ALGE_PSEAE
ID ALGE_PSEAE Reviewed; 490 AA.
AC P18895;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 08-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Alginate production protein AlgE;
DE Flags: Precursor;
GN Name=algE; Synonyms=alg76; OrderedLocusNames=PA3544;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 33-42.
RC STRAIN=8830;
RX PubMed=1743507; DOI=10.1016/0378-1119(91)90290-r;
RA Chu L., May T.B., Chakrabarty A.M., Misra T.K.;
RT "Nucleotide sequence and expression of the algE gene involved in alginate
RT biosynthesis by Pseudomonas aeruginosa.";
RL Gene 107:1-10(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [3]
RP CHARACTERIZATION.
RC STRAIN=CF3/M1;
RX PubMed=7521870; DOI=10.1128/jb.176.18.5639-5647.1994;
RA Rehm B.H., Boheim G., Tommassen J., Winkler U.K.;
RT "Overexpression of algE in Escherichia coli: subcellular localization,
RT purification, and ion channel properties.";
RL J. Bacteriol. 176:5639-5647(1994).
CC -!- FUNCTION: Has non-porin-like, channel-forming properties and probably
CC functions as an alginate permeability pore.
CC -!- PATHWAY: Glycan biosynthesis; alginate biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane; Peripheral membrane protein.
CC -!- SIMILARITY: Belongs to the AlgE family. {ECO:0000305}.
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DR EMBL; M37181; AAA25699.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG06932.1; -; Genomic_DNA.
DR PIR; E83202; E83202.
DR PIR; JS0586; JS0586.
DR RefSeq; NP_252234.1; NC_002516.2.
DR RefSeq; WP_003092105.1; NZ_QZGE01000001.1.
DR PDB; 3RBH; X-ray; 2.30 A; A/B/C/D=33-490.
DR PDB; 4AFK; X-ray; 1.90 A; A=33-490.
DR PDB; 4AZL; X-ray; 2.80 A; A/B=33-490.
DR PDB; 4B61; X-ray; 2.40 A; A/B=33-490.
DR PDB; 4XNK; X-ray; 2.80 A; A=1-490.
DR PDB; 4XNL; X-ray; 2.90 A; A=1-490.
DR PDB; 5D5D; X-ray; 2.40 A; A=1-490.
DR PDB; 5IYU; X-ray; 2.70 A; A=1-490.
DR PDB; 7ACG; X-ray; 1.85 A; A=33-490.
DR PDBsum; 3RBH; -.
DR PDBsum; 4AFK; -.
DR PDBsum; 4AZL; -.
DR PDBsum; 4B61; -.
DR PDBsum; 4XNK; -.
DR PDBsum; 4XNL; -.
DR PDBsum; 5D5D; -.
DR PDBsum; 5IYU; -.
DR PDBsum; 7ACG; -.
DR AlphaFoldDB; P18895; -.
DR SMR; P18895; -.
DR STRING; 287.DR97_4398; -.
DR TCDB; 1.B.13.1.1; the alginate export porin (aep) family.
DR PaxDb; P18895; -.
DR EnsemblBacteria; AAG06932; AAG06932; PA3544.
DR GeneID; 879838; -.
DR KEGG; pae:PA3544; -.
DR PATRIC; fig|208964.12.peg.3708; -.
DR PseudoCAP; PA3544; -.
DR HOGENOM; CLU_564755_0_0_6; -.
DR OMA; RFSEYRT; -.
DR PhylomeDB; P18895; -.
DR BioCyc; PAER208964:G1FZ6-3612-MON; -.
DR UniPathway; UPA00286; -.
DR EvolutionaryTrace; P18895; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042121; P:alginic acid biosynthetic process; IMP:PseudoCAP.
DR InterPro; IPR025388; Alginate_export_dom.
DR Pfam; PF13372; Alginate_exp; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alginate biosynthesis; Cell outer membrane;
KW Direct protein sequencing; Membrane; Reference proteome; Signal.
FT SIGNAL 1..32
FT /evidence="ECO:0000269|PubMed:1743507"
FT CHAIN 33..490
FT /note="Alginate production protein AlgE"
FT /id="PRO_0000020663"
FT REGION 102..121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 331..355
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 340..355
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 34
FT /note="N -> G (in Ref. 1; AAA25699)"
FT /evidence="ECO:0000305"
FT STRAND 41..54
FT /evidence="ECO:0007829|PDB:7ACG"
FT TURN 56..59
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 60..62
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 66..81
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 84..97
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 102..104
FT /evidence="ECO:0007829|PDB:4XNL"
FT HELIX 105..107
FT /evidence="ECO:0007829|PDB:4XNL"
FT HELIX 117..119
FT /evidence="ECO:0007829|PDB:4B61"
FT STRAND 123..135
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 138..140
FT /evidence="ECO:0007829|PDB:4AFK"
FT STRAND 144..154
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 158..160
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 162..173
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 175..185
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 191..193
FT /evidence="ECO:0007829|PDB:7ACG"
FT TURN 199..203
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 204..216
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 219..230
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 243..245
FT /evidence="ECO:0007829|PDB:5IYU"
FT STRAND 247..262
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 270..292
FT /evidence="ECO:0007829|PDB:7ACG"
FT TURN 293..295
FT /evidence="ECO:0007829|PDB:4XNK"
FT STRAND 297..319
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 321..333
FT /evidence="ECO:0007829|PDB:7ACG"
FT HELIX 338..340
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 358..361
FT /evidence="ECO:0007829|PDB:7ACG"
FT TURN 368..370
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 374..388
FT /evidence="ECO:0007829|PDB:7ACG"
FT TURN 389..391
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 392..405
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 406..408
FT /evidence="ECO:0007829|PDB:4XNK"
FT STRAND 412..414
FT /evidence="ECO:0007829|PDB:5D5D"
FT STRAND 425..438
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 439..441
FT /evidence="ECO:0007829|PDB:3RBH"
FT HELIX 448..451
FT /evidence="ECO:0007829|PDB:3RBH"
FT STRAND 455..467
FT /evidence="ECO:0007829|PDB:7ACG"
FT HELIX 469..471
FT /evidence="ECO:0007829|PDB:7ACG"
FT STRAND 473..475
FT /evidence="ECO:0007829|PDB:5D5D"
FT STRAND 479..489
FT /evidence="ECO:0007829|PDB:7ACG"
SQ SEQUENCE 490 AA; 54422 MW; 13B7A9627CE36C8C CRC64;
MNSSRSVNPR PSFAPRALSL AIALLLGAPA FAANSGEAPK NFGLDVKITG ESENDRDLGT
APGGTLNDIG IDLRPWAFGQ WGDWSAYFMG QAVAATDTIE TDTLQSDTDD GNNSRNDGRE
PDKSYLAARE FWVDYAGLTA YPGEHLRFGR QRLREDSGQW QDTNIEALNW SFETTLLNAH
AGVAQRFSEY RTDLDELAPE DKDRTHVFGD ISTQWAPHHR IGVRIHHADD SGHLRRPGEE
VDNLDKTYTG QLTWLGIEAT GDAYNYRSSM PLNYWASATW LTGDRDNLTT TTVDDRRIAT
GKQSGDVNAF GVDLGLRWNI DEQWKAGVGY ARGSGGGKDG EEQFQQTGLE SNRSNFTGTR
SRVHRFGEAF RGELSNLQAA TLFGSWQLRE DYDASLVYHK FWRVDDDSDI GTSGINAALQ
PGEKDIGQEL DLVVTKYFKQ GLLPASMSQY VDEPSALIRF RGGLFKPGDA YGPGTDSTMH
RAFVDFIWRF
