GILT2_DROME
ID GILT2_DROME Reviewed; 207 AA.
AC Q9VCK1;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=GILT-like protein 2 {ECO:0000305};
DE EC=1.8.-.- {ECO:0000250|UniProtKB:P13284};
DE Flags: Precursor;
GN Name=GILT2 {ECO:0000312|FlyBase:FBgn0039099};
GN ORFNames=CG10157 {ECO:0000312|FlyBase:FBgn0039099};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAM12281.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM12281.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAM12281.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24491521; DOI=10.1016/j.dci.2014.01.007;
RA Kongton K., McCall K., Phongdara A.;
RT "Identification of gamma-interferon-inducible lysosomal thiol reductase
RT (GILT) homologues in the fruit fly Drosophila melanogaster.";
RL Dev. Comp. Immunol. 44:389-396(2014).
CC -!- FUNCTION: Probable lysosomal thiol reductase that can reduce protein
CC disulfide bonds (By similarity). Involved in the immune response to
CC bacterial infection (PubMed:24491521). {ECO:0000250|UniProtKB:P13284,
CC ECO:0000269|PubMed:24491521}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- INDUCTION: Up-regulated following injection with the Gram-negative
CC bacterium E.coli. Up-regulation increases between 1 and 12 hours after
CC the injection, then decreases between 12 and 48 hours, and then
CC increases again at 72 hours. {ECO:0000269|PubMed:24491521}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown in the fat body or
CC hemocyte of flies infected with the Gram-negative bacterium E.coli
CC results in an increase in bacterial load 24 hours after infection.
CC {ECO:0000269|PubMed:24491521}.
CC -!- SIMILARITY: Belongs to the GILT family. {ECO:0000305}.
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DR EMBL; AE014297; AAF56157.1; -; Genomic_DNA.
DR EMBL; AY095188; AAM12281.1; -; mRNA.
DR RefSeq; NP_651166.1; NM_142909.4.
DR AlphaFoldDB; Q9VCK1; -.
DR SMR; Q9VCK1; -.
DR IntAct; Q9VCK1; 3.
DR STRING; 7227.FBpp0083827; -.
DR GlyGen; Q9VCK1; 1 site.
DR PaxDb; Q9VCK1; -.
DR PRIDE; Q9VCK1; -.
DR DNASU; 42788; -.
DR EnsemblMetazoa; FBtr0084435; FBpp0083827; FBgn0039099.
DR GeneID; 42788; -.
DR KEGG; dme:Dmel_CG10157; -.
DR UCSC; CG10157-RA; d. melanogaster.
DR CTD; 42788; -.
DR FlyBase; FBgn0039099; GILT2.
DR VEuPathDB; VectorBase:FBgn0039099; -.
DR eggNOG; KOG3160; Eukaryota.
DR GeneTree; ENSGT00940000173058; -.
DR HOGENOM; CLU_066886_2_2_1; -.
DR InParanoid; Q9VCK1; -.
DR OMA; FCAKYKE; -.
DR OrthoDB; 803513at2759; -.
DR PhylomeDB; Q9VCK1; -.
DR Reactome; R-DME-2132295; MHC class II antigen presentation.
DR SignaLink; Q9VCK1; -.
DR BioGRID-ORCS; 42788; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 42788; -.
DR PRO; PR:Q9VCK1; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0039099; Expressed in seminal fluid secreting gland and 24 other tissues.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0016667; F:oxidoreductase activity, acting on a sulfur group of donors; ISS:FlyBase.
DR GO; GO:0016671; F:oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor; IEA:InterPro.
DR GO; GO:0002376; P:immune system process; IEA:UniProtKB-KW.
DR GO; GO:1900426; P:positive regulation of defense response to bacterium; IMP:FlyBase.
DR InterPro; IPR004911; Interferon-induced_GILT.
DR InterPro; IPR036249; Thioredoxin-like_sf.
DR PANTHER; PTHR13234; PTHR13234; 1.
DR Pfam; PF03227; GILT; 1.
DR SUPFAM; SSF52833; SSF52833; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Immunity; Oxidoreductase;
KW Redox-active center; Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..207
FT /note="GILT-like protein 2"
FT /evidence="ECO:0000255"
FT /id="PRO_5008180644"
FT CARBOHYD 182
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 40..43
FT /note="Redox-active"
FT /evidence="ECO:0000250|UniProtKB:P13284"
SQ SEQUENCE 207 AA; 23506 MW; D87B6A3BCBC2040B CRC64;
MRAAVFVCLL LGWVGVATPR RLRGPQADRL AITLYYEALC PYCMEFVTTQ LNPSMVRQDR
LPFTDLTLVP YGNARTNDDG NVECQHGVME CELNAWHACI LEHHDIAQSL KLIACMMRGK
KNRLEKCADH YQIDVGDVKN CKKTRQVNDI LRKYGKETAK VSFQGVPAVA LDNVYNADLS
ANLTDHFDAI FCAKYKEKFN KQLNNCQ
