ALGB_PSEAE
ID ALGB_PSEAE Reviewed; 449 AA.
AC P23747;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Alginate biosynthesis transcriptional regulatory protein AlgB;
GN Name=algB; OrderedLocusNames=PA5483;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=FRD1;
RX PubMed=1899859; DOI=10.1128/jb.173.4.1406-1413.1991;
RA Wozniak D.J., Ohman D.E.;
RT "Pseudomonas aeruginosa AlgB, a two-component response regulator of the
RT NtrC family, is required for algD transcription.";
RL J. Bacteriol. 173:1406-1413(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1738315; DOI=10.1111/j.1365-2958.1992.tb00837.x;
RA Goldberg J.B., Dahnke T.;
RT "Pseudomonas aeruginosa AlgB, which modulates the expression of alginate,
RT is a member of the NtrC subclass of prokaryotic regulators.";
RL Mol. Microbiol. 6:59-66(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [4]
RP CHARACTERIZATION, PHOSPHORYLATION AT ASP-59, AND MUTAGENESIS OF ASP-59.
RC STRAIN=FRD1;
RX PubMed=9473053; DOI=10.1128/jb.180.4.956-968.1998;
RA Ma S., Selvaraj U., Ohman D.E., Quarless R., Hassett D.J., Wozniak D.J.;
RT "Phosphorylation-independent activity of the response regulators AlgB and
RT AlgR in promoting alginate biosynthesis in mucoid Pseudomonas aeruginosa.";
RL J. Bacteriol. 180:956-968(1998).
CC -!- FUNCTION: Member of the two-component regulatory system AlgB/KinB
CC involved in regulation of alginate biosynthesis genes. Positive
CC regulator of the alginate biosynthetic gene AlgD.
CC -!- PATHWAY: Glycan biosynthesis; alginate biosynthesis [regulation].
CC -!- PTM: Phosphorylated by KinB. {ECO:0000269|PubMed:9473053}.
CC -!- MISCELLANEOUS: In vivo phosphorylation of AlgB is not required for its
CC role in alginate production. The mechanism by which it activates AlgD
CC appears not to be mediated by conventional phosphorylation-dependent
CC signal transduction.
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DR EMBL; M62902; AAA25700.1; -; Genomic_DNA.
DR EMBL; M82823; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AE004091; AAG08868.1; -; Genomic_DNA.
DR PIR; A38449; A38449.
DR RefSeq; NP_254170.1; NC_002516.2.
DR RefSeq; WP_003102840.1; NZ_QZGE01000012.1.
DR AlphaFoldDB; P23747; -.
DR SMR; P23747; -.
DR STRING; 287.DR97_2861; -.
DR PaxDb; P23747; -.
DR PRIDE; P23747; -.
DR EnsemblBacteria; AAG08868; AAG08868; PA5483.
DR GeneID; 877696; -.
DR KEGG; pae:PA5483; -.
DR PATRIC; fig|208964.12.peg.5748; -.
DR PseudoCAP; PA5483; -.
DR HOGENOM; CLU_000445_0_6_6; -.
DR InParanoid; P23747; -.
DR OMA; DMSPAIQ; -.
DR PhylomeDB; P23747; -.
DR BioCyc; PAER208964:G1FZ6-5610-MON; -.
DR UniPathway; UPA00286; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0042121; P:alginic acid biosynthetic process; IMP:PseudoCAP.
DR GO; GO:1900232; P:negative regulation of single-species biofilm formation on inanimate substrate; IMP:PseudoCAP.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:2000147; P:positive regulation of cell motility; IMP:PseudoCAP.
DR GO; GO:0045862; P:positive regulation of proteolysis; IMP:PseudoCAP.
DR GO; GO:1900378; P:positive regulation of secondary metabolite biosynthetic process; IMP:PseudoCAP.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:PseudoCAP.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR002197; HTH_Fis.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR002078; Sigma_54_int.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR025943; Sigma_54_int_dom_ATP-bd_2.
DR InterPro; IPR025944; Sigma_54_int_dom_CS.
DR Pfam; PF02954; HTH_8; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00158; Sigma54_activat; 1.
DR SMART; SM00382; AAA; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR PROSITE; PS00676; SIGMA54_INTERACT_2; 1.
DR PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE 1: Evidence at protein level;
KW Activator; Alginate biosynthesis; ATP-binding; DNA-binding;
KW Nucleotide-binding; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..449
FT /note="Alginate biosynthesis transcriptional regulatory
FT protein AlgB"
FT /id="PRO_0000081004"
FT DOMAIN 10..124
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 147..376
FT /note="Sigma-54 factor interaction"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT DNA_BIND 426..445
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT BINDING 175..182
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT BINDING 238..247
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT MOD_RES 59
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169,
FT ECO:0000269|PubMed:9473053"
FT MUTAGEN 59
FT /note="D->N: No phosphorylation; no effect on alginate
FT production."
FT /evidence="ECO:0000269|PubMed:9473053"
SQ SEQUENCE 449 AA; 49323 MW; E6452B88457CBC17 CRC64;
METTSEKQGR ILLVDDESAI LRTFRYCLED EGYSVATASS APQAEALLQR QVFDLCFLDL
RLGEDNGLDV LAQMRVQAPW MRVVIVTAHS AVDTAVDAMQ AGAVDYLVKP CSPDQLRLAA
AKQLEVRQLT ARLEALEDEV RRQGDGLESH SPAMAAVLET ARQVAATDAN ILILGESGSG
KGELARAIHT WSKRAKKPQV TINCPSLTAE LMESELFGHS RGAFTGATES TLGRVSQADG
GTLFLDEIGD FPLTLQPKLL RFIQDKEYER VGDPVTRRAD VRILAATNRD LGAMVAQGQF
REDLLYRLNV IVLNLPPLRE RAEDILGLAE RFLARFVKDY GRPARGFSEA AREAMRQYPW
PGNVRELRNV IERASIICNQ ELVDVDHLGF SAAQSASSAP RIGESLSLED LEKAHITAVM
ASSATLDQAA KTLGIDASTL YRKRKQYGL
