FUCM_XANCP
ID FUCM_XANCP Reviewed; 125 AA.
AC Q8P3K1;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 83.
DE RecName: Full=L-fucose mutarotase {ECO:0000303|PubMed:17144652};
DE EC=5.1.3.29;
DE AltName: Full=Fucose 1-epimerase {ECO:0000250|UniProtKB:P0AEN8};
DE AltName: Full=Type-2 mutarotase {ECO:0000250|UniProtKB:P0AEN8};
GN OrderedLocusNames=XCC4070;
OS Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB
OS 528 / LMG 568 / P 25).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Xanthomonas.
OX NCBI_TaxID=190485;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25;
RX PubMed=12024217; DOI=10.1038/417459a;
RA da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R.,
RA Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr.,
RA Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G.,
RA Cannavan F., Cardozo J., Chambergo F., Ciapina L.P., Cicarelli R.M.B.,
RA Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B.,
RA Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F.,
RA Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T.,
RA Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A.,
RA Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J.,
RA Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M.,
RA Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A.,
RA Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A.,
RA Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M.,
RA Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P.;
RT "Comparison of the genomes of two Xanthomonas pathogens with differing host
RT specificities.";
RL Nature 417:459-463(2002).
RN [2] {ECO:0000305}
RP FUNCTION, CATALYTIC ACTIVITY, ACTIVITY REGULATION, AND BIOPHYSICOCHEMICAL
RP PROPERTIES.
RC STRAIN=ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25
RC {ECO:0000269|PubMed:17144652};
RX PubMed=17144652; DOI=10.1021/bi061687o;
RA Yew W.S., Fedorov A.A., Fedorov E.V., Rakus J.F., Pierce R.W., Almo S.C.,
RA Gerlt J.A.;
RT "Evolution of enzymatic activities in the enolase superfamily: L-fuconate
RT dehydratase from Xanthomonas campestris.";
RL Biochemistry 45:14582-14597(2006).
CC -!- FUNCTION: Plays a role in the catabolism of L-fucose. Involved in the
CC anomeric conversion of L-fucose. {ECO:0000269|PubMed:17144652}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=alpha-L-fucose = beta-L-fucose; Xref=Rhea:RHEA:25580,
CC ChEBI:CHEBI:42548, ChEBI:CHEBI:42589; EC=5.1.3.29;
CC Evidence={ECO:0000269|PubMed:17144652};
CC -!- ACTIVITY REGULATION: Active toward L-galactopyranoside and D-
CC arabinopyranoside but no D-fucopyranoside activity detected.
CC {ECO:0000269|PubMed:17144652}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=8.5 mM for L-fucose {ECO:0000269|PubMed:17144652};
CC -!- SIMILARITY: Belongs to the RbsD / FucU family. FucU mutarotase
CC subfamily. {ECO:0000255}.
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DR EMBL; AE008922; AAM43291.1; -; Genomic_DNA.
DR RefSeq; NP_639409.1; NC_003902.1.
DR RefSeq; WP_011039139.1; NC_003902.1.
DR AlphaFoldDB; Q8P3K1; -.
DR SMR; Q8P3K1; -.
DR STRING; 340.xcc-b100_4272; -.
DR EnsemblBacteria; AAM43291; AAM43291; XCC4070.
DR KEGG; xcc:XCC4070; -.
DR PATRIC; fig|190485.4.peg.4362; -.
DR eggNOG; COG3254; Bacteria.
DR HOGENOM; CLU_100689_4_0_6; -.
DR OMA; LMWKYQQ; -.
DR Proteomes; UP000001010; Chromosome.
DR GO; GO:0036373; F:L-fucose mutarotase activity; IEA:UniProtKB-EC.
DR InterPro; IPR011008; Dimeric_a/b-barrel.
DR InterPro; IPR008000; Rham/fucose_mutarotase.
DR Pfam; PF05336; rhaM; 1.
DR SUPFAM; SSF54909; SSF54909; 1.
PE 1: Evidence at protein level;
KW Isomerase; Reference proteome.
FT CHAIN 1..125
FT /note="L-fucose mutarotase"
FT /id="PRO_0000419048"
FT ACT_SITE 13
FT /note="Proton donor"
FT /evidence="ECO:0000250"
SQ SEQUENCE 125 AA; 13959 MW; 233B4D70A36FBBB9 CRC64;
MSMQRLCYVL DLHDDAALIA QYERWHRPSE VWPEVVASLQ QAGIAELEIF RSGDRLVMLM
TVGEDYDPAA KAARDAGDPR IQAWEALMWR FQKALPGSAP GEKWREAGRI FALSEAVSVQ
QGSAA
