ID   ALG11_XENLA             Reviewed;         486 AA.
AC   Q08B22; Q3B8M7; Q4QQZ6;
DT   24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 2.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase;
DE            EC=2.4.1.131;
DE   AltName: Full=Asparagine-linked glycosylation protein 11 homolog;
DE   AltName: Full=Glycolipid 2-alpha-mannosyltransferase;
GN   Name=alg11;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Egg, Embryo, and Ovary;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-
CC         beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol + 2 GDP-alpha-
CC         D-mannose = alpha-D-Man-(1->2)-alpha-D-Man-(1->2)-alpha-D-Man-(1->3)-
CC         [alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-
CC         GlcNAc-diphosphodolichol + 2 GDP + 2 H(+); Xref=Rhea:RHEA:29523,
CC         Rhea:RHEA-COMP:12626, Rhea:RHEA-COMP:12627, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57527, ChEBI:CHEBI:58189, ChEBI:CHEBI:132511,
CC         ChEBI:CHEBI:132515; EC=2.4.1.131;
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q08B22-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q08B22-2; Sequence=VSP_026942;
CC   -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC       Glycosyltransferase 4 subfamily. {ECO:0000305}.
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DR   EMBL; BC097779; AAH97779.1; -; mRNA.
DR   EMBL; BC106203; AAI06204.1; -; mRNA.
DR   EMBL; BC124907; AAI24908.1; -; mRNA.
DR   RefSeq; NP_001116360.1; NM_001122888.1. [Q08B22-2]
DR   AlphaFoldDB; Q08B22; -.
DR   SMR; Q08B22; -.
DR   CAZy; GT4; Glycosyltransferase Family 4.
DR   MaxQB; Q08B22; -.
DR   DNASU; 733388; -.
DR   GeneID; 733388; -.
DR   KEGG; xla:733388; -.
DR   CTD; 733388; -.
DR   Xenbase; XB-GENE-5934162; alg11.L.
DR   Proteomes; UP000186698; Chromosome 2L.
DR   Bgee; 733388; Expressed in egg cell and 19 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004377; F:GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity; IEA:UniProtKB-EC.
DR   CDD; cd03806; GT4_ALG11-like; 1.
DR   InterPro; IPR038013; ALG11.
DR   InterPro; IPR031814; ALG11_N.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   PANTHER; PTHR45919; PTHR45919; 1.
DR   Pfam; PF15924; ALG11_N; 1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Glycosyltransferase; Membrane; Reference proteome;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..486
FT                   /note="GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-
FT                   mannosyltransferase"
FT                   /id="PRO_0000295620"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        230..250
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         398..486
FT                   /note="IVECMAAGTIILAHNSGGPKLDIVVPHEEQQTGFLADSVDSYAAAMDHILSL
FT                   TPEQRLSIRQNARLSVGRFSDQEFEANFLASSEPLFK -> ESLAILFCKHIIASINIL
FT                   LMDA (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_026942"
FT   CONFLICT        24
FT                   /note="T -> A (in Ref. 1; AAH97779)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        95
FT                   /note="V -> I (in Ref. 1; AAI06204)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        327
FT                   /note="A -> T (in Ref. 1; AAI06204)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        445
FT                   /note="H -> R (in Ref. 1; AAI06204)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   486 AA;  54709 MW;  36A3917D1479F946 CRC64;
     MAGPMCLCGM MRLLTALFIP VLITSVGLCL IFVLLFICTR LWVQRKKKVE IGKDGKKKKV
     VAFFHPYCNA GGGGERVLWC ALRSLQKRYK DAIYVIYTGD KDASEEQILN GAAARFNIKL
     SHPVRFIFLE KRGLVEASCY PRFTLLGQSL GSVVLGWEAL TKCVPDIYID SMGYAFTLPL
     FKYLGGCHVG CYVHYPTISM DMLSVVRSQH ARFNNAAFIS NNPVLSRLKL IYYYLFALFY
     GWVGSCSDVI MVNSTWTFSH ILDLWKCSDR TSIVYPPCDV QTFLEIDINQ HKENEEHSVV
     SIGQFRPEKD HPLQIRAFAA LLEKKTAEQR AKLKLILIGG CRNDEDELRV SELKKLSSEL
     GIPVEFKVNV PFEELKKHLS EATIGLHTMW NEHFGIGIVE CMAAGTIILA HNSGGPKLDI
     VVPHEEQQTG FLADSVDSYA AAMDHILSLT PEQRLSIRQN ARLSVGRFSD QEFEANFLAS
     SEPLFK