ID   GIAB_GIAIN              Reviewed;         273 AA.
AC   P15518;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Giardin subunit beta;
DE   AltName: Full=Protein 14B, cytoskeletal;
OS   Giardia intestinalis (Giardia lamblia).
OC   Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX   NCBI_TaxID=5741;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SEQUENCE REVISION TO N-TERMINUS.
RC   STRAIN=Portland-1;
RX   PubMed=7659516; DOI=10.1093/nar/23.15.2945;
RA   Holberton D.V., Marshall J.;
RT   "Analysis of consensus sequence patterns in Giardia cytoskeleton gene
RT   promoters.";
RL   Nucleic Acids Res. 23:2945-2953(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Portland-1;
RX   PubMed=3405761; DOI=10.1093/nar/16.14.7177;
RA   Baker D.A., Holberton D.V., Marshall J.;
RT   "Sequence of a giardin subunit cDNA from Giardia lamblia.";
RL   Nucleic Acids Res. 16:7177-7177(1988).
RN   [3]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=3225852; DOI=10.1016/0022-2836(88)90370-1;
RA   Holberton D.V., Baker D.A., Marshall J.;
RT   "Segmented alpha-helical coiled-coil structure of the protein giardin from
RT   the Giardia cytoskeleton.";
RL   J. Mol. Biol. 204:789-795(1988).
CC   -!- FUNCTION: Giardins are involved in parasite attachment to the
CC       intestinal mucosa and in the cytoskeletal disassembly and reassembly
CC       that marks the transition from infectious trophozoite to transmissible
CC       cyst. They may interact with other cytoskeletal proteins such as
CC       microtubules in the microribbons or crossbridges, to maintain the
CC       integrity of the ventral disk.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Note=Microribbons of
CC       ventral disc.
CC   -!- DOMAIN: Shows an alpha-helical coiled coil structure (35 repeating
CC       heptads) related to the alpha-type fibrous proteins (K-M-E-F class).
CC   -!- SIMILARITY: Belongs to the SF-assemblin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA29154.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=CAA32388.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X85958; CAA59935.1; -; Genomic_DNA.
DR   EMBL; X14185; CAA32388.1; ALT_INIT; mRNA.
DR   EMBL; M36728; AAA29154.1; ALT_INIT; mRNA.
DR   AlphaFoldDB; P15518; -.
DR   PRIDE; P15518; -.
DR   VEuPathDB; GiardiaDB:DHA2_151428; -.
DR   VEuPathDB; GiardiaDB:GL50581_2741; -.
DR   VEuPathDB; GiardiaDB:GL50803_004812; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0005200; F:structural constituent of cytoskeleton; IEA:InterPro.
DR   InterPro; IPR038835; Giardin_beta-like.
DR   InterPro; IPR008374; SF_assemblin/giardin_b.
DR   PANTHER; PTHR37027; PTHR37027; 1.
DR   Pfam; PF06705; SF-assemblin; 1.
DR   PRINTS; PR01799; SFASSEMBLIN.
PE   2: Evidence at transcript level;
KW   Coiled coil; Cytoplasm; Cytoskeleton; Microtubule.
FT   CHAIN           1..273
FT                   /note="Giardin subunit beta"
FT                   /id="PRO_0000221444"
FT   REGION          1..19
FT                   /note="Nonhelical region"
FT   REGION          20..273
FT                   /note="Rod"
FT   COILED          123..175
FT                   /evidence="ECO:0000255"
FT   COILED          211..263
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   273 AA;  31005 MW;  77D19C05B8E9C14B CRC64;
     MSMFTSTRTL TQTMDKPDDL TRSATETAVK LSNMNQRVSR FHDKMENEIE VRRVDDDTRV
     KMIKDAIAHL DRLIQTESRK RQASFEDIRE EVKKSADNMY LTIKEEIDTM AANFRKSLAE
     MGDTLNNVET NLQNQIAIHN DAIAALRKEA LKSLNDLETG IATENAERKK MYDQLNEKVA
     EGFARISAAI EKETIARERA VSAATTEALT NTKLVEKCVN EQLENVASEI RAIQEEIDRE
     KAERKEAEDK IVNTLEDVVS KIQGGLSMVT KHQ