ID   ALGB_PSEPK              Reviewed;         448 AA.
AC   Q88RJ6;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=Alginate biosynthesis transcriptional regulatory protein AlgB;
GN   Name=algB; OrderedLocusNames=PP_0133;
OS   Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS   / KT2440).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA   Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA   Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA   Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA   Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA   Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the metabolically
RT   versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
CC   -!- FUNCTION: Positive regulator of the alginate biosynthetic gene algD.
CC   -!- PATHWAY: Glycan biosynthesis; alginate biosynthesis [regulation].
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DR   EMBL; AE015451; AAN65767.1; -; Genomic_DNA.
DR   RefSeq; NP_742303.1; NC_002947.4.
DR   RefSeq; WP_010951528.1; NC_002947.4.
DR   AlphaFoldDB; Q88RJ6; -.
DR   SMR; Q88RJ6; -.
DR   STRING; 160488.PP_0133; -.
DR   PRIDE; Q88RJ6; -.
DR   EnsemblBacteria; AAN65767; AAN65767; PP_0133.
DR   KEGG; ppu:PP_0133; -.
DR   PATRIC; fig|160488.4.peg.135; -.
DR   eggNOG; COG2204; Bacteria.
DR   HOGENOM; CLU_000445_0_6_6; -.
DR   OMA; YRAVVMS; -.
DR   PhylomeDB; Q88RJ6; -.
DR   BioCyc; PPUT160488:G1G01-139-MON; -.
DR   UniPathway; UPA00286; -.
DR   Proteomes; UP000000556; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0042121; P:alginic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR002197; HTH_Fis.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR002078; Sigma_54_int.
DR   InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR   InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR   InterPro; IPR025944; Sigma_54_int_dom_CS.
DR   Pfam; PF02954; HTH_8; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00158; Sigma54_activat; 1.
DR   SMART; SM00382; AAA; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
DR   PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR   PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR   PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR   PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE   3: Inferred from homology;
KW   Activator; Alginate biosynthesis; ATP-binding; DNA-binding;
KW   Nucleotide-binding; Phosphoprotein; Reference proteome; Transcription;
KW   Transcription regulation; Two-component regulatory system.
FT   CHAIN           1..448
FT                   /note="Alginate biosynthesis transcriptional regulatory
FT                   protein AlgB"
FT                   /id="PRO_0000081005"
FT   DOMAIN          10..124
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DOMAIN          147..376
FT                   /note="Sigma-54 factor interaction"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   DNA_BIND        425..444
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   BINDING         175..182
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   BINDING         238..247
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT   MOD_RES         59
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   448 AA;  49605 MW;  14ECB103BD4BB2DF CRC64;
     MESAQDNQGR ILLVDDESAI LRTFRYCLED EGYSVATANS AAQAETLLQR QVFDLCFLDL
     RLGEDNGLDV LAQMRIQAPW MRVVIVTAHS AIDTAVDAIQ AGAADYLVKP CSPDQLRLAT
     AKQLEVRQLS ARLEALEGEI RKPKDGLDSH SPAMMAVLET ARQVAITDAN ILILGESGTG
     KGELARAIHG WSKRARKACV TINCPSLNAE LMESELFGHT RGAFTGASES TLGRVSQADG
     GTLFLDEIGD FPLTLQPKLL RFIQDKEYER VGDPVTRRAD VRILAATNLN LEEMVRESRF
     REDLLYRLNV ITLHLPPLRE RSEDILILAD RFLARFVKEY SRPARGFSDE ARTALLNYRW
     PGNIRELRNV VERASIICPQ ERVEISHLGM GEQPAGSAPR VGAALSLDEL ERAHIGAVLA
     ASDTLDQAAK TLGIDASTLY RKRKQYNL