ID   GNKL1_PICGL             Reviewed;         141 AA.
AC   Q40849; Q3I3X1; Q3I3Y8;
DT   18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 53.
DE   RecName: Full=Antifungal protein ginkbilobin-like protein 1 {ECO:0000305};
DE   AltName: Full=Embryo-abundant protein 24 {ECO:0000305};
DE   Flags: Precursor;
GN   Name=EMB24 {ECO:0000312|EMBL:AAB01559.1, ECO:0000312|EMBL:ABA54794.1,
GN   ECO:0000312|EMBL:ABA54811.1};
OS   Picea glauca (White spruce) (Pinus glauca).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Picea.
OX   NCBI_TaxID=3330;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC   TISSUE=Embryo;
RX   PubMed=10350098; DOI=10.1023/a:1006146622614;
RA   Dong J.-Z., Dunstan D.I.;
RT   "Cloning and characterization of six embryogenesis-associated cDNAs from
RT   somatic embryos of Picea glauca and their comparative expression during
RT   zygotic embryogenesis.";
RL   Plant Mol. Biol. 39:859-864(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 33-141.
RC   TISSUE=Needle {ECO:0000312|EMBL:ABA54811.1};
RX   AGRICOLA=IND43783961;
RA   Lamothe M., Meirmans P., Isabel N.;
RT   "A set of polymorphic EST-derived markers for Picea species.";
RL   Mol. Ecol. Notes 6:237-240(2006).
RN   [3]
RP   IDENTIFICATION.
RX   PubMed=26315821; DOI=10.1007/s00709-015-0876-4;
RA   Gao N., Wadhwani P., Muehlhaeuser P., Liu Q., Riemann M., Ulrich A.S.,
RA   Nick P.;
RT   "An antifungal protein from Ginkgo biloba binds actin and can trigger cell
RT   death.";
RL   Protoplasma 253:1159-1174(2016).
CC   -!- FUNCTION: Exerts antifungal activity through its carbohydrate-binding
CC       specificity. {ECO:0000250|UniProtKB:A4ZDL6}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during somatic and zygotic embryo
CC       development. {ECO:0000269|PubMed:10350098}.
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DR   EMBL; L47671; AAB01559.1; -; mRNA.
DR   EMBL; DQ120076; ABA54794.1; -; Genomic_DNA.
DR   EMBL; DQ120093; ABA54811.1; -; Genomic_DNA.
DR   PIR; T09251; T09251.
DR   AlphaFoldDB; Q40849; -.
DR   SMR; Q40849; -.
DR   GO; GO:0005537; F:mannose binding; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; ISS:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.430.20; -; 1.
DR   InterPro; IPR002902; GNK2.
DR   InterPro; IPR038408; GNK2_sf.
DR   Pfam; PF01657; Stress-antifung; 1.
DR   PROSITE; PS51473; GNK2; 1.
PE   2: Evidence at transcript level;
KW   Antibiotic; Antimicrobial; Disulfide bond; Fungicide; Lectin;
KW   Mannose-binding; Plant defense; Signal.
FT   SIGNAL          1..32
FT                   /evidence="ECO:0000255"
FT   CHAIN           33..141
FT                   /note="Antifungal protein ginkbilobin-like protein 1"
FT                   /id="PRO_5004231582"
FT   DOMAIN          36..141
FT                   /note="Gnk2-homologous"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT   BINDING         44
FT                   /ligand="alpha-D-mannopyranose"
FT                   /ligand_id="ChEBI:CHEBI:28729"
FT                   /evidence="ECO:0000250|UniProtKB:A4ZDL6"
FT   BINDING         126
FT                   /ligand="alpha-D-mannopyranose"
FT                   /ligand_id="ChEBI:CHEBI:28729"
FT                   /evidence="ECO:0000250|UniProtKB:A4ZDL6"
FT   BINDING         137
FT                   /ligand="alpha-D-mannopyranose"
FT                   /ligand_id="ChEBI:CHEBI:28729"
FT                   /evidence="ECO:0000250|UniProtKB:A4ZDL6"
FT   DISULFID        43..119
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT   DISULFID        95..104
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT   DISULFID        107..132
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT   CONFLICT        56
FT                   /note="T -> N (in Ref. 2; ABA54794)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        61..63
FT                   /note="MLA -> VLT (in Ref. 2; ABA54794)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   141 AA;  14937 MW;  9C84DBE39A0783AD CRC64;
     MSISSKFQLR SSTSLLLLVA LMVVMGMDGA AAAPNTNFVS SACNTQKIPS GNPFFTNLRA
     MLADLKQNTA FSGFDYKTSR AGSGGAPTAY GRAICKSSIS QSDCSACLSN LVGRIWGICS
     NAIGARVQLT DCFIQYEQHS F