FTHS_SHESH
ID FTHS_SHESH Reviewed; 570 AA.
AC A8FQV1;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 1.
DT 03-AUG-2022, entry version 80.
DE RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543}; OrderedLocusNames=Ssed_0612;
OS Shewanella sediminis (strain HAW-EB3).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC Shewanellaceae; Shewanella.
OX NCBI_TaxID=425104;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HAW-EB3;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C.,
RA Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.,
RA Zhao J.-S., Richardson P.;
RT "Complete sequence of Shewanella sediminis HAW-EB3.";
RL Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-
CC formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:57454, ChEBI:CHEBI:456216; EC=6.3.4.3;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
CC -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR EMBL; CP000821; ABV35224.1; -; Genomic_DNA.
DR RefSeq; WP_012140961.1; NC_009831.1.
DR AlphaFoldDB; A8FQV1; -.
DR SMR; A8FQV1; -.
DR STRING; 425104.Ssed_0612; -.
DR EnsemblBacteria; ABV35224; ABV35224; Ssed_0612.
DR KEGG; sse:Ssed_0612; -.
DR eggNOG; COG2759; Bacteria.
DR HOGENOM; CLU_003601_3_3_6; -.
DR OMA; CGEIMTM; -.
DR OrthoDB; 177859at2; -.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000002015; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00477; FTHFS; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_01543; FTHFS; 1.
DR InterPro; IPR000559; Formate_THF_ligase.
DR InterPro; IPR020628; Formate_THF_ligase_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF01268; FTHFS; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00721; FTHFS_1; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT CHAIN 1..570
FT /note="Formate--tetrahydrofolate ligase"
FT /id="PRO_1000087658"
FT BINDING 65..72
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ SEQUENCE 570 AA; 60653 MW; 9D17A7AD4F060564 CRC64;
MLTDIEISRQ TKHRPITQIA EKFGITSSEL VPFGDAKAKV KLSILKRVDD NLEGKLVIVT
AVTPTPFGEG KTVTSIGLTQ GLNALGKKAC ACIRQPSMGP VFGIKGGAAG GGYSQVVPME
ELNLHLTGDI HAVSSAHNLA AAAIDARLFH ESRMTADEYR EQSGQAPLNI DPEKILWRRV
VDHNERSLRN ITVGLGANNG PIHDSGFDIT AASELMAILA LSLDLKDMRR RIGKLVLALD
SLGQPITAET LGVAGAMTVI MSDAIEPTLM QTLTGDPCLI HAGPFANIAH GNSSIIADRI
ALKLADIVVT EGGFGSDMGF EKFCNIKTRV SGKTPDAAVV VVTLKALKAN SGIDSQNDIN
RPDMARLQVG FANLKWHIDN VSQYGAPVVV AINRFPTDTF EELTWLKAKV LETAAFGCEI
CEGFSQGAKG AEALARVVVS ATEQKSDFKF LYQLDESIES KLLTIAEAGY GAAGIKLSPD
AKIQLQEINS MGLDKLAVCV AKTPLSISHE PKVKGVPTDF ELPITQLKIN AGAGFITALV
GKVMTMPGLG IKPGYLNVDI NEDDEIVGLA
