ID   GNGF_BACSU              Reviewed;         317 AA.
AC   O06974;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Gluconeogenesis factor {ECO:0000255|HAMAP-Rule:MF_00973};
GN   Name=mgfK; Synonyms=yvcK; OrderedLocusNames=BSU34760;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RA   Denizot F.;
RL   Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=168;
RX   PubMed=16272399; DOI=10.1099/mic.0.28172-0;
RA   Gorke B., Foulquier E., Galinier A.;
RT   "YvcK of Bacillus subtilis is required for a normal cell shape and for
RT   growth on Krebs cycle intermediates and substrates of the pentose phosphate
RT   pathway.";
RL   Microbiology 151:3777-3791(2005).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, AND OVERPRODUCTION.
RX   PubMed=21320184; DOI=10.1111/j.1365-2958.2011.07587.x;
RA   Foulquier E., Pompeo F., Bernadac A., Espinosa L., Galinier A.;
RT   "The YvcK protein is required for morphogenesis via localization of PBP1
RT   under gluconeogenic growth conditions in Bacillus subtilis.";
RL   Mol. Microbiol. 80:309-318(2011).
CC   -!- FUNCTION: Required for morphogenesis under gluconeogenic growth
CC       conditions. Required, in gluconeogenic growth conditions, for the
CC       correct localization of PBP1 and hence for displaying a normal rod
CC       shape. {ECO:0000255|HAMAP-Rule:MF_00973, ECO:0000269|PubMed:16272399,
CC       ECO:0000269|PubMed:21320184}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00973,
CC       ECO:0000269|PubMed:21320184}. Note=Organized into coiled helical
CC       structures extending from one pole to the other. Localization does not
CC       require MreB.
CC   -!- DISRUPTION PHENOTYPE: Deletion leads to growth defects when cells are
CC       grown on minimal medium containing ribose, gluconate, citrate, fumarate
CC       or succinate, but does not affect growth and morphology on minimal
CC       medium containing glycolytic substrates such as glucose, sucrose or
CC       glycerol. Mutants exhibit media-dependent filamentous or L-shape-like
CC       aberrant morphologies. {ECO:0000269|PubMed:16272399}.
CC   -!- MISCELLANEOUS: When bacteria are grown on a gluconeogenic carbon
CC       source, overproduction can restore a normal rod-shaped morphology in a
CC       mreB mutant by rescuing the PBP1 localization.
CC       {ECO:0000305|PubMed:21320184}.
CC   -!- SIMILARITY: Belongs to the gluconeogenesis factor family.
CC       {ECO:0000255|HAMAP-Rule:MF_00973}.
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DR   EMBL; Z94043; CAB08058.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15481.2; -; Genomic_DNA.
DR   PIR; A70032; A70032.
DR   RefSeq; NP_391356.2; NC_000964.3.
DR   RefSeq; WP_003244450.1; NZ_JNCM01000033.1.
DR   AlphaFoldDB; O06974; -.
DR   SMR; O06974; -.
DR   STRING; 224308.BSU34760; -.
DR   PaxDb; O06974; -.
DR   PRIDE; O06974; -.
DR   EnsemblBacteria; CAB15481; CAB15481; BSU_34760.
DR   GeneID; 936527; -.
DR   KEGG; bsu:BSU34760; -.
DR   PATRIC; fig|224308.179.peg.3764; -.
DR   eggNOG; COG0391; Bacteria.
DR   InParanoid; O06974; -.
DR   OMA; LCGDDDW; -.
DR   PhylomeDB; O06974; -.
DR   BioCyc; BSUB:BSU34760-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0043743; F:LPPG:FO 2-phospho-L-lactate transferase activity; IEA:InterPro.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.10680; -; 1.
DR   HAMAP; MF_00973; Gluconeogen_factor; 1.
DR   InterPro; IPR002882; CofD.
DR   InterPro; IPR038136; CofD-like_dom_sf.
DR   InterPro; IPR010119; Gluconeogen_factor.
DR   PANTHER; PTHR30135; PTHR30135; 1.
DR   Pfam; PF01933; CofD; 1.
DR   SUPFAM; SSF142338; SSF142338; 1.
DR   TIGRFAMs; TIGR01826; CofD_related; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome.
FT   CHAIN           1..317
FT                   /note="Gluconeogenesis factor"
FT                   /id="PRO_0000107804"
SQ   SEQUENCE   317 AA;  34687 MW;  6086D29117392CCD CRC64;
     MGQKPKIAIF GGGTGLSVLL RGLKHKPVDI TAIVTVADDG GSSGRLRNEL KIPPPGDIRN
     VLAALSDVEP LVEDLFQHRF NKGNDLTGHS LGNLILAAMT NITGDFFHAV TEMSKVLNVR
     GKVLPAANAS VVLHAEMEDG RVVSGESTIP EYGQRIKRVF LTPEQIDPLP ETIDVIREAD
     LIIIGPGSLY TSILPNLLVP KIGEEVIKAP AKKVYICNVM TQPGETLHYT AADHVKALNQ
     HMGCGFIDTI LVNSEDIPDE IKRKYEMESA RPVDFDIEEL KAMGLEVIRD QIVTYKNDVI
     RHDTHKVASL LVDLLKE