FUJ1_GIBF5
ID FUJ1_GIBF5 Reviewed; 2492 AA.
AC S0EET5;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2013, sequence version 1.
DT 03-AUG-2022, entry version 44.
DE RecName: Full=Polyketide synthase 19 {ECO:0000303|PubMed:23825955};
DE Short=PKS19 {ECO:0000303|PubMed:23825955};
DE EC=2.3.1.- {ECO:0000269|PubMed:26192387};
DE AltName: Full=Fujikurins biosynthesis cluster protein PKS19 {ECO:0000303|PubMed:26192387};
GN Name=PKS19 {ECO:0000303|PubMed:23825955}; ORFNames=FFUJ_12239;
OS Gibberella fujikuroi (strain CBS 195.34 / IMI 58289 / NRRL A-6831) (Bakanae
OS and foot rot disease fungus) (Fusarium fujikuroi).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC Fusarium fujikuroi species complex.
OX NCBI_TaxID=1279085;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION, FUNCTION,
RP AND INDUCTION.
RC STRAIN=CBS 195.34 / IMI 58289 / NRRL A-6831;
RX PubMed=23825955; DOI=10.1371/journal.ppat.1003475;
RA Wiemann P., Sieber C.M.K., von Bargen K.W., Studt L., Niehaus E.-M.,
RA Espino J.J., Huss K., Michielse C.B., Albermann S., Wagner D.,
RA Bergner S.V., Connolly L.R., Fischer A., Reuter G., Kleigrewe K., Bald T.,
RA Wingfield B.D., Ophir R., Freeman S., Hippler M., Smith K.M., Brown D.W.,
RA Proctor R.H., Muensterkoetter M., Freitag M., Humpf H.-U., Gueldener U.,
RA Tudzynski B.;
RT "Deciphering the cryptic genome: genome-wide analyses of the rice pathogen
RT Fusarium fujikuroi reveal complex regulation of secondary metabolism and
RT novel metabolites.";
RL PLoS Pathog. 9:E1003475-E1003475(2013).
RN [2]
RP FUNCTION.
RX PubMed=26192387; DOI=10.1021/np5008137;
RA von Bargen K.W., Niehaus E.M., Krug I., Bergander K., Wuerthwein E.U.,
RA Tudzynski B., Humpf H.U.;
RT "Isolation and structure elucidation of fujikurins A-D: products of the
RT PKS19 gene cluster in Fusarium fujikuroi.";
RL J. Nat. Prod. 78:1809-1815(2015).
CC -!- FUNCTION: Highly reducing polyketide synthase; part of the gene cluster
CC that mediates the biosynthesis of fujikurins A-D, secondary metabolites
CC playing a role during rice infection (PubMed:23825955,
CC PubMed:26192387). The polyketide synthase PKS19 acts with the trans-
CC enoyl reductase FFUJ_12240 and the polyketide transferase FFUJ_12241 to
CC produce fujikurins, however, the biosynthesis pathway has not been
CC identified yet (PubMed:23825955, PubMed:26192387).
CC {ECO:0000269|PubMed:23825955, ECO:0000269|PubMed:26192387}.
CC -!- INDUCTION: The fujikurins gene cluster is specifically expressed during
CC rice infection (PubMed:23825955). {ECO:0000269|PubMed:23825955}.
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DR EMBL; HF679030; CCT72377.1; -; Genomic_DNA.
DR AlphaFoldDB; S0EET5; -.
DR SMR; S0EET5; -.
DR STRING; 5127.CCT72377; -.
DR EnsemblFungi; CCT72377; CCT72377; FFUJ_12239.
DR VEuPathDB; FungiDB:FFUJ_12239; -.
DR HOGENOM; CLU_000022_31_0_1; -.
DR Proteomes; UP000016800; Chromosome 8.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0044550; P:secondary metabolite biosynthetic process; IEA:UniProt.
DR Gene3D; 1.10.1200.10; -; 1.
DR Gene3D; 3.10.129.110; -; 1.
DR Gene3D; 3.40.366.10; -; 1.
DR Gene3D; 3.40.47.10; -; 1.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR013217; Methyltransf_12.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR020807; PKS_dehydratase.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR016039; Thiolase-like.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR Pfam; PF16197; KAsynt_C_assoc; 1.
DR Pfam; PF00109; ketoacyl-synt; 1.
DR Pfam; PF02801; Ketoacyl-synt_C; 1.
DR Pfam; PF08659; KR; 1.
DR Pfam; PF08242; Methyltransf_12; 1.
DR Pfam; PF00550; PP-binding; 1.
DR Pfam; PF14765; PS-DH; 1.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00825; PKS_KS; 1.
DR SMART; SM00823; PKS_PP; 1.
DR SUPFAM; SSF47336; SSF47336; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR SUPFAM; SSF52151; SSF52151; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR SUPFAM; SSF53901; SSF53901; 1.
DR SUPFAM; SSF55048; SSF55048; 1.
DR PROSITE; PS00606; B_KETOACYL_SYNTHASE; 1.
DR PROSITE; PS50075; CARRIER; 1.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE 2: Evidence at transcript level;
KW Acyltransferase; Methyltransferase; Multifunctional enzyme; NADP;
KW Oxidoreductase; Phosphopantetheine; Phosphoprotein; Reference proteome;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..2492
FT /note="Polyketide synthase 19"
FT /id="PRO_0000442008"
FT DOMAIN 2404..2479
FT /note="Carrier"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT REGION 15..466
FT /note="Ketosynthase (KS) domain"
FT /evidence="ECO:0000255"
FT REGION 571..866
FT /note="Malonyl-CoA:ACP transacylase (MAT) domain"
FT /evidence="ECO:0000255"
FT REGION 967..1270
FT /note="Dehydrogenase (DH) domain"
FT /evidence="ECO:0000255"
FT REGION 1431..1604
FT /note="C-methyltransferase (CMeT) domain"
FT /evidence="ECO:0000255"
FT REGION 2118..2293
FT /note="Ketoreductase (KR) domain"
FT /evidence="ECO:0000255"
FT ACT_SITE 202
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10022"
FT MOD_RES 2438
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
SQ SEQUENCE 2492 AA; 272819 MW; FE92F5EC321772ED CRC64;
MGSQISEKYY AREPIAIVGT SCRFPGGASS PSKLWELLDN PRDVVQKIPP RRFSTEAFYN
ADSQHHGVSP SDLIKGLRLT LQWSTNVKHA YLLDDDPRGF DRDFFAINPK EAEAMDPQQR
ILLETVYESV ESAGYSIEQL RGSSTAVFVG CMSFDYQFAA IRGIDSTLPQ YHATGAAMSI
LANRVSYFYD WKGPSVAIDT ACSSSLVALH QAVSALRSGD AKLAVAAGSN LILGPEPFIS
ESKLNMLSPN GRSYMWDASA DGYTRGEGFS VLLLKTLTKA LADSDHIECV IRETGVNSDG
KTPGITMPSS NAQAQLIRDT YAKCGLDPNR ESDRPQFFEA HGTGTQAGDP IEARAIQEVF
FPDHTQPTDS KLLVGSVKTV IGHTEGTAGL AGVLKATLAI QHSQIPANLH FNELNPKIRP
YYDNLRIPTE TTPWPTLSPG APRRVSVNSF GFGGTNAHAI VESWDGPYVR ERSPAPNAIG
AGPFVLSANS SQALAANAGA LAEYLKEHPT TNLGQLGYTL FKRTNLPFRA AFSGSSVECL
VEKLQAGKES LKSNSRITTL PESLPPRVLG VFTGQGAQWA KMGEGLYQTS MVFRNSVEQM
QHSLDSLPES DRPNWTLAEQ LHAPKETSRV GQATVSQPLC TAIQVALVDV LHVVGVELSA
VVGHSSGEIG AAYAAGYLSA GDAIRIAYYR GFHSHLAQGP GGKRGSMMAV GLSYDQALSF
CNELSGGITV AASNSYTSCT LAGDAEVIEE AKSRLEENGT FARVLAVDTA YHSHHMKPCA
TPYLESLKQC DIQVQKPKKG CAWYSSVWGS DGSSRSFNTE GELLQGQYWI ENLTHPVLFS
QALARALNED QCFDCALEVG PHPALKGPSL EIIKTLTGVS LPYSGVLKRG EGAAEAFADA
LGLLWKSFPS SRPTITFNGI CRAFSAVKPS SMTILKNLPN YSWDHATLFW KESRASRNFR
TPKHAPHELL GRSVSHGEHS RREVHWRQVM RLRELPWLRG HKIQGEILFP ASGYLSMAYE
AAIRLVDEQQ SVRIVEMQDM DIVRAMRLED DTSGLEVLFT IHVTSQSQSC INANVACYSG
AVDAPQPLDA HQTELTAHFN GRIRLWLEQP SAYTLPLRSE PLLPMSELNM AQVYSSLSKE
GFDYSGDFQA KSMLRRLGHT VVTLSSPSPV SWTHTLTHPA PIDTAVQGLL TAFSFPGDGR
IGTTYLPTRI ESVRISTAPS ESDAPILKAD SVVTLTDVTT ITGDVDIFDA ASCHTQVQMR
GVQMTAIGKP PDRWLYAGKI WARDIAYGLE PGSGTQFSDD DRLLYEQLVR TAYFYLRQLR
SKIRPPELML MGKFRRHMMR WVTEHLFPQI ESGQHPDIRT EWKDDTLDTV QRWRNSRPAD
NNDMNLLHAM GQSLIPIVRG TVPPLKVLVQ DGMLDRLYFE GIGFRDGNVD LGAIVKQLGH
EHPRMRIVEV GAGTGGTTRT VLDALETRYA AYTYTDISTG FFENARSVFS QHASKLTFKT
LNIENDPVDQ GFTEGSFDML IASNCLHATH SLENTLRQCR KLLRPGGRLV LLEITRDFLP
IQMIMATLPG WFLGMDEGRL WAPTVTLDRW DEILKTTGFS GVDISSTPSY CSVIVSQAVD
ETVSVLRNPL ASPDGVPSLS EIVIIGGVGS GLAPQIQSLL TSTIPVETRI ASSLEDVEVN
RGSTVLCLGD LDSPSFCNMD QTRFEGLQKV FQAANAVLWV TSGATSGTNP EANITVGMGS
TLMAERGDLK LQFLDVDDPT TIDPSMLARM LLRLAILDQS KSDETLWTIE PELTLKNGAL
YIPRVQPLDV INRQSTARYR QVIEDIDLNS ASPVVDLVKY QDSWKLQSSP IDSTRVNGIE
VRVTASSLHT LSCEGGKPEY IFMGRELLSG DNIIGLSASN SSIANITDDQ VLHRWQDSQT
GAINVAQLSD LLAKALAENL LRNTTGPVWI HGAPSNLSEA IGEVTKEEGL DVFQTTSDLA
QVAAFNFIHP YATRQDIQDK QPSGLRNFVD LEHSELRNEA LHTSVAASLP VSAISHRCMM
DLTHGINRSY LAKLAARCLT EDRKSSEDHS QVILIDRIAS ESSKDLGPTT VIDWHAANTV
TALVRPLEHR DLFFPSKSYL LFGMTGDLGI SVCKWMVDNG ARNVVLASRN PNVPSSVLNF
MSRKSATVRA LSVDITDMES LCTAREDIES TMPPIGGVMN AAMVLRDRLF HDLTWEDFAA
VLGPKVQGTQ NINNAFGQKE SALDFFVCFS SATSIIGTVG QSAYAAANHF MVSLVQQRKR
RGLAGSIVHI SVLTGLGYIF RRGSEHTAVI DKALLPRLER QAETDLHEML AEAIVCGRPG
SSQPPELITG IKAVFQGEWR DDPRLLGYLG QQQLGNDSAK DQAAGMVSVE TQLDAADDPA
ECLPILEKRF AQALGNMLEI DPEKVDGSMP VASLGIDSLV AIRIREWFMK ELGVEVPVLK
IMSVGHSLSR VCDDVLVDWR RVKKEGELED KK
