ID   ALG8_PSEPK              Reviewed;         496 AA.
AC   Q88NC5;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Glycosyltransferase alg8;
DE            EC=2.4.-.-;
GN   Name=alg8; OrderedLocusNames=PP_1287;
OS   Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS   / KT2440).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA   Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA   Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA   Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA   Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA   Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the metabolically
RT   versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
CC   -!- FUNCTION: Possibly a processive enzyme that polymerizes GDP-mannuronic
CC       acid. {ECO:0000250}.
CC   -!- PATHWAY: Glycan biosynthesis; alginate biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
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DR   EMBL; AE015451; AAN66911.1; -; Genomic_DNA.
DR   RefSeq; NP_743447.1; NC_002947.4.
DR   AlphaFoldDB; Q88NC5; -.
DR   STRING; 160488.PP_1287; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   EnsemblBacteria; AAN66911; AAN66911; PP_1287.
DR   KEGG; ppu:PP_1287; -.
DR   PATRIC; fig|160488.4.peg.1364; -.
DR   eggNOG; COG1215; Bacteria.
DR   HOGENOM; CLU_024914_0_0_6; -.
DR   OMA; KLMFRWY; -.
DR   PhylomeDB; Q88NC5; -.
DR   BioCyc; PPUT160488:G1G01-1374-MON; -.
DR   UniPathway; UPA00286; -.
DR   Proteomes; UP000000556; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0042121; P:alginic acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   3: Inferred from homology;
KW   Alginate biosynthesis; Cell membrane; Glycosyltransferase; Membrane;
KW   Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..496
FT                   /note="Glycosyltransferase alg8"
FT                   /id="PRO_0000059255"
FT   TRANSMEM        9..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        51..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        383..405
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        425..447
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        471..493
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   496 AA;  56415 MW;  D7B5B9227067B5DB CRC64;
     MLNMQRLQTV LLQCAGWLLY MSLLMLIALA LPADIFDSQS KHFIFLVGAV GIWRYSMGAT
     HFIRGMIFLY GVYPYLRRKV QKMGNATDPS HVYLMVTSFR IEALTTAQVY SSVIREAINC
     GFPTTVVCSL VEMSDELLVK SLWAKYNPPA HVKLDIVRIA GTGKRDGLAY GFRAISRMLP
     DENAVVAVID GDTVLADGVV RKTVPWFKLF PNVGGLTTNE FCEVRGGYIM SEWHKLRFAQ
     RHINMCSMAL SKRVLTMTGR MSMFRASVVT NPEFIADVES DSLMHWRLGR FKFLTGDDKS
     SWFSLMRLGY DTFYVPDAAI NTVEHPPEKS FLKASRKLMY RWYGNNLRQN SRALGLGLRR
     LGLFTSIVLF DQRVSMWTSL LGLTVAVIAS LKFGMAFLLV YLLWIGITRL ILTIMLLCSG
     HNVGPAYPLI LYYNQIIGAL MKIYVFFRLD KQSWTRQPTA LKRDLASFQQ WFNTWSSRTM
     TFSAASIFVA VLFMVV