ALGF_AZOVI
ID ALGF_AZOVI Reviewed; 217 AA.
AC P70799;
DT 25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 25-MAY-2022, entry version 52.
DE RecName: Full=Alginate biosynthesis protein AlgF;
DE Flags: Precursor;
GN Name=algF;
OS Azotobacter vinelandii.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Azotobacter.
OX NCBI_TaxID=354;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 9046;
RX PubMed=10352233; DOI=10.1016/s0378-1119(99)00119-5;
RA Vazquez-Ramos A., Moreno S., Guzman J., Alvarado A., Espin G.;
RT "Transcriptional organization of the Azotobacter vinelandii algGXLVIFA
RT genes: characterization of algF mutants.";
RL Gene 232:217-222(1999).
CC -!- FUNCTION: Together with AlgI and AlgJ, forms an inner membrane complex
CC which probably interacts with the alginate polymerization-transport
CC complex and adds acetyl groups at the O-2 and O-3 positions of
CC mannuronate residues. Acetylation of alginate increases cyst resistance
CC to desiccation.
CC -!- PATHWAY: Glycan biosynthesis; alginate biosynthesis.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the AlgF family. {ECO:0000305}.
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DR EMBL; X99019; CAA67490.1; -; Genomic_DNA.
DR RefSeq; WP_012699739.1; NZ_FPKM01000036.1.
DR AlphaFoldDB; P70799; -.
DR SMR; P70799; -.
DR OMA; SSDFSFM; -.
DR UniPathway; UPA00286; -.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0051979; P:alginic acid acetylation; IMP:CACAO.
DR GO; GO:0042121; P:alginic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR035422; AlgF.
DR Pfam; PF11182; AlgF; 1.
PE 3: Inferred from homology;
KW Alginate biosynthesis; Periplasm; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..217
FT /note="Alginate biosynthesis protein AlgF"
FT /id="PRO_0000001114"
SQ SEQUENCE 217 AA; 23195 MW; 368F64A1C4D81B5A CRC64;
MTRLHRKHRI ALGLGLGLLL GFGGTTVQAA GDAALYEAVA PKGSTFVRLY NASNQEVSAN
VGSINLEDVS PLSSSGFEYL PAGQYSARVG SQNLPVNLSA EHYYTLVTQS GAAPLLVEEP
AFKSKQKALL RVQNLSDKPL TLKTADGKTE VVKSVEPKGR GDREINPVKA NLALFDGERK
VADLKPISLA RGEVVCLYVT GTANQLNPVW VKPPVKE