ALNR_EMENI
ID ALNR_EMENI Reviewed; 407 AA.
AC P0CU78; A0A1U8QXS7; C8VJR1; Q5AR59;
DT 05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT 05-DEC-2018, sequence version 1.
DT 25-MAY-2022, entry version 13.
DE RecName: Full=Transcriptional regulator alnR {ECO:0000303|PubMed:30339758};
DE AltName: Full=Asperlin biosynthesis cluster protein R {ECO:0000303|PubMed:30339758};
GN Name=alnR {ECO:0000303|PubMed:30339758}; ORFNames=AN9221;
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
RN [3]
RP GENE MODEL REVISION, IDENTIFICATION, DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=30339758; DOI=10.1021/acschembio.8b00679;
RA Grau M.F., Entwistle R., Chiang Y.M., Ahuja M., Oakley C.E., Akashi T.,
RA Wang C.C.C., Todd R.B., Oakley B.R.;
RT "Hybrid transcription factor engineering activates the silent secondary
RT metabolite gene cluster for (+)-asperlin in Aspergillus nidulans.";
RL ACS Chem. Biol. 13:3193-3205(2018).
CC -!- FUNCTION: Transcriptional regulator involved in the positive regulation
CC of the expression of the gene cluster that mediates the biosynthesis of
CC asperlin, a polyketide showing anti-inflammatory, antitumor and
CC antibiotic activities. {ECO:0000269|PubMed:30339758}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC -!- DISRUPTION PHENOTYPE: Strongly diminishes the production of asperlin.
CC {ECO:0000269|PubMed:30339758}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CBF82293.1; Type=Erroneous gene model prediction; Note=The predicted gene AN9221 has been split into 2 genes: alnG and alnR.; Evidence={ECO:0000305|PubMed:30339758};
CC Sequence=EAA61512.1; Type=Erroneous gene model prediction; Note=The predicted gene AN9221 has been split into 2 genes: alnG and alnR.; Evidence={ECO:0000305|PubMed:30339758};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AACD01000170; EAA61512.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BN001306; CBF82293.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_682490.1; XM_677398.1.
DR AlphaFoldDB; P0CU78; -.
DR SMR; P0CU78; -.
DR GeneID; 2867971; -.
DR KEGG; ani:AN9221.2; -.
DR Proteomes; UP000000560; Chromosome VI.
DR Proteomes; UP000005890; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 3: Inferred from homology;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..407
FT /note="Transcriptional regulator alnR"
FT /id="PRO_0000445946"
FT DNA_BIND 23..53
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 53..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 70..85
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 407 AA; 45078 MW; 7DC5F919677CDAA8 CRC64;
MSTVNQSSTR SELAGNWERL RKSCDTCQEA KVKCSQHKPS CHRCLRHRQP CVYSPQRRSG
RPPKRPSPSS RLGPESNNSG DDIHNENTIQ RTNLNANDSA MTDAGAVDPR VLTGDFAAST
GIDPVDDIFQ TSFESFLAAS LSPKGGLLPG SHSNPTTPNG FSMNSPSITD PFGAFPFLIT
DHNLPIAALS SHVPPIDQLP VLSTGASNTS SECGDCGAKC YSSLLQHLLF LRQTLPESTR
PSIDVIMQAE GHVRALLDRV LGCNACLGNR SSILLISAIT ERIVQMLDWI IEEKTLLDTE
NMRYNRRTFS SWGRPPRLPP HGLNGMRRNV CHVSLRVGNT ELDEDAKQYF LKNFILLRLK
KLAVKVQEVR RTATTRPGDC IYRAAELVLA DSIQRLDYLR GQCQLWE
