AMER3_HUMAN
ID AMER3_HUMAN Reviewed; 861 AA.
AC Q8N944; B7ZLH6;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=APC membrane recruitment protein 3;
DE Short=Amer3;
DE AltName: Full=Protein FAM123C;
GN Name=AMER3; Synonyms=FAM123C;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT PRO-340.
RC TISSUE=Brain, and Cerebellum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT PRO-340.
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT PRO-340.
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Regulator of the canonical Wnt signaling pathway. Acts by
CC specifically binding phosphatidylinositol 4,5-bisphosphate
CC (PtdIns(4,5)P2), translocating to the cell membrane (By similarity).
CC {ECO:0000250}.
CC -!- INTERACTION:
CC Q8N944; Q5JTC6: AMER1; NbExp=4; IntAct=EBI-8869590, EBI-6169747;
CC Q8N944; P25054: APC; NbExp=8; IntAct=EBI-8869590, EBI-727707;
CC Q8N944; Q9Y2T1: AXIN2; NbExp=2; IntAct=EBI-8869590, EBI-4400025;
CC Q8N944; O88566: Axin2; Xeno; NbExp=2; IntAct=EBI-8869590, EBI-7690990;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC protein {ECO:0000250}. Note=Translocates to the cell membrane following
CC binding to PtdIns(4,5)P2. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Amer family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK095696; BAC04612.1; -; mRNA.
DR EMBL; AK289525; BAF82214.1; -; mRNA.
DR EMBL; AK291424; BAF84113.1; -; mRNA.
DR EMBL; AC010984; AAY24355.1; -; Genomic_DNA.
DR EMBL; AC140481; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471250; EAW51273.1; -; Genomic_DNA.
DR EMBL; BC113445; AAI13446.1; -; mRNA.
DR EMBL; BC113447; AAI13448.1; -; mRNA.
DR EMBL; BC143806; AAI43807.1; -; mRNA.
DR CCDS; CCDS2164.1; -.
DR RefSeq; NP_001098663.1; NM_001105193.1.
DR RefSeq; NP_001098664.1; NM_001105194.1.
DR RefSeq; NP_001098665.1; NM_001105195.1.
DR RefSeq; NP_689911.2; NM_152698.2.
DR RefSeq; XP_011509095.1; XM_011510793.2.
DR AlphaFoldDB; Q8N944; -.
DR BioGRID; 128492; 1.
DR IntAct; Q8N944; 4.
DR MINT; Q8N944; -.
DR STRING; 9606.ENSP00000392700; -.
DR iPTMnet; Q8N944; -.
DR PhosphoSitePlus; Q8N944; -.
DR BioMuta; AMER3; -.
DR DMDM; 311033381; -.
DR MassIVE; Q8N944; -.
DR PaxDb; Q8N944; -.
DR PeptideAtlas; Q8N944; -.
DR PRIDE; Q8N944; -.
DR ProteomicsDB; 72485; -.
DR Antibodypedia; 50181; 18 antibodies from 7 providers.
DR DNASU; 205147; -.
DR Ensembl; ENST00000321420.5; ENSP00000314914.4; ENSG00000178171.11.
DR Ensembl; ENST00000423981.1; ENSP00000392700.1; ENSG00000178171.11.
DR GeneID; 205147; -.
DR KEGG; hsa:205147; -.
DR MANE-Select; ENST00000321420.5; ENSP00000314914.4; NM_152698.3; NP_689911.2.
DR UCSC; uc002trw.3; human.
DR CTD; 205147; -.
DR DisGeNET; 205147; -.
DR GeneCards; AMER3; -.
DR HGNC; HGNC:26771; AMER3.
DR HPA; ENSG00000178171; Tissue enriched (brain).
DR neXtProt; NX_Q8N944; -.
DR OpenTargets; ENSG00000178171; -.
DR PharmGKB; PA162385791; -.
DR VEuPathDB; HostDB:ENSG00000178171; -.
DR eggNOG; ENOG502QVAK; Eukaryota.
DR GeneTree; ENSGT00530000063529; -.
DR HOGENOM; CLU_011805_0_0_1; -.
DR InParanoid; Q8N944; -.
DR OMA; LSMCSFH; -.
DR OrthoDB; 152946at2759; -.
DR PhylomeDB; Q8N944; -.
DR TreeFam; TF333006; -.
DR PathwayCommons; Q8N944; -.
DR SignaLink; Q8N944; -.
DR BioGRID-ORCS; 205147; 18 hits in 1065 CRISPR screens.
DR GenomeRNAi; 205147; -.
DR Pharos; Q8N944; Tdark.
DR PRO; PR:Q8N944; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q8N944; protein.
DR Bgee; ENSG00000178171; Expressed in cortical plate and 56 other tissues.
DR ExpressionAtlas; Q8N944; baseline and differential.
DR Genevisible; Q8N944; HS.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0008013; F:beta-catenin binding; IBA:GO_Central.
DR GO; GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IBA:GO_Central.
DR GO; GO:0060828; P:regulation of canonical Wnt signaling pathway; IBA:GO_Central.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR019003; AMER.
DR PANTHER; PTHR22237; PTHR22237; 1.
DR Pfam; PF09422; WTX; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Lipid-binding; Membrane; Reference proteome;
KW Wnt signaling pathway.
FT CHAIN 1..861
FT /note="APC membrane recruitment protein 3"
FT /id="PRO_0000320594"
FT REGION 1..77
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 179..206
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 261..289
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 351..415
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 514..558
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 576..644
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 716..742
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 786..822
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 264..278
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 362..388
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 517..534
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 611..637
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 717..742
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 340
FT /note="S -> P (in dbSNP:rs1905235)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334, ECO:0000269|PubMed:15815621"
FT /id="VAR_039218"
SQ SEQUENCE 861 AA; 90445 MW; A98BF11AB57E1E12 CRC64;
MELKRGKTFI KSSLQVSHEK PPDPAAVAAA REGTGPWSVL PGGQQRPHSE KGPQASPSAQ
EYDRCPNKGA QLDPKGGPAA LCGATFKPVR KCKTHDSMSG AGRATAATGQ LVGSASFPGS
PGSRRMIDYR HFVPQMPFVP AVAKSIPRKR ISLKRPKKCF RNLFHIRRNK TEDLASLAAE
GKSLPSPGDP SDPGGRRSKA FLPPGEGPGL DGLCQDLLDS ELLADASFGL CRALCEDVAS
LQSFDSLTGC GEVFADESSV PSLELNEGPE SPTQAAQGLE SKVPRGPLQG SVEQLASPAQ
NEASDFTRFW DSVNRSVRQQ QRALLGPWLS GPQGTDRDQS RLDTAGLAEL PLCPCRDPRS
GSKASSIDTG TPKSEQPESV STSDEGYYDS FSPGLEEDKK EAESPGTPAA TFPRDSYSGD
ALYELFHDPS EGPLGPSPDD DLCVSESLSG PALGTPLSIC SFRVGAEENL APAPGPDLLS
QGFLQSSWKG KECLLKLCDT ELAITMGIVS WLRRGPTPRA PPTPGQPAAP PGSQGAPRAP
TEKLGGREGL ASDAGGATVC SAPSRQELWA HPGTTGLLAG ESKALGGATQ GTGTLSRDAS
REEETRGHSE GLFSSMESAA TSTTDTSGKN KAPVPSTWPC SQKEPGPPGV LGCFRGPWRP
GHGGDTLDAE PMLAGCVARV AALKISSNEQ PPAAWPPRQD MGSGLFGQRW ARGPDMLEQK
QSSSSPSMTT IHGLPYSAST QDQRCRDRVQ DLSWLRVEPT GLGVQAWASV EDQPLQLSTE
AVEQVAHGSQ LDSEPRSAPA ARWSSQGHHP ESLGLTLNSQ QEGGVSASAP ECRCSLLARE
GLLCGQPEVG ASGPAMAEPH L
