ALMTC_ARATH
ID ALMTC_ARATH Reviewed; 560 AA.
AC O49696;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Aluminum-activated malate transporter 12;
DE Short=AtALMT12;
DE AltName: Full=Quick anion channel 1;
GN Name=ALMT12; Synonyms=QUAC1; OrderedLocusNames=At4g17970;
GN ORFNames=T6K21.150;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16740662; DOI=10.1073/pnas.0602868103;
RA Hoekenga O.A., Maron L.G., Pineros M.A., Cancado G.M., Shaff J.,
RA Kobayashi Y., Ryan P.R., Dong B., Delhaize E., Sasaki T., Matsumoto H.,
RA Yamamoto Y., Koyama H., Kochian L.V.;
RT "AtALMT1, which encodes a malate transporter, is identified as one of
RT several genes critical for aluminum tolerance in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:9738-9743(2006).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ALTERNATIVE SPLICING,
RP INDUCTION BY ALUMINUM, AND DISRUPTION PHENOTYPE.
RX PubMed=20154005; DOI=10.1093/pcp/pcq016;
RA Sasaki T., Mori I.C., Furuichi T., Munemasa S., Toyooka K., Matsuoka K.,
RA Murata Y., Yamamoto Y.;
RT "Closing plant stomata requires a homolog of an aluminum-activated malate
RT transporter.";
RL Plant Cell Physiol. 51:354-365(2010).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION BY
RP ALUMINUM.
RX PubMed=20626656; DOI=10.1111/j.1365-313x.2010.04302.x;
RA Meyer S., Mumm P., Imes D., Endler A., Weder B., Al-Rasheid K.A.,
RA Geiger D., Marten I., Martinoia E., Hedrich R.;
RT "AtALMT12 represents an R-type anion channel required for stomatal movement
RT in Arabidopsis guard cells.";
RL Plant J. 63:1054-1062(2010).
CC -!- FUNCTION: Malate-sensitive anion transporter permeable to chloride,
CC nitrate, sulfate and malate. Involved in dark-, CO(2)-, abscisic
CC acid- and water-deficient-induced stomatal closure. Belongs to the R-
CC type anion channels. {ECO:0000269|PubMed:20154005,
CC ECO:0000269|PubMed:20626656}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20154005,
CC ECO:0000269|PubMed:20626656}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:20154005, ECO:0000269|PubMed:20626656}.
CC Note=PubMed:20626656 indicates also a not confirmed endomembrane
CC localization.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Comment=The splice variants are not functional as anion
CC transporters.;
CC Name=1;
CC IsoId=O49696-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O49696-2; Sequence=VSP_040183, VSP_040184;
CC Name=3;
CC IsoId=O49696-3; Sequence=VSP_040185, VSP_040186;
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, leaves, flowers and
CC pollen. Mainly detected in the roots vascular stele and in the leaves
CC guard cells. {ECO:0000269|PubMed:20154005,
CC ECO:0000269|PubMed:20626656}.
CC -!- INDUCTION: Not induced by aluminum. {ECO:0000269|PubMed:20154005,
CC ECO:0000269|PubMed:20626656}.
CC -!- DISRUPTION PHENOTYPE: Impaired stomatal closure resulting in a wilty
CC phenotype. {ECO:0000269|PubMed:20154005}.
CC -!- MISCELLANEOUS: Malate functions as a gating modifier as well as a
CC permeating substrate.
CC -!- SIMILARITY: Belongs to the aromatic acid exporter (TC 2.A.85) family.
CC {ECO:0000305}.
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DR EMBL; AL021889; CAA17140.1; -; Genomic_DNA.
DR EMBL; AL161547; CAB78799.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE83973.1; -; Genomic_DNA.
DR PIR; T05083; T05083.
DR RefSeq; NP_193531.1; NM_117907.4. [O49696-1]
DR AlphaFoldDB; O49696; -.
DR SMR; O49696; -.
DR STRING; 3702.AT4G17970.1; -.
DR TCDB; 2.A.85.2.1; the aromatic acid exporter (arae) family.
DR PaxDb; O49696; -.
DR PRIDE; O49696; -.
DR ProteomicsDB; 244996; -. [O49696-1]
DR EnsemblPlants; AT4G17970.1; AT4G17970.1; AT4G17970. [O49696-1]
DR GeneID; 827522; -.
DR Gramene; AT4G17970.1; AT4G17970.1; AT4G17970. [O49696-1]
DR KEGG; ath:AT4G17970; -.
DR Araport; AT4G17970; -.
DR TAIR; locus:2141065; AT4G17970.
DR eggNOG; KOG4711; Eukaryota.
DR HOGENOM; CLU_020841_1_2_1; -.
DR InParanoid; O49696; -.
DR OMA; IACFKEF; -.
DR OrthoDB; 516124at2759; -.
DR PhylomeDB; O49696; -.
DR PRO; PR:O49696; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O49696; baseline and differential.
DR Genevisible; O49696; AT.
DR GO; GO:0012505; C:endomembrane system; IDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009705; C:plant-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008509; F:anion transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015743; P:malate transport; IEA:InterPro.
DR GO; GO:0010118; P:stomatal movement; IMP:TAIR.
DR GO; GO:0008272; P:sulfate transport; IEA:UniProtKB-KW.
DR InterPro; IPR020966; ALMT.
DR Pfam; PF11744; ALMT; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Chloride; Ion channel; Ion transport;
KW Membrane; Reference proteome; Sulfate transport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..560
FT /note="Aluminum-activated malate transporter 12"
FT /id="PRO_0000401471"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 130..150
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..176
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 386..421
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 270..277
FT /note="ALYANWEP -> VSLYYIFL (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_040183"
FT VAR_SEQ 278..560
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_040184"
FT VAR_SEQ 318..324
FT /note="TPRSVRA -> VRLLITL (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_040185"
FT VAR_SEQ 325..560
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_040186"
SQ SEQUENCE 560 AA; 62310 MW; 84587DFEB5100AE8 CRC64;
MSNKVHVGSL EMEEGLSKTK WMVLEPSEKI KKIPKRLWNV GKEDPRRVIH ALKVGLSLTL
VSLLYLMEPL FKGIGSNAIW AVMTVVVVLE FSAGATLCKG LNRGLGTLIA GSLAFFIEFV
ANDSGKVLRA IFIGTAVFII GAAATYIRFI PYIKKNYDYG VVIFLLTFNL ITVSSYRVDS
VINIAHDRFY TIAVGCGICL FMSLLVFPIW SGEDLHKTTV GKLQGLSRSI EACVDEYFEE
KEKEKTDSKD RIYEGYQAVL DSKSTDETLA LYANWEPRHT LRCHRFPCQQ YVKVGAVLRQ
FGYTVVALHG CLQTEIQTPR SVRALFKDPC VRLAGEVCKA LTELADSISN HRHCSPEILS
DHLHVALQDL NSAIKSQPKL FLGSNLHRHN NKHQNGSISN NKHHQRNSSN SGKDLNGDVS
LQNTETGTRK ITETGSRQGQ NGAVSLSSFR TDTSALMEYR RSFKNSNSEM SAAGERRMLR
PQLSKIAVMT SLEFSEALPF AAFASLLVEM VARLDNVIEE VEELGRIASF KEYDNKRDQT
ADDVRCENPA NVTISVGAAE
