ID   ALO3_ACRLO              Reviewed;          36 AA.
AC   P83653;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Antimicrobial peptide Alo-3;
OS   Acrocinus longimanus (Giant harlequin beetle).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Coleoptera; Polyphaga; Cucujiformia;
OC   Chrysomeloidea; Cerambycidae; Lamiinae; Acrocinini; Acrocinus.
OX   NCBI_TaxID=227548 {ECO:0000305};
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, MASS SPECTROMETRY, AND STRUCTURE BY NMR.
RC   TISSUE=Hemolymph;
RX   PubMed=14661954; DOI=10.1021/bi035400o;
RA   Barbault F., Landon C., Guenneugues M., Meyer J.-P., Schott V.,
RA   Dimarcq J.-L., Vovelle F.;
RT   "Solution structure of Alo-3: a new knottin-type antifungal peptide from
RT   the insect Acrocinus longimanus.";
RL   Biochemistry 42:14434-14442(2003).
CC   -!- FUNCTION: Has antifungal activity against C.glabrata.
CC       {ECO:0000269|PubMed:14661954, ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin.
CC   -!- MASS SPECTROMETRY: Mass=3869.4; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:14661954};
CC   -!- SIMILARITY: Belongs to the AMP family. {ECO:0000305}.
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DR   PDB; 1Q3J; NMR; -; A=1-36.
DR   PDBsum; 1Q3J; -.
DR   AlphaFoldDB; P83653; -.
DR   SMR; P83653; -.
DR   EvolutionaryTrace; P83653; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR013006; Antimicrobial_C6_CS.
DR   InterPro; IPR009101; Gurmarin/antifun_pep.
DR   InterPro; IPR024206; Gurmarin/antimicrobial_peptd.
DR   Pfam; PF11410; Antifungal_pept; 1.
DR   SUPFAM; SSF57048; SSF57048; 1.
DR   PROSITE; PS60011; PLANT_C6_AMP; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW   Fungicide; Knottin; Secreted.
FT   PEPTIDE         1..36
FT                   /note="Antimicrobial peptide Alo-3"
FT                   /id="PRO_0000045066"
FT   DISULFID        1..18
FT                   /evidence="ECO:0000305"
FT   DISULFID        8..22
FT                   /evidence="ECO:0000305"
FT   DISULFID        17..33
FT                   /evidence="ECO:0000305"
FT   STRAND          3..11
FT                   /evidence="ECO:0007829|PDB:1Q3J"
FT   STRAND          26..33
FT                   /evidence="ECO:0007829|PDB:1Q3J"
SQ   SEQUENCE   36 AA;  3875 MW;  32685DDBF11ECC1A CRC64;
     CIKNGNGCQP NGSQGNCCSG YCHKQPGWVA GYCRRK