ALG13_SCHPO
ID ALG13_SCHPO Reviewed; 162 AA.
AC O14190;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 113.
DE RecName: Full=UDP-N-acetylglucosamine transferase subunit alg13;
DE EC=2.4.1.141;
DE AltName: Full=Asparagine-linked glycosylation protein 13;
GN Name=alg13; ORFNames=SPAC56E4.02c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: Involved in protein N-glycosylation. Essential for the second
CC step of the dolichol-linked oligosaccharide pathway (By similarity).
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N-acetyl-alpha-D-glucosaminyl-diphosphodolichol + UDP-N-
CC acetyl-alpha-D-glucosamine = H(+) + N,N'-diacetylchitobiosyl
CC diphosphodolichol + UDP; Xref=Rhea:RHEA:23380, Rhea:RHEA-COMP:9519,
CC Rhea:RHEA-COMP:9520, ChEBI:CHEBI:15378, ChEBI:CHEBI:57269,
CC ChEBI:CHEBI:57705, ChEBI:CHEBI:58223, ChEBI:CHEBI:58427;
CC EC=2.4.1.141;
CC -!- SUBUNIT: Heterodimer with alg14 to form a functional enzyme.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 28 family.
CC {ECO:0000305}.
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DR EMBL; CU329670; CAB16393.1; -; Genomic_DNA.
DR PIR; T38904; T38904.
DR RefSeq; NP_593269.1; NM_001018666.2.
DR AlphaFoldDB; O14190; -.
DR SMR; O14190; -.
DR BioGRID; 279774; 1.
DR STRING; 4896.SPAC56E4.02c.1; -.
DR CAZy; GT1; Glycosyltransferase Family 1.
DR MaxQB; O14190; -.
DR PaxDb; O14190; -.
DR EnsemblFungi; SPAC56E4.02c.1; SPAC56E4.02c.1:pep; SPAC56E4.02c.
DR GeneID; 2543352; -.
DR KEGG; spo:SPAC56E4.02c; -.
DR PomBase; SPAC56E4.02c; alg13.
DR VEuPathDB; FungiDB:SPAC56E4.02c; -.
DR eggNOG; KOG3349; Eukaryota.
DR HOGENOM; CLU_085408_2_2_1; -.
DR InParanoid; O14190; -.
DR OMA; NYLYYCK; -.
DR PhylomeDB; O14190; -.
DR Reactome; R-SPO-446193; Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
DR PRO; PR:O14190; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0004577; F:N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity; ISO:PomBase.
DR GO; GO:0006488; P:dolichol-linked oligosaccharide biosynthetic process; ISO:PomBase.
DR InterPro; IPR039042; Alg13-like.
DR InterPro; IPR007235; Glyco_trans_28_C.
DR PANTHER; PTHR12867; PTHR12867; 1.
DR Pfam; PF04101; Glyco_tran_28_C; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; Glycosyltransferase; Reference proteome;
KW Transferase.
FT CHAIN 1..162
FT /note="UDP-N-acetylglucosamine transferase subunit alg13"
FT /id="PRO_0000215603"
SQ SEQUENCE 162 AA; 17769 MW; E93A6A93FF52CB35 CRC64;
MNAFVTVGST QFDDLIRAVL KPEFQHCLVK HGINQLIVQY GKGKQAFGDP KSVAGLTILG
FDYAPEIESY IHDASIVISH AGAGSILQTL RSGKRLLVVP NESLMDNHQV ELATKLASMN
YLVTCSTSNL VEGLEELYPK ILTPFPKSDC STFQKVMQDV AR
