ID   AMGDS_STRR6             Reviewed;         441 AA.
AC   Q8CWR6;
DT   19-FEB-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Alpha-monoglucosyldiacylglycerol synthase;
DE            Short=Alpha-MGS;
DE            Short=MGlcDAG synthase;
DE            EC=2.4.1.337 {ECO:0000269|PubMed:11294844};
DE   AltName: Full=1,2-Diacylglycerol 3-glucosyltransferase;
DE   AltName: Full=UDP-glucose:1,2-diacylglycerol 3-alpha-D-glucosyltransferase;
GN   OrderedLocusNames=spr0982;
OS   Streptococcus pneumoniae (strain ATCC BAA-255 / R6).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=171101;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-255 / R6;
RX   PubMed=11544234; DOI=10.1128/jb.183.19.5709-5717.2001;
RA   Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S.,
RA   DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C.,
RA   Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J.,
RA   Lee L.N., Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M.,
RA   McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B.,
RA   Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y.,
RA   Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R.,
RA   Rosteck P.R. Jr., Skatrud P.L., Glass J.I.;
RT   "Genome of the bacterium Streptococcus pneumoniae strain R6.";
RL   J. Bacteriol. 183:5709-5717(2001).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, AND ACTIVITY REGULATION.
RC   STRAIN=CCUG 3030;
RX   PubMed=11294844; DOI=10.1074/jbc.m102576200;
RA   Berg S., Edman M., Li L., Wikstrom M., Wieslander A.;
RT   "Sequence properties of the 1,2-diacylglycerol 3-glucosyltransferase from
RT   Acholeplasma laidlawii membranes. Recognition of a large group of lipid
RT   glycosyltransferases in eubacteria and archaea.";
RL   J. Biol. Chem. 276:22056-22063(2001).
CC   -!- FUNCTION: Glucosyltransferase involved in the biosynthesis of the non-
CC       bilayer-prone membrane lipid alpha-monoglucosyldiacylglycerol. This is
CC       a major component for maintaining a certain anionic lipid surface
CC       charge density, for balancing the bilayer to non-bilayer phase
CC       equilibria and for keeping a constant lipid bilayer spontaneous
CC       curvature (curvature packing stress). Catalyzes the transfer of a
CC       glucosyl residue from UDP-Glc to diacylglycerol (DAG) acceptor to form
CC       the corresponding alpha-glucosyl-DAG (1,2-diacyl-3-O-(alpha-D-
CC       glucopyranosyl)-sn-glycerol). It can only use UDP-Glc as sugar donor.
CC       {ECO:0000269|PubMed:11294844}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + UDP-alpha-D-glucose = a 1,2-diacyl-
CC         3-O-(alpha-D-glucopyranosyl)-sn-glycerol + H(+) + UDP;
CC         Xref=Rhea:RHEA:47612, ChEBI:CHEBI:15378, ChEBI:CHEBI:17670,
CC         ChEBI:CHEBI:17815, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885;
CC         EC=2.4.1.337; Evidence={ECO:0000269|PubMed:11294844};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- ACTIVITY REGULATION: Activated by the negatively charged lipid
CC       phosphatidylglycerol (PG). {ECO:0000269|PubMed:11294844}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC       Glycosyltransferase 4 subfamily. {ECO:0000305}.
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DR   EMBL; AE007317; AAK99786.1; -; Genomic_DNA.
DR   PIR; F97994; F97994.
DR   RefSeq; NP_358576.1; NC_003098.1.
DR   RefSeq; WP_001219432.1; NC_003098.1.
DR   AlphaFoldDB; Q8CWR6; -.
DR   SMR; Q8CWR6; -.
DR   STRING; 171101.spr0982; -.
DR   CAZy; GT4; Glycosyltransferase Family 4.
DR   EnsemblBacteria; AAK99786; AAK99786; spr0982.
DR   GeneID; 60233307; -.
DR   KEGG; spr:spr0982; -.
DR   PATRIC; fig|171101.6.peg.1068; -.
DR   eggNOG; COG0438; Bacteria.
DR   HOGENOM; CLU_009583_2_0_9; -.
DR   OMA; FFCPNAG; -.
DR   BioCyc; MetaCyc:MON-20028; -.
DR   Proteomes; UP000000586; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016757; F:glycosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0016758; F:hexosyltransferase activity; IDA:UniProtKB.
DR   GO; GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0046467; P:membrane lipid biosynthetic process; IDA:UniProtKB.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   InterPro; IPR028098; Glyco_trans_4-like_N.
DR   Pfam; PF13439; Glyco_transf_4; 1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Cell membrane; Glycerol metabolism;
KW   Glycosyltransferase; Lipid biosynthesis; Lipid metabolism; Magnesium;
KW   Membrane; Reference proteome; Transferase.
FT   CHAIN           1..441
FT                   /note="Alpha-monoglucosyldiacylglycerol synthase"
FT                   /id="PRO_0000425273"
SQ   SEQUENCE   441 AA;  50306 MW;  937931BFFE8061E2 CRC64;
     MRIGLFTDTY FPQVSGVATS IRTLKTELEK QGHAVFIFTT TDKDVNRYED WQIIRIPSVP
     FFAFKDRRFA YRGFSKALEI AKQYQLDIIH TQTEFSLGLL GIWIARELKI PVIHTYHTQY
     EDYVHYIAKG MLIRPSMVKY LVRGFLHDVD GVICPSEIVR DLLSDYKVKV EKRVIPTGIE
     LAKFERPEIK QENLKELRSK LGIQDGEKTL LSLSRISYEK NIQAVLVAFA DVLKEEDKVK
     LVVAGDGPYL NDLKEQAQNL EIQDSVIFTG MIAPSETALY YKAADFFISA STSETQGLTY
     LESLASGTPV IAHGNPYLNN LISDKMFGAL YYGEHDLAGA ILEALIATPD MNEHTLSEKL
     YEISAENFGK RVHEFYLDAI ISNNFQKDLA KDDTVSQRIF KTVLYLPQQV VAVPVKGSRR
     MLKASKTQLI SMRDYWKDHE E