ID   GNK1_GINBI              Reviewed;          40 AA.
AC   P83171;
DT   25-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 42.
DE   RecName: Full=Antifungal protein ginkbilobin-1 {ECO:0000305};
DE   AltName: Full=Ginkbilobin {ECO:0000303|PubMed:11118300};
DE            Short=GNL {ECO:0000303|PubMed:11118300};
DE   Flags: Fragment;
GN   Name=GNK1 {ECO:0000305}; Synonyms=GNL {ECO:0000303|PubMed:11118300};
OS   Ginkgo biloba (Ginkgo) (Maidenhair tree).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Ginkgoidae; Ginkgoales; Ginkgoaceae; Ginkgo.
OX   NCBI_TaxID=3311;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, AND TISSUE SPECIFICITY.
RC   TISSUE=Seed;
RX   PubMed=11118300; DOI=10.1006/bbrc.2000.3929;
RA   Wang H., Ng T.B.;
RT   "Ginkbilobin, a novel antifungal protein from Ginkgo biloba seeds with
RT   sequence similarity to embryo-abundant protein.";
RL   Biochem. Biophys. Res. Commun. 279:407-411(2000).
CC   -!- FUNCTION: Possesses antifungal activity against B.cinerea,
CC       M.arachidicola, F.oxysporum, R.solani and C.comatus and moderate
CC       antibacterial activity against S.aureus, P.aeruginosa and E.coli.
CC       Inhibits HIV-1 reverse transcriptase and proliferation of murine
CC       splenocytes (PubMed:11118300). Exerts antifungal activity through its
CC       carbohydrate-binding specificity (By similarity).
CC       {ECO:0000250|UniProtKB:A4ZDL6, ECO:0000269|PubMed:11118300}.
CC   -!- TISSUE SPECIFICITY: Expressed in seeds (at the protein level).
CC       {ECO:0000269|PubMed:11118300}.
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DR   AlphaFoldDB; P83171; -.
DR   SMR; P83171; -.
DR   GO; GO:0005537; F:mannose binding; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IDA:UniProtKB.
DR   GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   GO; GO:0008285; P:negative regulation of cell population proliferation; TAS:UniProtKB.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Fungicide; Lectin;
KW   Mannose-binding; Plant defense.
FT   CHAIN           1..>40
FT                   /note="Antifungal protein ginkbilobin-1"
FT                   /id="PRO_0000087499"
FT   DOMAIN          3..40
FT                   /note="Gnk2-homologous"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00806"
FT   BINDING         11
FT                   /ligand="alpha-D-mannopyranose"
FT                   /ligand_id="ChEBI:CHEBI:28729"
FT                   /evidence="ECO:0000250|UniProtKB:A4ZDL6"
FT   NON_TER         40
FT                   /evidence="ECO:0000303|PubMed:11118300"
SQ   SEQUENCE   40 AA;  4214 MW;  7DE065FB52877366 CRC64;
     ANTAFVSSAH NTQKIPAGAP FNRNLRAMLA DLRQNAAFAG