ID   ALGB_PSESM              Reviewed;         448 AA.
AC   Q88AQ2;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Alginate biosynthesis transcriptional regulatory protein AlgB;
GN   Name=algB; OrderedLocusNames=PSPTO_0334;
OS   Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=223283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-871 / DC3000;
RX   PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA   Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA   Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA   Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA   Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA   Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA   Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA   Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA   Collmer A.;
RT   "The complete genome sequence of the Arabidopsis and tomato pathogen
RT   Pseudomonas syringae pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC   -!- FUNCTION: Positive regulator of the alginate biosynthetic gene algD.
CC   -!- PATHWAY: Glycan biosynthesis; alginate biosynthesis [regulation].
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DR   EMBL; AE016853; AAO53879.1; -; Genomic_DNA.
DR   RefSeq; NP_790184.1; NC_004578.1.
DR   RefSeq; WP_003380235.1; NC_004578.1.
DR   AlphaFoldDB; Q88AQ2; -.
DR   SMR; Q88AQ2; -.
DR   STRING; 223283.PSPTO_0334; -.
DR   EnsemblBacteria; AAO53879; AAO53879; PSPTO_0334.
DR   GeneID; 61792575; -.
DR   KEGG; pst:PSPTO_0334; -.
DR   PATRIC; fig|223283.9.peg.350; -.
DR   eggNOG; COG2204; Bacteria.
DR   HOGENOM; CLU_000445_0_6_6; -.
DR   OMA; YRAVVMS; -.
DR   OrthoDB; 123059at2; -.
DR   PhylomeDB; Q88AQ2; -.
DR   UniPathway; UPA00286; -.
DR   Proteomes; UP000002515; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0042121; P:alginic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR   InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   3: Inferred from homology;
KW   Activator; Alginate biosynthesis; ATP-binding; DNA-binding;
KW   Nucleotide-binding; Phosphoprotein; Reference proteome; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..448
FT                   /note="Alginate biosynthesis transcriptional regulatory
FT                   protein AlgB"
FT                   /id="PRO_0000081006"
FT   DOMAIN          10..124
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DOMAIN          147..376
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   DNA_BIND        425..444
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   BINDING         175..182
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         238..247
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   MOD_RES         59
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   448 AA;  49513 MW;  C647430BD887AF7A CRC64;
     MEAATENQGR ILLVDDESAI LRTFRYCLED EGYSVATANS AAQADTLMQR QVFDLCFLDL
     RLGEDNGLDV LAQMRIQAPW MRVVIVTAHS AVDTAVDAIQ AGAADYLVKP CSPDQLRLAT
     AKQLEVRQLS ARLEALEGEV RKPKDGLDSH SPSMMAILET ARQVAVTDAN ILILGESGTG
     KGELARAIHG WSKRAKKSCV TINCPSLTAE LMESELFGHS RGAFTGASES TLGRVNQADG
     GTLFLDEIGD FPLTLQPKLL RFIQDKEYER VGDPVTRRAD VRILAATNLN LEDMVRSGRF
     REDLLYRLNV ITLHLPALRE RSEDILTLAD RFLARFVKEY ARPARGFSEE ARAALLNYRW
     PGNIRELRNV IERASIICPQ ERVEVSHLGM AEQPANNAPR VGAALSLDEL EKAHIGAVLA
     TSETLDQAAK TLGIDASTLY RKRKQYNL