GLN13_ORYSJ
ID GLN13_ORYSJ Reviewed; 370 AA.
AC Q4W8D0; Q10DZ8; Q9AUQ3;
DT 13-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 1.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Glutamine synthetase cytosolic isozyme 1-3 {ECO:0000305};
DE EC=6.3.1.2 {ECO:0000250|UniProtKB:P14656};
DE AltName: Full=Glutamate--ammonia ligase GLN1;3 {ECO:0000305};
DE Short=OsGLN1;3 {ECO:0000305};
DE AltName: Full=OsGS1;3 {ECO:0000303|PubMed:15918879};
GN Name=GLN1-3 {ECO:0000305};
GN OrderedLocusNames=Os03g0712800 {ECO:0000312|EMBL:BAS86052.1},
GN LOC_Os03g50490 {ECO:0000312|EMBL:ABF98528.1};
GN ORFNames=OsJ_12330 {ECO:0000312|EMBL:EEE59806.1}, OSJNBb0033N16.19;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Sasanishiki; TISSUE=Flower;
RX PubMed=15918879; DOI=10.1111/j.1365-313x.2005.02406.x;
RA Tabuchi M., Sugiyama K., Ishiyama K., Inoue E., Sato T., Takahashi H.,
RA Yamaya T.;
RT "Severe reduction in growth rate and grain filling of rice mutants lacking
RT OsGS1;1, a cytosolic glutamine synthetase1;1.";
RL Plant J. 42:641-651(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Glutamine synthetase involved in ammonium assimilation.
CC {ECO:0000250|UniProtKB:P14656}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-glutamate + NH4(+) = ADP + H(+) + L-glutamine +
CC phosphate; Xref=Rhea:RHEA:16169, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:28938, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:456216; EC=6.3.1.2;
CC Evidence={ECO:0000250|UniProtKB:P14656};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16170;
CC Evidence={ECO:0000250|UniProtKB:P14656};
CC -!- SUBUNIT: Homooctamer. {ECO:0000250|UniProtKB:P16580}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in spikelets (rice flower).
CC {ECO:0000269|PubMed:15918879}.
CC -!- SIMILARITY: Belongs to the glutamine synthetase family. {ECO:0000305}.
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DR EMBL; AB180689; BAD99301.1; -; mRNA.
DR EMBL; AC082645; AAK18848.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF98528.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF12981.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS86052.1; -; Genomic_DNA.
DR EMBL; CM000140; EEE59806.1; -; Genomic_DNA.
DR EMBL; AK063913; BAG88908.1; -; mRNA.
DR EMBL; AK099290; BAG94045.1; -; mRNA.
DR RefSeq; XP_015628694.1; XM_015773208.1.
DR AlphaFoldDB; Q4W8D0; -.
DR SMR; Q4W8D0; -.
DR STRING; 4530.OS03T0712800-02; -.
DR PaxDb; Q4W8D0; -.
DR PRIDE; Q4W8D0; -.
DR EnsemblPlants; Os03t0712800-02; Os03t0712800-02; Os03g0712800.
DR GeneID; 4333896; -.
DR Gramene; Os03t0712800-02; Os03t0712800-02; Os03g0712800.
DR KEGG; osa:4333896; -.
DR eggNOG; KOG0683; Eukaryota.
DR HOGENOM; CLU_036762_1_1_1; -.
DR InParanoid; Q4W8D0; -.
DR OMA; EVEEPWF; -.
DR OrthoDB; 784869at2759; -.
DR BRENDA; 6.3.1.2; 8948.
DR PlantReactome; R-OSA-1119291; Nitrate assimilation.
DR PlantReactome; R-OSA-1119293; Glutamine biosynthesis I.
DR PlantReactome; R-OSA-1119443; Ammonia assimilation cycle.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q4W8D0; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004356; F:glutamate-ammonia ligase activity; IBA:GO_Central.
DR GO; GO:0006542; P:glutamine biosynthetic process; IBA:GO_Central.
DR Gene3D; 3.10.20.70; -; 1.
DR InterPro; IPR008147; Gln_synt_b-grasp.
DR InterPro; IPR036651; Gln_synt_N.
DR InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR InterPro; IPR008146; Gln_synth_cat_dom.
DR InterPro; IPR027303; Gln_synth_gly_rich_site.
DR InterPro; IPR027302; Gln_synth_N_conserv_site.
DR Pfam; PF00120; Gln-synt_C; 1.
DR Pfam; PF03951; Gln-synt_N; 1.
DR SMART; SM01230; Gln-synt_C; 1.
DR SUPFAM; SSF54368; SSF54368; 1.
DR SUPFAM; SSF55931; SSF55931; 1.
DR PROSITE; PS00180; GLNA_1; 1.
DR PROSITE; PS00181; GLNA_ATP; 1.
DR PROSITE; PS51986; GS_BETA_GRASP; 1.
DR PROSITE; PS51987; GS_CATALYTIC; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cytoplasm; Ligase; Nitrogen fixation; Nucleotide-binding;
KW Reference proteome.
FT CHAIN 1..370
FT /note="Glutamine synthetase cytosolic isozyme 1-3"
FT /id="PRO_0000239822"
FT DOMAIN 21..101
FT /note="GS beta-grasp"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01330"
FT DOMAIN 108..370
FT /note="GS catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01331"
FT REGION 41..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 370 AA; 40678 MW; 5A0B525D2F78CD23 CRC64;
MSSSLLTDLV NLDLSESTDK VIAEYIWVGG TGMDVRSKAR TLSGPVDDPS KLPKWNFDGS
STGQATGDDS EVILHPQAIF RDPFRKGKNI LVMCDCYAPN GEPIPTNNRY NAARIFSHPD
VKAEEPWYGI EQEYTLLQKH INWPLGWPLG GYPGPQGPYY CAAGADKSYG RDIVDAHYKA
CLFAGINISG INAEVMPGQW EFQIGPVVGV SAGDHVWVAR YILERITEIA GVVVSFDPKP
IPGDWNGAGA HTNYSTKSMR SNGGYEVIKK AIKKLGMRHR EHIAAYGDGN ERRLTGRHET
ADINNFVWGV ANRGASVRVG RDTEKDGKGY FEDRRPASNM DPYLVTAMIA ETTILWEPSH
GHGHGQSNGK
