ALOX2_KOMPC
ID ALOX2_KOMPC Reviewed; 663 AA.
AC F2R038; Q9URI7;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 1.
DT 03-AUG-2022, entry version 47.
DE RecName: Full=Alcohol oxidase 2;
DE Short=AO 2;
DE Short=AOX 2;
DE EC=1.1.3.13;
DE AltName: Full=Methanol oxidase 2;
DE Short=MOX 2;
GN Name=AOX2; OrderedLocusNames=PP7435_Chr4-0863;
OS Komagataella phaffii (strain ATCC 76273 / CBS 7435 / CECT 11047 / NRRL
OS Y-11430 / Wegner 21-1) (Yeast) (Pichia pastoris).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Phaffomycetaceae; Komagataella.
OX NCBI_TaxID=981350;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=NRRL Y-11430;
RX PubMed=2660463; DOI=10.1002/yea.320050306;
RA Koutz P., Davis G.R., Stillman C., Barringer K., Cregg J., Thill G.;
RT "Structural comparison of the Pichia pastoris alcohol oxidase genes.";
RL Yeast 5:167-177(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1;
RX PubMed=21575661; DOI=10.1016/j.jbiotec.2011.04.014;
RA Kueberl A., Schneider J., Thallinger G.G., Anderl I., Wibberg D., Hajek T.,
RA Jaenicke S., Brinkrolf K., Goesmann A., Szczepanowski R., Puehler A.,
RA Schwab H., Glieder A., Pichler H.;
RT "High-quality genome sequence of Pichia pastoris CBS7435.";
RL J. Biotechnol. 154:312-320(2011).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1;
RX PubMed=27388471; DOI=10.1093/femsyr/fow051;
RA Valli M., Tatto N.E., Peymann A., Gruber C., Landes N., Ekker H.,
RA Thallinger G.G., Mattanovich D., Gasser B., Graf A.B.;
RT "Curation of the genome annotation of Pichia pastoris (Komagataella
RT phaffii) CBS7435 from gene level to protein function.";
RL FEMS Yeast Res. 16:0-0(2016).
RN [4]
RP FUNCTION, AND INDUCTION BY METHANOL.
RC STRAIN=ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1;
RX PubMed=2657390; DOI=10.1128/mcb.9.3.1316-1323.1989;
RA Cregg J.M., Madden K.R., Barringer K.J., Thill G.P., Stillman C.A.;
RT "Functional characterization of the two alcohol oxidase genes from the
RT yeast Pichia pastoris.";
RL Mol. Cell. Biol. 9:1316-1323(1989).
CC -!- FUNCTION: Minor isoform of alcohol oxidase, which catalyzes the
CC oxidation of methanol to formaldehyde and hydrogen peroxide, the first
CC step in the methanol utilization pathway of methylotrophic yeasts.
CC {ECO:0000269|PubMed:2657390}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a primary alcohol + O2 = an aldehyde + H2O2;
CC Xref=Rhea:RHEA:19829, ChEBI:CHEBI:15379, ChEBI:CHEBI:15734,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:17478; EC=1.1.3.13;
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC -!- PATHWAY: Energy metabolism; methane degradation.
CC -!- SUBUNIT: Homooctamer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Peroxisome matrix {ECO:0000250}.
CC -!- INDUCTION: Induced by methanol. Subject to strong carbon catabolite
CC repression. {ECO:0000269|PubMed:2657390}.
CC -!- DOMAIN: The C-terminal peroxisomal targeting signal (PTS) is essential
CC for the efficient targeting and import of AOX into peroxisomes via the
CC PTS1 pathway. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the GMC oxidoreductase family. {ECO:0000305}.
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DR EMBL; U96968; AAB57850.1; -; Genomic_DNA.
DR EMBL; FR839631; CCA41016.1; -; Genomic_DNA.
DR AlphaFoldDB; F2R038; -.
DR SMR; F2R038; -.
DR CAZy; AA3; Auxiliary Activities 3.
DR EnsemblFungi; CCA41016; CCA41016; PP7435_CHR4-0863.
DR HOGENOM; CLU_002865_5_1_1; -.
DR BRENDA; 1.1.3.13; 4827.
DR UniPathway; UPA00147; -.
DR Proteomes; UP000006853; Chromosome 4.
DR GO; GO:0005782; C:peroxisomal matrix; IEA:UniProtKB-SubCell.
DR GO; GO:0047639; F:alcohol oxidase activity; IEA:UniProtKB-EC.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0046188; P:methane catabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0015945; P:methanol metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.50.50.60; -; 2.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR012132; GMC_OxRdtase.
DR InterPro; IPR000172; GMC_OxRdtase_N.
DR InterPro; IPR007867; GMC_OxRtase_C.
DR PANTHER; PTHR11552; PTHR11552; 1.
DR Pfam; PF05199; GMC_oxred_C; 1.
DR Pfam; PF00732; GMC_oxred_N; 1.
DR PIRSF; PIRSF000137; Alcohol_oxidase; 1.
DR SUPFAM; SSF51905; SSF51905; 1.
DR PROSITE; PS00623; GMC_OXRED_1; 1.
PE 2: Evidence at transcript level;
KW FAD; Flavoprotein; Methanol utilization; Oxidoreductase; Peroxisome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..663
FT /note="Alcohol oxidase 2"
FT /id="PRO_0000425716"
FT MOTIF 661..663
FT /note="Microbody targeting signal"
FT /evidence="ECO:0000255"
FT ACT_SITE 567
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:E4QP00"
FT BINDING 8..38
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000250"
FT CONFLICT 262
FT /note="K -> T (in Ref. 1; AAB57850)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 663 AA; 73952 MW; 9A9D2C84161991B2 CRC64;
MAIPEEFDIL VLGGGSSGSC IAGRLANLDH SLKVGLIEAG ENNLNNPWVY LPGIYPRNMK
LDSKTASFYT SNPSPHLNGR RAIVPCANIL GGGSSINFMM YTRGSASDYD DFEAEGWKTK
DLLPLMKKTE TYQRACNNPE IHGFEGPIKV SFGNYTYPVC QDFLRATESQ GIPYVDDLED
LVTAHGAEHW LKWINRDTGR RSDSAHAFVH STMRNHDNLY LICNTKVDKI IVEDGRAAAV
RTVPSKPLNA KKPTHKVYRA RKQIVLSCGT ISSPLVLQRS GFGDPIKLRA AGVKPLVNLP
GVGRNFQDHY CFFSPYRIKP QYESFDDFVR GDANIQKKVF DQWYANGTGP LATNGIEAGV
KIRPTPEELS QMDESFQEGY REYFEDKPDK PVMHYSIIAG FFGDHTKIPP GKYMTMFHFL
EYPFSRGSIH ITSPDPYATP DFDPGFMNDE RDMAPMVWSY KKSRETARKM DHFAGEVTSH
HPLFPYSSEA RAYEMDLETS NAYGGPLNLT AGLAHGSWTQ PLKKPAGRNE GHVTSNQVEL
HPDIEYDEED DKAIENYIRE HTETTWHCLG TCSIGPREGS KIVKWGGVLD HRSNVYGVKG
LKVGDLSVCP DNVGCNTYTT ALLIGEKTAT LVGEDLGYTG EALDMTVPQF KLGTYEKTGL
ARF
