ID   ALG8_PSESM              Reviewed;         493 AA.
AC   Q887P9;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Glycosyltransferase alg8;
DE            EC=2.4.-.-;
GN   Name=alg8; OrderedLocusNames=PSPTO_1242;
OS   Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=223283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-871 / DC3000;
RX   PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA   Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA   Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA   Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA   Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA   Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA   Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA   Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA   Collmer A.;
RT   "The complete genome sequence of the Arabidopsis and tomato pathogen
RT   Pseudomonas syringae pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC   -!- FUNCTION: Possibly a processive enzyme that polymerizes GDP-mannuronic
CC       acid. {ECO:0000250}.
CC   -!- PATHWAY: Glycan biosynthesis; alginate biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
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DR   EMBL; AE016853; AAO54767.1; -; Genomic_DNA.
DR   RefSeq; NP_791072.1; NC_004578.1.
DR   RefSeq; WP_005764142.1; NC_004578.1.
DR   AlphaFoldDB; Q887P9; -.
DR   STRING; 223283.PSPTO_1242; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   EnsemblBacteria; AAO54767; AAO54767; PSPTO_1242.
DR   KEGG; pst:PSPTO_1242; -.
DR   PATRIC; fig|223283.9.peg.1263; -.
DR   eggNOG; COG1215; Bacteria.
DR   HOGENOM; CLU_024914_0_0_6; -.
DR   OMA; KLMFRWY; -.
DR   OrthoDB; 525208at2; -.
DR   PhylomeDB; Q887P9; -.
DR   UniPathway; UPA00286; -.
DR   Proteomes; UP000002515; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0042121; P:alginic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Alginate biosynthesis; Cell membrane; Glycosyltransferase; Membrane;
KW   Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..493
FT                   /note="Glycosyltransferase alg8"
FT                   /id="PRO_0000059256"
FT   TRANSMEM        13..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        47..69
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        380..402
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        422..444
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   493 AA;  55977 MW;  48EC48AF7923C82B CRC64;
     MQRLKHGLLQ AAGWLFYLSL LMGLAAALPT SIFDSQSKNF IFLIGAVGIW RYSMGITHFV
     RGMIFLYIVY PHLRRKVRKL GSAADPSHVF LMVTSFRIDA LTTAQVYSSV IREAIECGLP
     TTVVCSLVEM SDELLVKSMW AKANPPDRVK LDFVRIPGTG KRDGLAYGFR AISRHMPDDR
     AVVAVIDGDT VLNEGVVAKT VPWFQLFDNV GGLTTNEFCE VRGGYIMSEW HKLRFAQRHI
     NMCSMALSKR VLTMTGRMSV FRAKVVTDPE FIADVESDSL NHWRLGTFRF LTGDDKSSWF
     SLMRLGYDTF YVPDAAINTV EHPPEKSFLK ASRKLMYRWY GNNLRQNSRA LGLGVRRLGI
     FTSIVLFDQR VSMWTSILGL TVAIIASFKY GGAFLLMYLL WIGMTRLILT LLLSLSGHRI
     GPAYPMILYY NQIVGALMKI YVFFRLDRQS WTRQDTKLSR DMASFQGWFN TWSSRTMTFS
     AGTIFVAVLL TMV