GFI1_DROME
ID GFI1_DROME Reviewed; 541 AA.
AC Q9N658; Q4V6Z2;
DT 05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Zinc finger protein sens;
DE AltName: Full=Protein senseless;
GN Name=sens; Synonyms=Lyra; ORFNames=CG32120;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DNA-BINDING, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=10975525; DOI=10.1016/s0092-8674(00)00040-4;
RA Nolo R., Abbott L.A., Bellen H.J.;
RT "Senseless, a Zn finger transcription factor, is necessary and sufficient
RT for sensory organ development in Drosophila.";
RL Cell 102:349-362(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11709152; DOI=10.1016/s0896-6273(01)00480-9;
RA Frankfort B.J., Nolo R., Zhang Z., Bellen H., Mardon G.;
RT "senseless repression of rough is required for R8 photoreceptor
RT differentiation in the developing Drosophila eye.";
RL Neuron 32:403-414(2001).
RN [6]
RP FUNCTION.
RX PubMed=12782275; DOI=10.1016/s0925-4773(03)00037-6;
RA Srivastava A., Bell J.B.;
RT "Further developmental roles of the vestigial/scalloped transcription
RT complex during wing development in Drosophila melanogaster.";
RL Mech. Dev. 120:587-596(2003).
CC -!- FUNCTION: Transcription factor both necessary and sufficient for proper
CC development of most cell types of the embryonic and adult peripheral
CC nervous system (PNS). Essential component of the proneural Notch
CC signaling pathway required for proper sensory organ precursor (SOP)
CC differentiation. Correct expression requires expression of scalloped
CC (sd). Repression of rough (ro) in R8 photoreceptor is an essential
CC mechanism of R8 cell fate determination. {ECO:0000269|PubMed:10975525,
CC ECO:0000269|PubMed:11709152, ECO:0000269|PubMed:12782275}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10975525}.
CC -!- TISSUE SPECIFICITY: Almost exclusively restricted to precursors and
CC early differentiating cells of the embryonic PNS. Expressed in the R8
CC photoreceptor during third instar eye development beginning within the
CC morphogenetic furrow of the eye imaginal disk.
CC {ECO:0000269|PubMed:10975525, ECO:0000269|PubMed:11709152}.
CC -!- DISRUPTION PHENOTYPE: Mutants abolish the further up-regulation and
CC maintenance of proneural gene expression in the SOPs.
CC {ECO:0000269|PubMed:10975525}.
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DR EMBL; AF212312; AAF32280.1; -; mRNA.
DR EMBL; AF212313; AAF32281.1; -; Genomic_DNA.
DR EMBL; AE014296; AAN11834.1; -; Genomic_DNA.
DR EMBL; BT022164; AAY51558.1; -; mRNA.
DR RefSeq; NP_524818.1; NM_080079.3.
DR AlphaFoldDB; Q9N658; -.
DR SMR; Q9N658; -.
DR BioGRID; 69594; 31.
DR STRING; 7227.FBpp0075596; -.
DR PaxDb; Q9N658; -.
DR DNASU; 45328; -.
DR EnsemblMetazoa; FBtr0075862; FBpp0075596; FBgn0002573.
DR GeneID; 45328; -.
DR KEGG; dme:Dmel_CG32120; -.
DR UCSC; CG32120-RA; d. melanogaster.
DR CTD; 45328; -.
DR FlyBase; FBgn0002573; sens.
DR VEuPathDB; VectorBase:FBgn0002573; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000156166; -.
DR HOGENOM; CLU_030838_0_0_1; -.
DR InParanoid; Q9N658; -.
DR OMA; KFKYERR; -.
DR OrthoDB; 1318335at2759; -.
DR PhylomeDB; Q9N658; -.
DR SignaLink; Q9N658; -.
DR BioGRID-ORCS; 45328; 0 hits in 3 CRISPR screens.
DR ChiTaRS; sens; fly.
DR GenomeRNAi; 45328; -.
DR PRO; PR:Q9N658; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0002573; Expressed in proneural cluster and 47 other tissues.
DR ExpressionAtlas; Q9N658; baseline and differential.
DR Genevisible; Q9N658; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0005700; C:polytene chromosome; IDA:FlyBase.
DR GO; GO:0043425; F:bHLH transcription factor binding; IDA:FlyBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:FlyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0007411; P:axon guidance; IMP:FlyBase.
DR GO; GO:0008407; P:chaeta morphogenesis; IMP:FlyBase.
DR GO; GO:0042051; P:compound eye photoreceptor development; IMP:FlyBase.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:FlyBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0007422; P:peripheral nervous system development; IMP:FlyBase.
DR GO; GO:0046530; P:photoreceptor cell differentiation; IMP:FlyBase.
DR GO; GO:0008594; P:photoreceptor cell morphogenesis; IMP:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:GOC.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007423; P:sensory organ development; IMP:FlyBase.
DR GO; GO:0016360; P:sensory organ precursor cell fate determination; IMP:FlyBase.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR InterPro; IPR036236; Znf_C2H2_sf.
DR InterPro; IPR013087; Znf_C2H2_type.
DR Pfam; PF00096; zf-C2H2; 3.
DR SMART; SM00355; ZnF_C2H2; 4.
DR SUPFAM; SSF57667; SSF57667; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 4.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 4.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Metal-binding; Notch signaling pathway;
KW Nucleus; Reference proteome; Repeat; Sensory transduction; Transcription;
KW Transcription regulation; Vision; Zinc; Zinc-finger.
FT CHAIN 1..541
FT /note="Zinc finger protein sens"
FT /id="PRO_0000046922"
FT ZN_FING 413..435
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 441..463
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 469..491
FT /note="C2H2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 497..520
FT /note="C2H2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 110..216
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 313..334
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 354..412
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 514..541
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 126..151
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 152..213
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 354..403
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 514..529
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 541 AA; 61306 MW; D870578007B55F04 CRC64;
MNHLSPPPSP HSQQPSPAGL GCHGAALDKQ WMQRASAFNT VIASAAAQKL NGRDLPFLYN
PLLYSSALLW PQFLLSSATA LGTPLTPMTP KSPASVVLGQ RDRDFALTPE KEHELQMNNN
NENSKQDYQE QDEDMPLNLS TKERITSDDS NRDQYHSSSN NSSRSSSSSE VEQLHPMTSL
NVTPPPLSAV NLKSSSTPQQ QRQRSQGNII WSPASMCERS ARREQYGLKM EEQGDEEEHQ
VDPIVRKFKY ERRTASISSL QSPISSLSAP ASNAVQDLEF EVAQQQLYAH RSAFMAGLTG
NNLELLTQHL KLKSEQPQQQ QQQHRIKDEQ QQDNRSAAAL MNLVAAAEFG YMRNQHQQPQ
QQQQQQLHHQ QQPQQHQHQQ QHPDSTATDV ARRSSSSSSY QGENEEKRSG RNFQCKQCGK
SFKRSSTLST HLLIHSDTRP YPCQYCGKRF HQKSDMKKHT YIHTGEKPHK CTVCLKAFSQ
SSNLITHMRK HTGYKPFGCH LCDQSFQRKV DLRRHRESRH EEAPPVEDLK PLKMEVSSSS
C
