GLOX_ARTAN
ID GLOX_ARTAN Reviewed; 594 AA.
AC A0A2H4HHY6;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 28-FEB-2018, sequence version 1.
DT 03-AUG-2022, entry version 12.
DE RecName: Full=Putative aldehyde oxidase Art an 7 {ECO:0000305};
DE EC=1.2.3.1 {ECO:0000305};
DE AltName: Full=Allergen Art an 7 {ECO:0000303|PubMed:29220102, ECO:0000303|PubMed:30155917};
DE Short=AN70 {ECO:0000303|PubMed:29220102};
DE AltName: Allergen=Art an 7.0101 {ECO:0000305};
DE Flags: Precursor;
OS Artemisia annua (Sweet wormwood).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Asteroideae; Anthemideae;
OC Artemisiinae; Artemisia.
OX NCBI_TaxID=35608 {ECO:0000312|EMBL:ARQ16437.1};
RN [1] {ECO:0000312|EMBL:ARQ16437.1}
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 107-135; 176-252; 278-287;
RP 291-343; 349-504 AND 511-545, IDENTIFICATION BY MASS SPECTROMETRY,
RP FUNCTION, TISSUE SPECIFICITY, PTM, MASS SPECTROMETRY, ALLERGEN, AND
RP 3D-STRUCTURE MODELING.
RC TISSUE=Pollen {ECO:0000312|EMBL:ARQ16437.1};
RX PubMed=29220102; DOI=10.1111/all.13375;
RA Fu W., Gao Z., Gao L., Jin J., Liu M., Sun Y., Wu S., Wu L., Ma H.,
RA Dong Y., Wang X., Gao B., Wang H., Akkerdaas J.H., Versteeg S.A.,
RA van Ree R.;
RT "Identification of a 62-kDa major allergen from Artemisia pollen as a
RT putative galactose oxidase.";
RL Allergy 73:1041-1052(2018).
RN [2]
RP ALLERGEN.
RX PubMed=30155917; DOI=10.1111/all.13597;
RA Gao Z., Fu W.Y., Sun Y., Gao B., Wang H.Y., Liu M., Luo F.M., Zhou X.,
RA Jin J., Zhao L., Wu S., Liu Y., Wu L., Wang X., Tang N.B., Guo B.H.,
RA Feng Y., Zhou J.Y., Gadermaier G., Ferreira F., Versteeg S.A., van Ree R.;
RT "Artemisia pollen allergy in China: Component-resolved diagnosis reveals
RT allergic asthma patients have significant multiple allergen
RT sensitization.";
RL Allergy 74:284-293(2019).
RN [3]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=30970365; DOI=10.1159/000497321;
RA Gao Z.S., Fu W.Y., Zhao L., Gao L., Zhou J.Y., Gao B.Y., Wu S.,
RA Versteeg S.A., Ferreira F., Gadermaier G., van Ree R.;
RT "Localization of Four Allergens in Artemisia Pollen by Immunofluorescent
RT Antibodies.";
RL Int. Arch. Allergy Immunol. 179:165-172(2019).
CC -!- FUNCTION: Catalyzes the oxidation of aldehydes to the corresponding
CC carboxylate by coupling the reaction to the reduction of dioxygen to
CC hydrogen peroxide. Substrates include glyoxal and other aldehydes
CC (Probable). Does not have enzymatic activity on D-galactose
CC (PubMed:29220102). {ECO:0000269|PubMed:29220102, ECO:0000305}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an aldehyde + H2O + O2 = a carboxylate + H(+) + H2O2;
CC Xref=Rhea:RHEA:16829, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:17478,
CC ChEBI:CHEBI:29067; EC=1.2.3.1; Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:30970365}.
CC Note=Localized around nucleus. {ECO:0000269|PubMed:30970365}.
CC -!- TISSUE SPECIFICITY: Expressed in pollen (at protein level).
CC {ECO:0000269|PubMed:29220102, ECO:0000269|PubMed:30970365}.
CC -!- PTM: The N-terminus is blocked. {ECO:0000269|PubMed:29220102}.
CC -!- PTM: Glycosylated. {ECO:0000269|PubMed:29220102}.
CC -!- MASS SPECTROMETRY: Mass=61954.35; Method=MALDI; Note=The measured mass
CC is that of the mature protein.; Evidence={ECO:0000269|PubMed:29220102};
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE of
CC patients allergic to mugwort pollen (PubMed:29220102, PubMed:30155917).
CC The natural protein binds to IgE in 94% and 95% of the 113 Chinese and
CC 20 Dutch patients tested, respectively. The natural protein gives a
CC positive reaction in 50% of the 14 Chinese patients tested by
CC intradermal skin testing. All except one of these positives are also
CC positive by ImmunoCAP test. The recombinant protein binds to IgE in 4%
CC and 50% of the 54 Chinese and 20 Dutch patients tested, respectively.
CC The lack of IgE-binding to the recombinant protein is not due to the
CC absence of cross-reactive carbohydrate determinants (CCD), although in
CC a subset of (Dutch) sera CCD does seem to play a role in the IgE
CC recognition (PubMed:29220102). Binds to IgE in 80% of the 240 Chinese
CC patients tested. Adult group has a significantly lower IgE level than
CC the child and teenager group. The patients from Southwestern China have
CC significantly lower frequency of sensitization and lower IgE levels
CC against this protein than the patients from Northern China. Patients
CC allergic to multiple Artemisia allergens, including Art an 7, Art v 1
CC and Art v 3, have a higher risk of developing allergic asthma
CC (PubMed:30155917). {ECO:0000269|PubMed:29220102,
CC ECO:0000269|PubMed:30155917}.
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DR EMBL; KY428925; ARQ16437.1; -; mRNA.
DR AlphaFoldDB; A0A2H4HHY6; -.
DR SMR; A0A2H4HHY6; -.
DR Allergome; 12010; Art an 7.
DR Allergome; 12011; Art an 7.0101.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0004031; F:aldehyde oxidase activity; IEA:UniProtKB-EC.
DR CDD; cd02851; E_set_GO_C; 1.
DR Gene3D; 2.130.10.80; -; 1.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR011043; Gal_Oxase/kelch_b-propeller.
DR InterPro; IPR037293; Gal_Oxidase_central_sf.
DR InterPro; IPR009880; Glyoxal_oxidase_N.
DR InterPro; IPR015202; GO-like_E_set.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR Pfam; PF09118; DUF1929; 1.
DR Pfam; PF07250; Glyoxal_oxid_N; 1.
DR SUPFAM; SSF50965; SSF50965; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
PE 1: Evidence at protein level;
KW Allergen; Cytoplasm; Direct protein sequencing; Glycoprotein;
KW Oxidoreductase; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..594
FT /note="Putative aldehyde oxidase Art an 7"
FT /id="PRO_5014160613"
FT REGION 28..56
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 594 AA; 64526 MW; 2A2EED743A07093D CRC64;
MASSIKTVIL FLLPLLLAYS VLAAPDITDG GDKPGPEIDD GGGDKPVPGN NDGASDYAKP
AFEPEFMGAW VIDNPNAGVA AMQLQLMPND QIVWFDTTSL GASGYKLPEG TPCPINPDAN
NQPDCYAHGI AYDWKTSKYR PLTLQGDAWC SSGNLWPNGN LMATGGTFSG DKAIRVIAND
DPKGDFTTKI GALADTRWYS SNQVLPDGSS VVLGGRDSYS YEIVPPQMEF KPKRFDLPFM
QQTTEPPLGP GRPVENNLYP FLFLLPDGNI FLFANNRAIT FEPATGKTVK EFPVLPGGSR
NYPPSGSAAL FPLKLTADNA PVIPEIVICG GNQPNAYELV DARHVTEKQF LPALQDCNRI
QPMAADAAWI PEQNMPSPRT MGDLIHLANG DMLMLNGAKK GTSGWEDATE ANLTPVLYTP
YKPMGQRFKE LTPTTIARMY HSCSALLPDT RVLVAGSNMH QFYTFDTEFP TELRVEKFSP
PYLDPALETE RTQIDPANTD AVLKYGKPFK ITAALMEKQP LVLGEVKVTL LYPPFTTHGF
SQNQRMIVPA ITSVQNGVIT AVAPPSGQIA PPGYYIMFVS HLGIPGAGIW VHID
