ID   ALDHX_YEASX             Reviewed;         533 AA.
AC   P22281;
DT   01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Aldehyde dehydrogenase 1, mitochondrial;
DE            EC=1.2.1.3;
DE   Flags: Precursor;
GN   Name=ALD1; Synonyms=ALDH, ALDH1;
OS   Saccharomyces cerevisiae (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=4932;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2022618; DOI=10.1128/jb.173.10.3199-3208.1991;
RA   Saigal D., Cunningham S.J., Farres J., Weiner H.;
RT   "Molecular cloning of the mitochondrial aldehyde dehydrogenase gene of
RT   Saccharomyces cerevisiae by genetic complementation.";
RL   J. Bacteriol. 173:3199-3208(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an aldehyde + H2O + NAD(+) = a carboxylate + 2 H(+) + NADH;
CC         Xref=Rhea:RHEA:16185, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:29067, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.2.1.3;
CC   -!- PATHWAY: Alcohol metabolism; ethanol degradation; acetate from ethanol:
CC       step 2/2.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC       {ECO:0000305}.
CC   -!- CAUTION: Is not present in yeast genome. {ECO:0000305}.
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DR   EMBL; M57887; AAA34419.1; -; Genomic_DNA.
DR   PIR; A39410; A39410.
DR   AlphaFoldDB; P22281; -.
DR   SMR; P22281; -.
DR   BindingDB; P22281; -.
DR   ChEMBL; CHEMBL5923; -.
DR   PRIDE; P22281; -.
DR   SGD; S000029651; ALD1.
DR   VEuPathDB; FungiDB:YER073W; -.
DR   VEuPathDB; FungiDB:YKR096W; -.
DR   UniPathway; UPA00780; UER00768.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0004029; F:aldehyde dehydrogenase (NAD+) activity; IEA:UniProtKB-EC.
DR   GO; GO:0043878; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006068; P:ethanol catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.309.10; -; 1.
DR   Gene3D; 3.40.605.10; -; 1.
DR   InterPro; IPR016161; Ald_DH/histidinol_DH.
DR   InterPro; IPR016163; Ald_DH_C.
DR   InterPro; IPR016160; Ald_DH_CS_CYS.
DR   InterPro; IPR029510; Ald_DH_CS_GLU.
DR   InterPro; IPR016162; Ald_DH_N.
DR   InterPro; IPR015590; Aldehyde_DH_dom.
DR   Pfam; PF00171; Aldedh; 1.
DR   SUPFAM; SSF53720; SSF53720; 1.
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE   3: Inferred from homology;
KW   Mitochondrion; NAD; Oxidoreductase; Transit peptide.
FT   TRANSIT         1..21
FT                   /note="Mitochondrion"
FT   CHAIN           22..533
FT                   /note="Aldehyde dehydrogenase 1, mitochondrial"
FT                   /id="PRO_0000007163"
FT   ACT_SITE        289
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        323
FT                   /evidence="ECO:0000250"
FT   BINDING         266..271
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   533 AA;  59507 MW;  B5718E2328A21A0B CRC64;
     MLATRNLVPI IRASIKWRIK LSALHYCMSD AETSEALLED NSAYINNEKH NLFLEKIFSD
     YQPFKHDNRT QVSCSQHMRD YRPLLTLSSA TRSVLFSLLA SDMSIILSIS PNTGILLCIG
     HLLASDIEDV VIVLSRGSPL VDLASTRIFK LAQNGTLRFA IKRTTFQELR FLRKSKDENV
     MEAATRGIIT IRQLYYENKV LPLRFTGNVA THIEENLEFE EQITWRTHVD SSIFPNTRCA
     YPSGYGPSAK IPCLSHKPND ILAYTGSTLV GRVVSKLAPE QVMKKVTLES GGKSTMAVFI
     QHDVTWAVEN TQFGVFDRQG QCCIAQSGYT VHRSTLSQIV ENNLEKDPSY VLHVDTESDI
     RGPFILKIHF ESIPRRINSA KAENSKVLCG GPRENSVYLY PTLSATLTDE CRIMKEEVFA
     PIITILCVKT VDEAIQRGNN SKFGLAAYVT KENVHGIILS TALKTVKLFI ICVHLASYQI
     PFGGNKNSGM GAELGKRALE NYTEGNHVLP VSLVKETLAP NTETASPARW PIH