ALGD_PSESM
ID ALGD_PSESM Reviewed; 438 AA.
AC Q887P8;
DT 25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=GDP-mannose 6-dehydrogenase;
DE Short=GMD;
DE EC=1.1.1.132;
GN Name=algD; OrderedLocusNames=PSPTO_1243;
OS Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=223283;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC BAA-871 / DC3000;
RA Keith R.C., Keith L.M., Bender C.L.;
RT "Comparative analysis of the algD promoter, gene and protein from
RT Pseudomonas syringae, Pseudomonas aeruginosa, and Azotobacter vinelandii.";
RL Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA Collmer A.;
RT "The complete genome sequence of the Arabidopsis and tomato pathogen
RT Pseudomonas syringae pv. tomato DC3000.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
RN [3]
RP EXPRESSION IN HOST.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=12724374; DOI=10.1099/mic.0.26109-0;
RA Keith R.C., Keith L.M., Hernandez-Guzman G., Uppalapati S.R., Bender C.L.;
RT "Alginate gene expression by Pseudomonas syringae pv. tomato DC3000 in host
RT and non-host plants.";
RL Microbiology 149:1127-1138(2003).
CC -!- FUNCTION: Catalyzes the oxidation of guanosine diphospho-D-mannose
CC (GDP-D-mannose) to GDP-D-mannuronic acid, a precursor for alginate
CC polymerization. The alginate layer causes a mucoid phenotype and
CC provides a protective barrier against host immune defenses and
CC antibiotics (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GDP-alpha-D-mannose + H2O + 2 NAD(+) = GDP-alpha-D-mannuronate
CC + 3 H(+) + 2 NADH; Xref=Rhea:RHEA:21728, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57527, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:84886; EC=1.1.1.132;
CC -!- PATHWAY: Glycan biosynthesis; alginate biosynthesis.
CC -!- INDUCTION: By oxidative compounds such as H(2)O(2).
CC -!- SIMILARITY: Belongs to the UDP-glucose/GDP-mannose dehydrogenase
CC family. {ECO:0000305}.
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DR EMBL; AY095346; AAM23310.1; -; Genomic_DNA.
DR EMBL; AE016853; AAO54768.1; -; Genomic_DNA.
DR RefSeq; NP_791073.1; NC_004578.1.
DR RefSeq; WP_005764140.1; NC_004578.1.
DR AlphaFoldDB; Q887P8; -.
DR SMR; Q887P8; -.
DR STRING; 223283.PSPTO_1243; -.
DR EnsemblBacteria; AAO54768; AAO54768; PSPTO_1243.
DR GeneID; 1182879; -.
DR KEGG; pst:PSPTO_1243; -.
DR PATRIC; fig|223283.9.peg.1264; -.
DR eggNOG; COG1004; Bacteria.
DR HOGENOM; CLU_023810_1_1_6; -.
DR OMA; KYADNAF; -.
DR OrthoDB; 647136at2; -.
DR PhylomeDB; Q887P8; -.
DR UniPathway; UPA00286; -.
DR PHI-base; PHI:7031; -.
DR Proteomes; UP000002515; Chromosome.
DR GO; GO:0047919; F:GDP-mannose 6-dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0042121; P:alginic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR028358; GDPman_DH.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR017476; UDP-Glc/GDP-Man.
DR InterPro; IPR014027; UDP-Glc/GDP-Man_DH_C.
DR InterPro; IPR036220; UDP-Glc/GDP-Man_DH_C_sf.
DR InterPro; IPR014026; UDP-Glc/GDP-Man_DH_dimer.
DR InterPro; IPR001732; UDP-Glc/GDP-Man_DH_N.
DR Pfam; PF00984; UDPG_MGDP_dh; 1.
DR Pfam; PF03720; UDPG_MGDP_dh_C; 1.
DR Pfam; PF03721; UDPG_MGDP_dh_N; 1.
DR PIRSF; PIRSF500135; GDPman_DH; 1.
DR PIRSF; PIRSF000124; UDPglc_GDPman_dh; 1.
DR SMART; SM00984; UDPG_MGDP_dh_C; 1.
DR SUPFAM; SSF48179; SSF48179; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR SUPFAM; SSF52413; SSF52413; 1.
DR TIGRFAMs; TIGR03026; NDP-sugDHase; 1.
PE 2: Evidence at transcript level;
KW Alginate biosynthesis; NAD; Oxidoreductase; Reference proteome.
FT CHAIN 1..438
FT /note="GDP-mannose 6-dehydrogenase"
FT /id="PRO_0000074070"
FT ACT_SITE 268
FT /evidence="ECO:0000250"
FT SITE 10
FT /note="NAD binding"
FT /evidence="ECO:0000250"
FT SITE 11
FT /note="NAD binding"
FT /evidence="ECO:0000250"
FT SITE 30
FT /note="NAD binding"
FT /evidence="ECO:0000250"
FT SITE 35
FT /note="NAD binding"
FT /evidence="ECO:0000250"
FT SITE 86
FT /note="NAD binding"
FT /evidence="ECO:0000250"
FT SITE 161
FT /note="NAD binding"
FT /evidence="ECO:0000250"
FT SITE 271
FT /note="NAD binding"
FT /evidence="ECO:0000250"
FT SITE 331
FT /note="NAD binding"
FT /evidence="ECO:0000250"
SQ SEQUENCE 438 AA; 47608 MW; B027F5AEF9F06E16 CRC64;
MRISIFGLGY VGAVCAGCLS ARGHDVVGVD ISSTKIDLIN NGKSPIVEPG LEELLQKGLA
TGKLRGTTDF AEAIRATDLS MICVGTPSKK NGDLELDYIE SVCREIGYVL RDKNTRHTIV
VRSTVLPGTV ANVVIPILED CSGKKAGVDF GVAVNPEFLR ESTAIKDYDL PPMTVIGEFD
KASGDVLQSL YEELDAPIIR KDIAVAEMIK YTCNVWHATK VTFANEIGNI AKAVGVDGRE
VMDVVCQDKA LNLSQYYMRP GFAFGGSCLP KDVRALTYRA GSLDVDAPLL NSLMRSNTSQ
VQNAFDMVAS YDTRKVALLG LSFKAGTDDL RESPLVELAE MLIGKGFDLS IFDSNVEYAR
VHGANKDYIE SKIPHVSSLL NSDFDQVIND SDVIILGNRD ERFRSLANKT PEGKRVIDLV
GFMTNATTED GRAEGICW
