ID   GIGA2_CRAGI             Reviewed;         340 AA.
AC   P86785;
DT   11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT   11-JAN-2011, sequence version 1.
DT   03-AUG-2022, entry version 20.
DE   RecName: Full=Gigasin-2 {ECO:0000303|Ref.2};
DE   Flags: Precursor;
OS   Crassostrea gigas (Pacific oyster) (Crassostrea angulata).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Bivalvia;
OC   Autobranchia; Pteriomorphia; Ostreida; Ostreoidea; Ostreidae; Crassostrea.
OX   NCBI_TaxID=29159;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=18054177; DOI=10.1016/j.gene.2007.10.021;
RA   Tanguy A., Bierne N., Saavedra C., Pina B., Bachere E., Kube M., Bazin E.,
RA   Bonhomme F., Boudry P., Boulo V., Boutet I., Cancela L., Dossat C.,
RA   Favrel P., Huvet A., Jarque S., Jollivet D., Klages S., Lapegue S.,
RA   Leite R., Moal J., Moraga D., Reinhardt R., Samain J.F., Zouros E.,
RA   Canario A.;
RT   "Increasing genomic information in bivalves through new EST collections in
RT   four species: development of new genetic markers for environmental studies
RT   and genome evolution.";
RL   Gene 408:27-36(2008).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 50-59; 137-143; 212-221 AND 287-297, AND TISSUE
RP   SPECIFICITY.
RC   TISSUE=Shell {ECO:0000269|Ref.2};
RA   Marie B., Zanella-Cleon I., Becchi M., Marin F.;
RT   "Proteomic identification of novel proteins from the calcifying shell
RT   matrix of the Pacific oyster Crassostrea gigas.";
RL   FEBS Lett. 0:0-0(2010).
CC   -!- TISSUE SPECIFICITY: Component of the organic matrix of calcified shell
CC       layers. {ECO:0000269|Ref.2}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AM868448; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AM864770; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; AM868448; -; NOT_ANNOTATED_CDS; mRNA.
DR   PRIDE; P86785; -.
DR   HOGENOM; CLU_746501_0_0_1; -.
DR   Proteomes; UP000005408; Unassembled WGS sequence.
DR   InterPro; IPR000742; EGF-like_dom.
DR   SMART; SM00181; EGF; 2.
DR   PROSITE; PS00022; EGF_1; 2.
DR   PROSITE; PS01186; EGF_2; 2.
DR   PROSITE; PS50026; EGF_3; 2.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; EGF-like domain;
KW   Reference proteome; Repeat; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..340
FT                   /note="Gigasin-2"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000403307"
FT   DOMAIN          22..57
FT                   /note="EGF-like 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DOMAIN          65..97
FT                   /note="EGF-like 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        25..39
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        33..45
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        47..56
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        69..79
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        73..85
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT   DISULFID        87..96
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
SQ   SEQUENCE   340 AA;  37687 MW;  DD9CEFB9FB186EF1 CRC64;
     MNKMSPLYVL ALCCLATTVF AKYDCTNNGG YGCKYGGTCH FYGFCICPKG FQGEDCGLKT
     ELISTAANCT AECKNGGTCY ESDRCYCPHG FIGDMCEIPD TVARCAPDRM IIEAYRPLGF
     VGEVYMFQNR KSCALKQVPS DIRDMMKLER VVLHSDKTEC ALTRTPDTPK LGDVTMKTTV
     VSTHNYNQFG PRDSIMDVSC VHTNSFQGST KEITETAFPF RMVALDMNGE PVQALAANES
     IILQFEPVGI PDVRGVMVEY LEVYSINANS NEVVSKTIIE NGCVLRTAQQ HLEIPIRNYS
     EMXRQGTSWV ARSAMRAFIL LPGDHCYSSL DYASVPEVPR