ID   ALNI_EMENI              Reviewed;         219 AA.
AC   C8VJQ9;
DT   05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT   03-NOV-2009, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Asperlin biosynthesis cluster protein I {ECO:0000303|PubMed:30339758};
GN   Name=alnI {ECO:0000303|PubMed:30339758}; ORFNames=ANIA_11200;
OS   Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS   M139) (Aspergillus nidulans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Nidulantes.
OX   NCBI_TaxID=227321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=16372000; DOI=10.1038/nature04341;
RA   Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA   Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA   Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA   Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA   Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA   Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA   Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA   Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA   Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT   "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT   fumigatus and A. oryzae.";
RL   Nature 438:1105-1115(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA   Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA   Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA   Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA   Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA   Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA   Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA   Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA   van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA   Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA   Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA   Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA   Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA   Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA   van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA   Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA   Oliver S.G., Turner G.;
RT   "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT   effort.";
RL   Fungal Genet. Biol. 46:S2-13(2009).
RN   [3]
RP   IDENTIFICATION, DISRUPTION PHENOTYPE, FUNCTION, INDUCTION, AND PATHWAY.
RX   PubMed=30339758; DOI=10.1021/acschembio.8b00679;
RA   Grau M.F., Entwistle R., Chiang Y.M., Ahuja M., Oakley C.E., Akashi T.,
RA   Wang C.C.C., Todd R.B., Oakley B.R.;
RT   "Hybrid transcription factor engineering activates the silent secondary
RT   metabolite gene cluster for (+)-asperlin in Aspergillus nidulans.";
RL   ACS Chem. Biol. 13:3193-3205(2018).
CC   -!- FUNCTION: Part of the gene cluster that mediates the biosynthesis of
CC       asperlin, a polyketide showing anti-inflammatory, antitumor and
CC       antibiotic activities (PubMed:30339758). The first step of the asperlin
CC       biosynthesis is the production of the intermediate 2,4,6-octatrienoic
CC       acid by the highly redusing polyketide synthase alnA with cleavage of
CC       the PKS product by the esterase alnB (PubMed:30339758). 2,4,6-
CC       octatrienoic acid is further converted to asperlin via several steps
CC       involving the remaining enzymes from the cluster (PubMed:30339758).
CC       {ECO:0000269|PubMed:30339758}.
CC   -!- PATHWAY: Polyketide biosynthesis. {ECO:0000269|PubMed:30339758}.
CC   -!- INDUCTION: Expression is controlled by the asperlin biosynthesis
CC       cluster-specific transcription factor alnR.
CC       {ECO:0000269|PubMed:30339758}.
CC   -!- DISRUPTION PHENOTYPE: Fully eliminates the production of asperlin.
CC       {ECO:0000269|PubMed:30339758}.
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DR   EMBL; BN001306; CBF82290.1; -; Genomic_DNA.
DR   AlphaFoldDB; C8VJQ9; -.
DR   EnsemblFungi; CBF82290; CBF82290; ANIA_11200.
DR   VEuPathDB; FungiDB:AN11200; -.
DR   eggNOG; ENOG502SZ1I; Eukaryota.
DR   HOGENOM; CLU_1245083_0_0_1; -.
DR   InParanoid; C8VJQ9; -.
DR   OMA; ICGCENS; -.
DR   OrthoDB; 1476434at2759; -.
DR   Proteomes; UP000000560; Chromosome VI.
PE   2: Evidence at transcript level;
KW   Reference proteome.
FT   CHAIN           1..219
FT                   /note="Asperlin biosynthesis cluster protein I"
FT                   /id="PRO_0000445951"
FT   REGION          97..124
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   219 AA;  22424 MW;  D9537FE44C22E7E4 CRC64;
     MFSSTRRAEG PCATELTQVS SLLPPRGPYE FSLLPTLTRP LEDLSKCIEG ARQTSATANG
     YSPTGLVPLA DSILEICQAA CTAYGLVDGA IAAGVGTGSS DNSPTATGIG AAGLTGDRPS
     SSGASTWRCV KTPMTLGSLT LQNEEESLLA RQIVYAVLTS LSALLREVYV REKDVVSETD
     VVGEGGVGAG AALYGREGAG AVSQCLSRVL ALLGKIVPE