ID   GNGF_MYCTU              Reviewed;         342 AA.
AC   P9WMU5; L0T6L6; P71691;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Putative gluconeogenesis factor {ECO:0000255|HAMAP-Rule:MF_00973};
GN   OrderedLocusNames=Rv1422; ORFNames=MTCY21B4.40;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   PHOSPHORYLATION AT THR-325, AND MUTAGENESIS OF THR-325.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=15985609; DOI=10.1101/gad.1311105;
RA   Kang C.M., Abbott D.W., Park S.T., Dascher C.C., Cantley L.C., Husson R.N.;
RT   "The Mycobacterium tuberculosis serine/threonine kinases PknA and PknB:
RT   substrate identification and regulation of cell shape.";
RL   Genes Dev. 19:1692-1704(2005).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Required for morphogenesis under gluconeogenic growth
CC       conditions. {ECO:0000255|HAMAP-Rule:MF_00973}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00973}.
CC   -!- PTM: Phosphorylated by PknA and/or PknB. {ECO:0000269|PubMed:15985609}.
CC   -!- SIMILARITY: Belongs to the gluconeogenesis factor family.
CC       {ECO:0000255|HAMAP-Rule:MF_00973}.
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DR   EMBL; AL123456; CCP44181.1; -; Genomic_DNA.
DR   PIR; C70903; C70903.
DR   RefSeq; NP_215938.1; NC_000962.3.
DR   RefSeq; WP_003407352.1; NZ_NVQJ01000038.1.
DR   AlphaFoldDB; P9WMU5; -.
DR   SMR; P9WMU5; -.
DR   STRING; 83332.Rv1422; -.
DR   iPTMnet; P9WMU5; -.
DR   PaxDb; P9WMU5; -.
DR   DNASU; 886670; -.
DR   GeneID; 45425400; -.
DR   GeneID; 886670; -.
DR   KEGG; mtu:Rv1422; -.
DR   TubercuList; Rv1422; -.
DR   eggNOG; COG0391; Bacteria.
DR   OMA; LCGDDDW; -.
DR   PhylomeDB; P9WMU5; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0043743; F:LPPG:FO 2-phospho-L-lactate transferase activity; IEA:InterPro.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10680; -; 1.
DR   HAMAP; MF_00973; Gluconeogen_factor; 1.
DR   InterPro; IPR002882; CofD.
DR   InterPro; IPR038136; CofD-like_dom_sf.
DR   InterPro; IPR010119; Gluconeogen_factor.
DR   PANTHER; PTHR30135; PTHR30135; 1.
DR   Pfam; PF01933; CofD; 1.
DR   SUPFAM; SSF142338; SSF142338; 1.
DR   TIGRFAMs; TIGR01826; CofD_related; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Phosphoprotein; Reference proteome.
FT   CHAIN           1..342
FT                   /note="Putative gluconeogenesis factor"
FT                   /id="PRO_0000107811"
FT   REGION          318..342
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         325
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000269|PubMed:15985609"
FT   MUTAGEN         325
FT                   /note="T->A,S: Lack of phosphorylation."
FT                   /evidence="ECO:0000269|PubMed:15985609"
SQ   SEQUENCE   342 AA;  35823 MW;  D38C002F8C0BA16D CRC64;
     MTDGIVALGG GHGLYATLSA ARRLTPYVTA VVTVADDGGS SGRLRSELDV VPPGDLRMAL
     AALASDSPHG RLWATILQHR FGGSGALAGH PIGNLMLAGL SEVLADPVAA LDELGRILGV
     KGRVLPMCPV ALQIEADVSG LEADPRMFRL IRGQVAIATT PGKVRRVRLL PTDPPATRQA
     VDAIMAADLV VLGPGSWFTS VIPHVLVPGL AAALRATSAR RALVLNLVAE PGETAGFSVE
     RHLHVLAQHA PGFTVHDIII DAERVPSERE REQLRRTATM LQAEVHFADV ARPGTPLHDP
     GKLAAVLDGV CARDVGASEP PVAATQEIPI DGGRPRGDDA WR