA AEAANTKFVS ASCNTEKIAR GSPFFNNLRD AIEDLRQNTA
     YIGYDYKTSH VGSGGAPTAY GRAICKQSIS QSNCTSCLSN VATRIYGICG NAIGARVQLT
     DCYMQYEQRS F