ID   GID8B_DANRE             Reviewed;         229 AA.
AC   E7FGY2; F8W234;
DT   18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT   20-JAN-2016, sequence version 2.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=Glucose-induced degradation protein 8-B homolog;
DE   AltName: Full=Two hybrid-associated protein 1 with RanBPM {ECO:0000303|PubMed:28829046};
DE            Short=Twa1 {ECO:0000303|PubMed:28829046};
GN   Name=gid8b; Synonyms=c20orf11, twa1 {ECO:0000303|PubMed:28829046};
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX   PubMed=28829046; DOI=10.1038/cr.2017.107;
RA   Lu Y., Xie S., Zhang W., Zhang C., Gao C., Sun Q., Cai Y., Xu Z., Xiao M.,
RA   Xu Y., Huang X., Wu X., Liu W., Wang F., Kang Y., Zhou T.;
RT   "Twa1/Gid8 is a beta-catenin nuclear retention factor in Wnt signaling and
RT   colorectal tumorigenesis.";
RL   Cell Res. 27:1422-1440(2017).
CC   -!- FUNCTION: Core component of the CTLH E3 ubiquitin-protein ligase
CC       complex that selectively accepts ubiquitin from UBE2H and mediates
CC       ubiquitination and subsequent proteasomal degradation of target
CC       proteins (By similarity). Acts as a positive regulator of Wnt signaling
CC       pathway by promoting beta-catenin (CTNNB1) nuclear accumulation (By
CC       similarity). Required for normal Wnt signaling and normal dorsoventral
CC       patterning during embryogenesis (PubMed:28829046).
CC       {ECO:0000250|UniProtKB:Q9NWU2, ECO:0000269|PubMed:28829046}.
CC   -!- SUBUNIT: Identified in the CTLH complex that contains at least MAEA,
CC       RMND5A (or alternatively its paralog RMND5B), GID8, WDR26, and RANBP9
CC       and/or RANBP10. Interacts with CTNNB1. {ECO:0000250|UniProtKB:Q9NWU2}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9NWU2}. Nucleus
CC       {ECO:0000250|UniProtKB:Q9NWU2}. Note=Localizes in the cytoplasm in the
CC       absence of Wnt stimulation and in the nucleus in the presence of Wnt
CC       stimulation. {ECO:0000250|UniProtKB:Q9NWU2}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in the early developmental stages of
CC       embryos. {ECO:0000269|PubMed:28829046}.
CC   -!- DISRUPTION PHENOTYPE: Morpholino knockdown produces morphants which
CC       exhibit loss or reduction of dorsal embryonic structures and Wnt
CC       signaling deficiency. {ECO:0000269|PubMed:28829046}.
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DR   EMBL; BX649300; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CR407550; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; XP_002666524.1; XM_002666478.5.
DR   RefSeq; XP_017209191.1; XM_017353702.1.
DR   RefSeq; XP_017209192.1; XM_017353703.1.
DR   AlphaFoldDB; E7FGY2; -.
DR   SMR; E7FGY2; -.
DR   STRING; 7955.ENSDARP00000054737; -.
DR   PaxDb; E7FGY2; -.
DR   PeptideAtlas; E7FGY2; -.
DR   Ensembl; ENSDART00000054738; ENSDARP00000054737; ENSDARG00000037589.
DR   Ensembl; ENSDART00000148457; ENSDARP00000124870; ENSDARG00000037589.
DR   GeneID; 566067; -.
DR   CTD; 566067; -.
DR   ZFIN; ZDB-GENE-030515-4; gid8b.
DR   GeneTree; ENSGT00390000015162; -.
DR   InParanoid; E7FGY2; -.
DR   OMA; QNGRIQE; -.
DR   OrthoDB; 1365635at2759; -.
DR   TreeFam; TF300176; -.
DR   PRO; PR:E7FGY2; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 23.
DR   Bgee; ENSDARG00000037589; Expressed in gastrula and 35 other tissues.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0048263; P:determination of dorsal identity; IMP:ZFIN.
DR   GO; GO:0090263; P:positive regulation of canonical Wnt signaling pathway; IMP:UniProtKB.
DR   GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR   InterPro; IPR013144; CRA_dom.
DR   InterPro; IPR024964; CTLH/CRA.
DR   InterPro; IPR006595; CTLH_C.
DR   InterPro; IPR006594; LisH.
DR   Pfam; PF10607; CLTH; 1.
DR   Pfam; PF08513; LisH; 1.
DR   SMART; SM00757; CRA; 1.
DR   SMART; SM00668; CTLH; 1.
DR   SMART; SM00667; LisH; 1.
DR   PROSITE; PS50897; CTLH; 1.
DR   PROSITE; PS50896; LISH; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Developmental protein; Nucleus; Reference proteome;
KW   Wnt signaling pathway.
FT   CHAIN           1..229
FT                   /note="Glucose-induced degradation protein 8-B homolog"
FT                   /id="PRO_0000444890"
FT   DOMAIN          26..58
FT                   /note="LisH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT   DOMAIN          64..121
FT                   /note="CTLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00058"
SQ   SEQUENCE   229 AA;  26873 MW;  39A741CEE5F7B7B2 CRC64;
     MMSYAEKPED ITRDEWMEKL NNVHIQRADM NRLIMNYLVT EGFKEAAEKF RMESGIEPSV
     DLDSLDERIK IREMVLKGQI QEAIALINSL HPELLDTNRY LYFHLQQQHL IELIRLRETE
     AALEFAQTQL AEQGEESREC LTEMERTLAL LAFDNPEESP FGDLLNMMQR QKVWSEVNQA
     VLDYENREST PKLAKLLKLL LWAQNELDQK KVKYPKMTDL SKGTIEDPK