ID   GID8_DICDI              Reviewed;         228 AA.
AC   Q54X16;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   28-JUL-2009, sequence version 2.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Glucose-induced degradation protein 8 homolog;
GN   ORFNames=DDB_G0279265;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Core component of the CTLH E3 ubiquitin-protein ligase
CC       complex that mediates ubiquitination and subsequent proteasomal
CC       degradation of target proteins. Acts as a positive regulator of Wnt
CC       signaling pathway by promoting beta-catenin (CTNNB1) nuclear
CC       accumulation. {ECO:0000250|UniProtKB:Q9NWU2}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9NWU2}. Nucleus
CC       {ECO:0000250|UniProtKB:Q9NWU2}.
CC   -!- SIMILARITY: Belongs to the GID8 family. {ECO:0000305}.
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DR   EMBL; AAFI02000030; EAL67781.2; -; Genomic_DNA.
DR   RefSeq; XP_641761.2; XM_636669.2.
DR   AlphaFoldDB; Q54X16; -.
DR   SMR; Q54X16; -.
DR   STRING; 44689.DDB0305011; -.
DR   PaxDb; Q54X16; -.
DR   EnsemblProtists; EAL67781; EAL67781; DDB_G0279265.
DR   GeneID; 8621959; -.
DR   KEGG; ddi:DDB_G0279265; -.
DR   dictyBase; DDB_G0279265; -.
DR   eggNOG; KOG2659; Eukaryota.
DR   HOGENOM; CLU_073203_1_0_1; -.
DR   InParanoid; Q54X16; -.
DR   OMA; QNGRIQE; -.
DR   PhylomeDB; Q54X16; -.
DR   PRO; PR:Q54X16; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   InterPro; IPR013144; CRA_dom.
DR   InterPro; IPR024964; CTLH/CRA.
DR   InterPro; IPR006595; CTLH_C.
DR   InterPro; IPR006594; LisH.
DR   Pfam; PF10607; CLTH; 1.
DR   Pfam; PF08513; LisH; 1.
DR   SMART; SM00757; CRA; 1.
DR   SMART; SM00668; CTLH; 1.
DR   SMART; SM00667; LisH; 1.
DR   PROSITE; PS50897; CTLH; 1.
DR   PROSITE; PS50896; LISH; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Nucleus; Reference proteome; Wnt signaling pathway.
FT   CHAIN           1..228
FT                   /note="Glucose-induced degradation protein 8 homolog"
FT                   /id="PRO_0000328507"
FT   DOMAIN          29..61
FT                   /note="LisH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT   DOMAIN          67..124
FT                   /note="CTLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00058"
SQ   SEQUENCE   228 AA;  25572 MW;  0BD835D2B9F0FEB7 CRC64;
     MSNSYSSNAQ KKVISTSEWD DKLAEVNISK SDLNKLVMNY LVIEGYQEAA AKFQEESSTQ
     TTVDLASIAD RMAIRSAIQC GDVEKGIEIV NDLNPEILDT NPQLYFHLQQ QKLIELIRKG
     MTAEALKFAQ DELAPQGEEN NKFLEELEKT ISLLVFEDTA KSPLSSLLDH SQRQKTAGEL
     NSAILLSQSQ DKDPKLPTIL KLLKWAQTQL DSKCIYPKIT NTVTGEYE