ID   GNKL1_PICSI             Reviewed;         133 AA.
AC   A9NQT1;
DT   18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 1.
DT   03-AUG-2022, entry version 29.
DE   RecName: Full=Antifungal protein ginkbilobin-like protein 1 {ECO:0000305};
DE   Flags: Precursor;
OS   Picea sitchensis (Sitka spruce) (Pinus sitchensis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Picea.
OX   NCBI_TaxID=3332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Bark {ECO:0000312|EMBL:ABK22992.1};
RX   PubMed=18854048; DOI=10.1186/1471-2164-9-484;
RA   Ralph S.G., Chun H.J.E., Kolosova N., Cooper D., Oddy C., Ritland C.E.,
RA   Kirkpatrick R., Moore R., Barber S., Holt R.A., Jones S.J.M., Marra M.A.,
RA   Douglas C.J., Ritland K., Bohlmann J.;
RT   "A conifer genomics resource of 200,000 spruce (Picea spp.) ESTs and 6,464
RT   high-quality, sequence-finished full-length cDNAs for Sitka spruce (Picea
RT   sitchensis).";
RL   BMC Genomics 9:484-484(2008).
RN   [2]
RP   IDENTIFICATION.
RX   PubMed=26315821; DOI=10.1007/s00709-015-0876-4;
RA   Gao N., Wadhwani P., Muehlhaeuser P., Liu Q., Riemann M., Ulrich A.S.,
RA   Nick P.;
RT   "An antifungal protein from Ginkgo biloba binds actin and can trigger cell
RT   death.";
RL   Protoplasma 253:1159-1174(2016).
CC   -!- FUNCTION: Exerts antifungal activity through its carbohydrate-binding
CC       specificity. {ECO:0000250|UniProtKB:A4ZDL6}.
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DR   EMBL; EF083656; ABK22992.1; -; mRNA.
DR   AlphaFoldDB; A9NQT1; -.
DR   SMR; A9NQT1; -.
DR   GO; GO:0005537; F:mannose binding; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; ISS:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.430.20; -; 1.
DR   InterPro; IPR002902; GNK2.
DR   InterPro; IPR038408; GNK2_sf.
DR   Pfam; PF01657; Stress-antifung; 1.
DR   PROSITE; PS51473; GNK2; 1.
PE   2: Evidence at transcript level;
KW   Antibiotic; Antimicrobial; Disulfide bond; Fungicide; Lectin;
KW   Mannose-binding; Plant defense; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..133
FT                   /note="Antifungal protein ginkbilobin-like protein 1"
FT                   /id="PRO_5002741214"
FT   DOMAIN          28..133
FT                   /note="Gnk2-homologous"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT   BINDING         36
FT                   /ligand="alpha-D-mannopyranose"
FT                   /ligand_id="ChEBI:CHEBI:28729"
FT                   /evidence="ECO:0000250|UniProtKB:A4ZDL6"
FT   BINDING         118
FT                   /ligand="alpha-D-mannopyranose"
FT                   /ligand_id="ChEBI:CHEBI:28729"
FT                   /evidence="ECO:0000250|UniProtKB:A4ZDL6"
FT   BINDING         129
FT                   /ligand="alpha-D-mannopyranose"
FT                   /ligand_id="ChEBI:CHEBI:28729"
FT                   /evidence="ECO:0000250|UniProtKB:A4ZDL6"
FT   DISULFID        35..111
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT   DISULFID        87..96
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT   DISULFID        99..124
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
SQ   SEQUENCE   133 AA;  13879 MW;  BDF6EDC020F75815 CRC64;
     MSMGSFGFAL AVMVLAVLVA SAAGAPNTNF VSSACNTKKI PSGNPFFNNL GALLVNLEKN
     TAFSGYDYKA SRAGSGGAPT AYGRGVCKQS ISQSDCTACL TNLGGRIWGI CKNAIGARVQ
     LTDCFIRYEQ YSI