ID   GID8A_DANRE             Reviewed;         228 AA.
AC   Q6PC55;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Glucose-induced degradation protein 8-A homolog;
DE   AltName: Full=Two hybrid-associated protein 1 with RanBPM {ECO:0000303|PubMed:28829046};
DE            Short=Twa1 {ECO:0000303|PubMed:28829046};
GN   Name=gid8a; Synonyms=twa1 {ECO:0000303|PubMed:28829046};
GN   ORFNames=zgc:73100;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Eye;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX   PubMed=28829046; DOI=10.1038/cr.2017.107;
RA   Lu Y., Xie S., Zhang W., Zhang C., Gao C., Sun Q., Cai Y., Xu Z., Xiao M.,
RA   Xu Y., Huang X., Wu X., Liu W., Wang F., Kang Y., Zhou T.;
RT   "Twa1/Gid8 is a beta-catenin nuclear retention factor in Wnt signaling and
RT   colorectal tumorigenesis.";
RL   Cell Res. 27:1422-1440(2017).
CC   -!- FUNCTION: Core component of the CTLH E3 ubiquitin-protein ligase
CC       complex that selectively accepts ubiquitin from UBE2H and mediates
CC       ubiquitination and subsequent proteasomal degradation of target
CC       proteins (By similarity). Acts as a positive regulator of Wnt signaling
CC       pathway by promoting beta-catenin (CTNNB1) nuclear accumulation (By
CC       similarity). Required for normal Wnt signaling and normal dorsoventral
CC       patterning during embryogenesis (PubMed:28829046).
CC       {ECO:0000250|UniProtKB:Q9NWU2, ECO:0000269|PubMed:28829046}.
CC   -!- SUBUNIT: Identified in the CTLH complex that contains at least MAEA,
CC       RMND5A (or alternatively its paralog RMND5B), GID8, WDR26, and RANBP9
CC       and/or RANBP10. Interacts with CTNNB1. {ECO:0000250|UniProtKB:Q9NWU2}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in the early developmental stages of
CC       embryos. {ECO:0000269|PubMed:28829046}.
CC   -!- DISRUPTION PHENOTYPE: Morpholino knockdown produces morphants which
CC       exhibit loss or reduction of dorsal embryonic structures and Wnt
CC       signaling deficiency. {ECO:0000269|PubMed:28829046}.
CC   -!- SIMILARITY: Belongs to the GID8 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC059468; AAH59468.1; -; mRNA.
DR   RefSeq; NP_957006.1; NM_200712.1.
DR   RefSeq; XP_017212783.1; XM_017357294.1.
DR   AlphaFoldDB; Q6PC55; -.
DR   SMR; Q6PC55; -.
DR   STRING; 7955.ENSDARP00000109237; -.
DR   PaxDb; Q6PC55; -.
DR   Ensembl; ENSDART00000030920; ENSDARP00000035630; ENSDARG00000022768.
DR   Ensembl; ENSDART00000180284; ENSDARP00000145022; ENSDARG00000022768.
DR   GeneID; 393685; -.
DR   KEGG; dre:393685; -.
DR   CTD; 393685; -.
DR   ZFIN; ZDB-GENE-040426-1669; gid8a.
DR   eggNOG; KOG2659; Eukaryota.
DR   GeneTree; ENSGT00390000015162; -.
DR   HOGENOM; CLU_073203_1_0_1; -.
DR   InParanoid; Q6PC55; -.
DR   OMA; KANYPRI; -.
DR   OrthoDB; 1365635at2759; -.
DR   PhylomeDB; Q6PC55; -.
DR   TreeFam; TF300176; -.
DR   PRO; PR:Q6PC55; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 8.
DR   Bgee; ENSDARG00000022768; Expressed in early embryo and 27 other tissues.
DR   ExpressionAtlas; Q6PC55; baseline.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0048263; P:determination of dorsal identity; IMP:ZFIN.
DR   GO; GO:0090263; P:positive regulation of canonical Wnt signaling pathway; IMP:UniProtKB.
DR   GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR   GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR   InterPro; IPR013144; CRA_dom.
DR   InterPro; IPR024964; CTLH/CRA.
DR   InterPro; IPR006595; CTLH_C.
DR   InterPro; IPR006594; LisH.
DR   Pfam; PF10607; CLTH; 1.
DR   Pfam; PF08513; LisH; 1.
DR   SMART; SM00757; CRA; 1.
DR   SMART; SM00668; CTLH; 1.
DR   SMART; SM00667; LisH; 1.
DR   PROSITE; PS50897; CTLH; 1.
DR   PROSITE; PS50896; LISH; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; Reference proteome; Wnt signaling pathway.
FT   CHAIN           1..228
FT                   /note="Glucose-induced degradation protein 8-A homolog"
FT                   /id="PRO_0000328505"
FT   DOMAIN          25..57
FT                   /note="LisH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT   DOMAIN          63..120
FT                   /note="CTLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00058"
SQ   SEQUENCE   228 AA;  26761 MW;  68B30058CDAB75D1 CRC64;
     MSYSEKPEDI TKEEWMDKLN NVHIQRADMN RLIMNYLVTE GFKEAAEKFR MESGIEPNVD
     LDSLDERIKI REMVLKGQIQ EAIALINSLH PELLDTNRYL YFHLQQQHLI ELIRLRETEA
     ALEFAQSQLA EQGEESRECL TEMERTLALL AFDNPEESPF GDLLNMMQRQ KVWSEVNQAV
     LDYENRESTP KLAKLLKLLL WAQNELDQKK VKYSRMTDLS KGTIEDPK