GNKL2_PICGL
ID GNKL2_PICGL Reviewed; 133 AA.
AC Q40858;
DT 18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 52.
DE RecName: Full=Antifungal protein ginkbilobin-like protein 2 {ECO:0000305};
DE AltName: Full=Embryo-abundant protein 35 {ECO:0000305};
DE Flags: Precursor;
GN Name=EMB35 {ECO:0000312|EMBL:AAB01601.1};
OS Picea glauca (White spruce) (Pinus glauca).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Picea.
OX NCBI_TaxID=3330;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, AND INDUCTION BY ABA.
RC TISSUE=Embryo;
RX PubMed=8771802; DOI=10.1007/bf00195740;
RA Dong J.Z., Dunstan D.I.;
RT "Expression of abundant mRNAs during somatic embryogenesis of white spruce
RT [Picea glauca (Moench) Voss].";
RL Planta 199:459-466(1996).
RN [2]
RP IDENTIFICATION.
RX PubMed=26315821; DOI=10.1007/s00709-015-0876-4;
RA Gao N., Wadhwani P., Muehlhaeuser P., Liu Q., Riemann M., Ulrich A.S.,
RA Nick P.;
RT "An antifungal protein from Ginkgo biloba binds actin and can trigger cell
RT death.";
RL Protoplasma 253:1159-1174(2016).
CC -!- FUNCTION: Exerts antifungal activity through its carbohydrate-binding
CC specificity. {ECO:0000250|UniProtKB:A4ZDL6}.
CC -!- DEVELOPMENTAL STAGE: Expressed during embryo development.
CC {ECO:0000269|PubMed:8771802}.
CC -!- INDUCTION: Induced by abscisic acid (ABA).
CC {ECO:0000269|PubMed:8771802}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L47605; AAB01601.1; -; mRNA.
DR PIR; T09282; T09282.
DR AlphaFoldDB; Q40858; -.
DR SMR; Q40858; -.
DR GO; GO:0005537; F:mannose binding; IEA:UniProtKB-KW.
DR GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR GO; GO:0050832; P:defense response to fungus; ISS:UniProtKB.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR GO; GO:0009737; P:response to abscisic acid; IEP:UniProtKB.
DR Gene3D; 3.30.430.20; -; 1.
DR InterPro; IPR002902; GNK2.
DR InterPro; IPR038408; GNK2_sf.
DR Pfam; PF01657; Stress-antifung; 1.
DR PROSITE; PS51473; GNK2; 1.
PE 2: Evidence at transcript level;
KW Antibiotic; Antimicrobial; Disulfide bond; Fungicide; Lectin;
KW Mannose-binding; Plant defense; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..133
FT /note="Antifungal protein ginkbilobin-like protein 2"
FT /id="PRO_5004231631"
FT DOMAIN 28..133
FT /note="Gnk2-homologous"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT BINDING 36
FT /ligand="alpha-D-mannopyranose"
FT /ligand_id="ChEBI:CHEBI:28729"
FT /evidence="ECO:0000250|UniProtKB:A4ZDL6"
FT BINDING 118
FT /ligand="alpha-D-mannopyranose"
FT /ligand_id="ChEBI:CHEBI:28729"
FT /evidence="ECO:0000250|UniProtKB:A4ZDL6"
FT BINDING 129
FT /ligand="alpha-D-mannopyranose"
FT /ligand_id="ChEBI:CHEBI:28729"
FT /evidence="ECO:0000250|UniProtKB:A4ZDL6"
FT DISULFID 87..96
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT DISULFID 99..124
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
SQ SEQUENCE 133 AA; 13756 MW; 1B3F072CDB875B59 CRC64;
MSMGSFGFAL AVMVLAVLVA SAAGAPNTNL VSSACNGNKI PSGNPFFNNL GALLVDLEKN
TAFSGFDYKA SRAGSGGAPT AYGRGVCKQS ISQSDCTACL TNLGGRIWGI SKNAIGARVQ
LTDCFIRYEQ YSI
