FUI1_YEAST
ID FUI1_YEAST Reviewed; 639 AA.
AC P38196; D6VPV6;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Uridine permease;
GN Name=FUI1; OrderedLocusNames=YBL042C; ORFNames=YBL0406;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7871888; DOI=10.1002/yea.320101113;
RA de Wergifosse P., Jacques B., Jonniaux J.-L., Purnelle B., Skala J.,
RA Goffeau A.;
RT "The sequence of a 22.4 kb DNA fragment from the left arm of yeast
RT chromosome II reveals homologues to bacterial proline synthetase and murine
RT alpha-adaptin, as well as a new permease and a DNA-binding protein.";
RL Yeast 10:1489-1496(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP CHARACTERIZATION.
RX PubMed=9485596; DOI=10.1111/j.1574-6968.1998.tb12843.x;
RA Wagner R., de Montigny J., de Wergifosse P., Souciet J.-L., Potier S.;
RT "The ORF YBL042 of Saccharomyces cerevisiae encodes a uridine permease.";
RL FEMS Microbiol. Lett. 159:69-75(1998).
RN [5]
RP CHARACTERIZATION.
RX PubMed=10827169; DOI=10.1074/jbc.m000239200;
RA Vickers M.F., Yao S.Y., Baldwin S.A., Young J.D., Cass C.E.;
RT "Nucleoside transporter proteins of Saccharomyces cerevisiae. Demonstration
RT of a transporter (FUI1) with high uridine selectivity in plasma membranes
RT and a transporter (FUN26) with broad nucleoside selectivity in
RT intracellular membranes.";
RL J. Biol. Chem. 275:25931-25938(2000).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-54 AND SER-56, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [9]
RP UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-635, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22106047; DOI=10.1002/pmic.201100166;
RA Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.;
RT "Sites of ubiquitin attachment in Saccharomyces cerevisiae.";
RL Proteomics 12:236-240(2012).
CC -!- FUNCTION: High-affinity transport of uridine.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the purine-cytosine permease (2.A.39) family.
CC {ECO:0000305}.
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DR EMBL; X78214; CAA55059.1; -; Genomic_DNA.
DR EMBL; Z35803; CAA84862.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07076.1; -; Genomic_DNA.
DR PIR; S45776; S45776.
DR RefSeq; NP_009511.1; NM_001178282.1.
DR AlphaFoldDB; P38196; -.
DR SMR; P38196; -.
DR BioGRID; 32655; 53.
DR DIP; DIP-1321N; -.
DR IntAct; P38196; 6.
DR MINT; P38196; -.
DR STRING; 4932.YBL042C; -.
DR TCDB; 2.A.39.3.3; the nucleobase:cation symporter-1 (ncs1) family.
DR iPTMnet; P38196; -.
DR MaxQB; P38196; -.
DR PaxDb; P38196; -.
DR PRIDE; P38196; -.
DR EnsemblFungi; YBL042C_mRNA; YBL042C; YBL042C.
DR GeneID; 852238; -.
DR KEGG; sce:YBL042C; -.
DR SGD; S000000138; FUI1.
DR VEuPathDB; FungiDB:YBL042C; -.
DR eggNOG; KOG2466; Eukaryota.
DR GeneTree; ENSGT00940000176299; -.
DR HOGENOM; CLU_021555_2_2_1; -.
DR InParanoid; P38196; -.
DR OMA; MLVLLKF; -.
DR BioCyc; YEAST:G3O-28943-MON; -.
DR PRO; PR:P38196; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38196; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0015205; F:nucleobase transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015213; F:uridine transmembrane transporter activity; IDA:SGD.
DR GO; GO:0015851; P:nucleobase transport; IBA:GO_Central.
DR GO; GO:0055085; P:transmembrane transport; IMP:SGD.
DR GO; GO:0015862; P:uridine transport; IDA:SGD.
DR InterPro; IPR012681; NCS1.
DR InterPro; IPR001248; Pur-cyt_permease.
DR InterPro; IPR045225; Uracil/uridine/allantoin_perm.
DR PANTHER; PTHR30618; PTHR30618; 1.
DR Pfam; PF02133; Transp_cyt_pur; 1.
DR TIGRFAMs; TIGR00800; ncs1; 1.
PE 1: Evidence at protein level;
KW Isopeptide bond; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Ubl conjugation.
FT CHAIN 1..639
FT /note="Uridine permease"
FT /id="PRO_0000197923"
FT TOPO_DOM 1..162
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 181..200
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..225
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 226..259
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 277..283
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 306..367
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 368..392
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 393..416
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..435
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 436..460
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 461..477
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 478..483
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 484..507
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 508..537
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 538..562
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 563..572
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 573..590
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 591..639
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..37
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 54
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT MOD_RES 56
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17330950"
FT CROSSLNK 635
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0007744|PubMed:22106047"
SQ SEQUENCE 639 AA; 72165 MW; 757325DEF4100399 CRC64;
MPVSDSGFDN SSKTMKDDTI PTEDYEEITK ESEMGDATKI TSKIDANVIE KKDTDSENNI
TIAQDDEKVS WLQRVVEFFE VKNDSTDLAD HKPENPIRTF KDLQESLRST YLYNTDLRPV
EAKRRTWTWK QYIFFWISGS FNVNTWQISA TGLQLGLNWW QTWICIWVGY TFVAFFLILG
SKVGNNYHIS FPISSRVSFG IYFSIWIVIN RVVMACVWNS TLAYIGSQCV QLMLKAIFGT
NLNTRIKDTI KNPNLTNFEF MCFMVFWVAC LPFLWFPPDK LRHIFALKSA ITPFAAFGFL
IWTLCKAKGH LALGSLNDNG GAISKTVLAW SVIRAIMSAL DNFSTLILNA PDFTRFGKTY
KSSVYSQLIA LPVCYAIISL IGILSVSAAY TLYGVNYWSP LDILNRYLDN YTSGNRAGVF
LISFIFAFDQ LGANLSGNSI PAGTDLTALL PKFINIRRGS YICALISLAI CPWDLLSSSS
KFTTALAAYA VFLSAIAGVI SADYFIVRKG YVNIFHCYTD KPGSYYMYNK YGTNWRAVVA
YIFGIAPNFA GFLGSVGVSV PIGAMKVYYL NYFVGYLLAA LSYCILVYFY PIKGIPGDAK
ITDRKWLEEW VEVEEFGTER EAFEEYGGVS TGYEKIRYI
